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Chromatin‐Immunoprecipitation‐Mediated Target Identification Proved Aquaporin 5 is Regulated Directly by Estrogen in the Mouse Uterus
https://ir.soken.ac.jp/records/1054
https://ir.soken.ac.jp/records/1054d28c866c-d982-457d-a68a-efe80622c4c8
| 名前 / ファイル | ライセンス | アクション |
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要旨・審査要旨 (286.3 kB)
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本文 (4.4 MB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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| 公開日 | 2010-02-22 | |||||
| タイトル | ||||||
| タイトル | Chromatin‐Immunoprecipitation‐Mediated Target Identification Proved Aquaporin 5 is Regulated Directly by Estrogen in the Mouse Uterus | |||||
| タイトル | ||||||
| タイトル | Chromatin‐Immunoprecipitation‐Mediated Target Identification Proved Aquaporin 6 is Regulated Directly by Estrogen in the Mouse Uterus | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_46ec | |||||
| 資源タイプ | thesis | |||||
| 著者名 |
小林, 未佳
× 小林, 未佳 |
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| フリガナ |
コバヤシ, ミカ
× コバヤシ, ミカ |
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| 著者 |
KOBAYASHI, Mika
× KOBAYASHI, Mika |
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| 学位授与機関 | ||||||
| 学位授与機関名 | 総合研究大学院大学 | |||||
| 学位名 | ||||||
| 学位名 | 博士（理学） | |||||
| 学位記番号 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 総研大甲第1006号 | |||||
| 研究科 | ||||||
| 値 | 生命科学研究科 | |||||
| 専攻 | ||||||
| 値 | 19 基礎生物学専攻 | |||||
| 学位授与年月日 | ||||||
| 学位授与年月日 | 2006-09-29 | |||||
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| 値 | 2006 | |||||
| 要旨 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Estrogens play a central role in the reproduction of vertebrates and affect a variety of biological processes. The major target molecules of estrogens are nuclear estrogen receptors (ERs), which have been studied extensively at the molecular level. However, it remains unclear which genes are regulated directly by ERs, especially at the level of the whole organism rather than cultured cells. To identify the genes that are regulated directly by ERs in vivo, I subjected estrogen-treated mouse uteri to a modified chromatin immunoprecipitation (ChIP)-mediated target cloning strategy (Chapter 1).<br /> Sequence analysis of one of the anti-ERα antibodyprecipitated DNA fragments and alignment with the mouse genomic sequence revealed that the promoter region of the gene encoding aquaporin 5 (AQP5) contains an estrogen response element (ERE)(Chapter 2). Quantitative polymerase chain reaction (PCR) and DNA microarray analyses confirmed that AQP5 is activated soon after the administration of estrogen. Reporter assays also revealed that the 4 ERE in the promoter region of AQP5 is functional and is activated directly by estrogen. In contrast, while AQP8 is also expressed in the estrogen-treated uterus, reporter assays failed to detect a functional ERE. None of the other AQP genes responded to estrogen. The ChIP-mediated target identification strategy employed in this study may be useful for the study of other transcription factor networks. | |||||
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| 値 | 有 | |||||
