{"_buckets": {"deposit": "1a2ba303-3151-48ea-9f9e-804ccce89448"}, "_deposit": {"created_by": 1, "id": "1032", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "1032"}, "status": "published"}, "_oai": {"id": "oai:ir.soken.ac.jp:00001032", "sets": ["20"]}, "author_link": ["0", "0", "0"], "item_1_biblio_info_21": {"attribute_name": "書誌情報（ソート用）", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2007-03-23", "bibliographicIssueDateType": "Issued"}, "bibliographic_titles": [{}]}]}, "item_1_creator_2": {"attribute_name": "著者名", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "兼崎, 琢麿"}], "nameIdentifiers": [{"nameIdentifier": "0", "nameIdentifierScheme": "WEKO"}]}]}, "item_1_creator_3": {"attribute_name": "フリガナ", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "カネサキ, タクマ"}], "nameIdentifiers": [{"nameIdentifier": "0", "nameIdentifierScheme": "WEKO"}]}]}, "item_1_date_granted_11": {"attribute_name": "学位授与年月日", "attribute_value_mlt": [{"subitem_dategranted": "2007-03-23"}]}, "item_1_degree_grantor_5": {"attribute_name": "学位授与機関", "attribute_value_mlt": [{"subitem_degreegrantor": [{"subitem_degreegrantor_name": "総合研究大学院大学"}]}]}, "item_1_degree_name_6": {"attribute_name": "学位名", "attribute_value_mlt": [{"subitem_degreename": "博士（理学）"}]}, "item_1_description_1": {"attribute_name": "ID", "attribute_value_mlt": [{"subitem_description": "2007046", "subitem_description_type": "Other"}]}, "item_1_description_12": {"attribute_name": "要旨", "attribute_value_mlt": [{"subitem_description": "Heterotrimeric G protein signaling is involved in various pathways in animal \u003cbr /\u003edevelopment. During Drosophila gastrula ion, Ga12 (Cta)mediated signaling \u003cbr /\u003econtrols the ventral cellular movements of epithelia. Here, I show that G Protein \u003cbr /\u003esignaling also regulates the organization of cortical actin cytoskeleton underlining \u003cbr /\u003eplasma membrane in the epithelial cells. Multiple Gα subfamilies, Gα12 and \u003cbr /\u003eGαi, are involved in the actin organization, but a known ligand of Gα12, Fog, is \u003cbr /\u003enot required. When rie-8, which is required for plasma membrane-targeting of \u003cbr /\u003ewhole G proteins and their signaling activity, is mutated, the cell surfaces become \u003cbr /\u003eunstable and show blebs, and entire cellular movements in gastrulation are \u003cbr /\u003edelayed. The mutants for the common cofactors of the Gα\u0027s, GB13F and Gγ1, \u003cbr /\u003eshow the similar phenotypes. Ventral cells also showed blebs like the riｃ-8 \u003cbr /\u003emutant when the cells are treated with inhibitors of actin polymerization. The \u003cbr /\u003edisruption of cortical actin in the mutants for G proteins is seen in all epithelial \u003cbr /\u003ecells, but the disorganization of the surface occurs only in the moving ventral cells. \u003cbr /\u003eThis suggests that the organization of cortical actin seems to be required to \u003cbr /\u003eendure the stress, which the ventral cells receive when they move during \u003cbr /\u003egastrulation. \u003cbr /\u003e   Besides, I also show evidence that heterotrimeric G protein signaling is \u003cbr /\u003einvolved in the control of the actin dynamics in syncytial blastderm stage. In \u003cbr /\u003ethis stage, nuclear division is carried out with the assistance of two architectures \u003cbr /\u003eof actin cytoskeleton; actin caps in interphase and pseudocleavage \u003cbr /\u003efurrow-assembled actin network in mitosis. G protein is colocalized with the caps \u003cbr /\u003eand network of actin throughout this stage. In the ric-8mutant, this localization \u003cbr /\u003epattern is lost and actin cytoskeleton shows abnormal morphology. Using \u003cbr /\u003elive-imaging technique, it is revealed that the ric-8 mutant shows the delay of the \u003cbr /\u003echange of actin morphology. These results suggest that G protein regulates \u003cbr /\u003etiming of the dynamic change of actin during mitosis, and provide new insight \u003cbr /\u003einto the role of G protein signaling in early embryogenesis of Drosophi7a. \u003cbr /\u003e   In addition, here I introduce a novel mutant of ric-8 gene, ric8\u003csup\u003eatx\u003c/sup\u003e, in which \u003cbr /\u003e408th E is substituted to K. The ric8\u003csup\u003eatx\u003c/sup\u003e mutant embryo shows cta- or fog like \u003cbr /\u003ephenotype of gastrulation, which is milder than that of the ric-8 null mutant. \u003cbr /\u003eConsistently, the ric8\u003csup\u003eatx\u003c/sup\u003e mutation results in the Gα12-specific defect of targeting. \u003cbr /\u003eAlthough the role of Ric-8 has been inferred from the results of genetic analyses \u003cbr /\u003eand of some in vitro assays in past studies, the true molecular function of this \u003cbr /\u003eprotein is stil1 unclear. The findings from the analyses of ric8\u003csup\u003eatx\u003c/sup\u003e promote \u003cbr /\u003eelucidating the mysterious function of Ric-8 protein in viva .", "subitem_description_type": "Other"}]}, "item_1_description_7": {"attribute_name": "学位記番号", "attribute_value_mlt": [{"subitem_description": "総研大甲第1061号", "subitem_description_type": "Other"}]}, "item_1_select_14": {"attribute_name": "所蔵", "attribute_value_mlt": [{"subitem_select_item": "有"}]}, "item_1_select_8": {"attribute_name": "研究科", "attribute_value_mlt": [{"subitem_select_item": "生命科学研究科"}]}, "item_1_select_9": {"attribute_name": "専攻", "attribute_value_mlt": [{"subitem_select_item": "18 遺伝学専攻"}]}, "item_1_text_10": {"attribute_name": "学位授与年度", "attribute_value_mlt": [{"subitem_text_value": "2006"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "KANESAKI, Takuma", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "0", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-02-17"}], "displaytype": "simple", "download_preview_message": "", "file_order": 0, "filename": "甲1061_要旨.pdf", "filesize": [{"value": "279.3 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 279300.0, "url": {"label": "要旨・審査要旨", "url": "https://ir.soken.ac.jp/record/1032/files/甲1061_要旨.pdf"}, "version_id": "5c11c9b1-b2a3-4170-a4dd-8657a15a5eff"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "thesis", "resourceuri": "http://purl.org/coar/resource_type/c_46ec"}]}, "item_title": "Roles of Ric-8 dependent targeting of heterotrimeric G proteins in early embryogenesis of Drosophila", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Roles of Ric-8 dependent targeting of heterotrimeric G proteins in early embryogenesis of Drosophila"}, {"subitem_title": "Roles of Ric-8 dependent targeting of heterotrimeric G proteins in early embryogenesis of Drosophila", "subitem_title_language": "en"}]}, "item_type_id": "1", "owner": "1", "path": ["20"], "permalink_uri": "https://ir.soken.ac.jp/records/1032", "pubdate": {"attribute_name": "公開日", "attribute_value": "2010-02-22"}, "publish_date": "2010-02-22", "publish_status": "0", "recid": "1032", "relation": {}, "relation_version_is_last": true, "title": ["Roles of Ric-8 dependent targeting of heterotrimeric G proteins in early embryogenesis of Drosophila"], "weko_shared_id": -1}