{"_buckets": {"deposit": "815e2d0a-1d8d-4c0f-b3a0-96a95dfa2f27"}, "_deposit": {"created_by": 21, "id": "3911", "owners": [21], "pid": {"revision_id": 0, "type": "depid", "value": "3911"}, "status": "published"}, "_oai": {"id": "oai:ir.soken.ac.jp:00003911", "sets": ["283"]}, "author_link": ["345", "1970", "1971", "1868", "836", "1875", "346"], "item_10001_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2011-12", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "12", "bibliographicPageEnd": "2224", "bibliographicPageStart": "2214", "bibliographicVolumeNumber": "52", "bibliographic_titles": [{"bibliographic_title": "Plant and Cell Physiology "}, {"bibliographic_title": "Plant and Cell Physiology ", "bibliographic_titleLang": "en"}]}]}, "item_10001_creator_3": {"attribute_name": "著者別名", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "渡辺, 正勝"}], "nameIdentifiers": [{"nameIdentifier": "345", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "1000040124226", "nameIdentifierScheme": "NRID", "nameIdentifierURI": " "}, {"nameIdentifier": "40124226", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=40124226"}]}]}, "item_10001_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Two wild-type substrains of a motile cyanobacterium Synechocystis sp. PCC 6803 show positive phototaxis toward a light source (PCC-P) and negative phototaxis away from light (PCC-N). In this study, we found that a novel two-component system of photoresponse is involved in the phototactic regulation. Inactivation of slr1212 (pixA), which encodes a photoreceptor histidine kinase, reverted the positive phototaxis of PCC-P to negative phototaxis, and inactivation of the downstream slr1213 (nixB) and slr1214 (nixC), which encode AraC-like transcription factor-type and PatA-type response regulators, respectively, reverted the negative phototaxis of PCC-N to positive phototaxis. Opposite effects of pixA and nixBC disruption implies an unexpected signal transduction pathway in the switching of positive and negative phototaxis. The blue/green-type cyanobacteriochrome GAF domain of PixA was expressed in Synechocystis and phycocyanobilin-producing Escherichia coli. The holoprotein covalently bound a chromophore phycoviolobilin and showed reversible photoconversion between the violet-(Pv, λ peak = 396 nm) and green-absorbing (Pg, λ peak = 533 nm) forms, although the protein from E. coli partially bound a precursor phycocyanobilin. These results were discussed with regard to an idea that PixA serves as a violet light receptor for switching of positive and negative phototaxis by transcriptional and functional regulation.", "subitem_description_type": "Abstract"}]}, "item_10001_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Oxford University Press "}]}, "item_10001_relation_13": {"attribute_name": "PubMed番号", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "22065076", "subitem_relation_type_select": "PMID"}}]}, "item_10001_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1093/pcp/pcr155 "}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1093/pcp/pcr155", "subitem_relation_type_select": "DOI"}}]}, "item_10001_rights_15": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "© 2011 The Author"}]}, "item_10001_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "00320781 ", "subitem_source_identifier_type": "ISSN"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "NARIKAWA, Rei "}], "nameIdentifiers": [{"nameIdentifier": "1970", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "SUZUKI, Fumiko "}], "nameIdentifiers": [{"nameIdentifier": "1971", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "YOSHIHARA, Shizue "}], "nameIdentifiers": [{"nameIdentifier": "1868", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "HIGASHI, Sho-ichi"}], "nameIdentifiers": [{"nameIdentifier": "836", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "WATANABE, Masakatsu"}], "nameIdentifiers": [{"nameIdentifier": "346", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "IKEUCHI, Masahiko "}], "nameIdentifiers": [{"nameIdentifier": "1875", "nameIdentifierScheme": "WEKO"}]}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Novel photosensory two-component system (PixA-NixB-NixC) involved in the regulation of positive and negative phototaxis of cyanobacterium synechocystis sp. PCC 6803", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Novel photosensory two-component system (PixA-NixB-NixC) involved in the regulation of positive and negative phototaxis of cyanobacterium synechocystis sp. PCC 6803"}, {"subitem_title": "Novel photosensory two-component system (PixA-NixB-NixC) involved in the regulation of positive and negative phototaxis of cyanobacterium synechocystis sp. PCC 6803", "subitem_title_language": "en"}]}, "item_type_id": "10001", "owner": "21", "path": ["109", "283"], "permalink_uri": "https://ir.soken.ac.jp/records/3911", "pubdate": {"attribute_name": "公開日", "attribute_value": "2013-08-22"}, "publish_date": "2013-08-22", "publish_status": "0", "recid": "3911", "relation": {}, "relation_version_is_last": true, "title": ["Novel photosensory two-component system (PixA-NixB-NixC) involved in the regulation of positive and negative phototaxis of cyanobacterium synechocystis sp. PCC 6803"], "weko_shared_id": 21}