| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2013-08-22 |
| タイトル |
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タイトル |
Novel photosensory two-component system (PixA-NixB-NixC) involved in the regulation of positive and negative phototaxis of cyanobacterium synechocystis sp. PCC 6803 |
| タイトル |
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タイトル |
Novel photosensory two-component system (PixA-NixB-NixC) involved in the regulation of positive and negative phototaxis of cyanobacterium synechocystis sp. PCC 6803 |
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言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
metadata only access |
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アクセス権URI |
http://purl.org/coar/access_right/c_14cb |
| 著者 |
NARIKAWA, Rei
SUZUKI, Fumiko
YOSHIHARA, Shizue
HIGASHI, Sho-ichi
WATANABE, Masakatsu
IKEUCHI, Masahiko
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| 著者別名 |
渡辺, 正勝
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Two wild-type substrains of a motile cyanobacterium Synechocystis sp. PCC 6803 show positive phototaxis toward a light source (PCC-P) and negative phototaxis away from light (PCC-N). In this study, we found that a novel two-component system of photoresponse is involved in the phototactic regulation. Inactivation of slr1212 (pixA), which encodes a photoreceptor histidine kinase, reverted the positive phototaxis of PCC-P to negative phototaxis, and inactivation of the downstream slr1213 (nixB) and slr1214 (nixC), which encode AraC-like transcription factor-type and PatA-type response regulators, respectively, reverted the negative phototaxis of PCC-N to positive phototaxis. Opposite effects of pixA and nixBC disruption implies an unexpected signal transduction pathway in the switching of positive and negative phototaxis. The blue/green-type cyanobacteriochrome GAF domain of PixA was expressed in Synechocystis and phycocyanobilin-producing Escherichia coli. The holoprotein covalently bound a chromophore phycoviolobilin and showed reversible photoconversion between the violet-(Pv, λ peak = 396 nm) and green-absorbing (Pg, λ peak = 533 nm) forms, although the protein from E. coli partially bound a precursor phycocyanobilin. These results were discussed with regard to an idea that PixA serves as a violet light receptor for switching of positive and negative phototaxis by transcriptional and functional regulation. |
| 書誌情報 |
Plant and Cell Physiology
en : Plant and Cell Physiology
巻 52,
号 12,
p. 2214-2224,
発行日 2011-12
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| 出版者 |
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出版者 |
Oxford University Press |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
00320781 |
| PubMed番号 |
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識別子タイプ |
PMID |
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関連識別子 |
22065076 |
| DOI |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1093/pcp/pcr155 |
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関連名称 |
10.1093/pcp/pcr155 |
| 権利 |
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権利情報 |
© 2011 The Author |
