WEKO3
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Furthermore, by adding the nuclear localization signal to mbH, they obtained L transfectants (LmbHN) expressing the stabilized β catenin predominantly in the nucleus. Under the long-term aggregation conditions, LmbHN formed compact cell aggregates consisting mainly of 2-5 cells. LmbH also showed a similar aggregation activity but to a lesser extent, and parent L cells mostly remained to be dissociated. Close analyses by immunofluorescence microscopy and the cell adhesion inhibition assay with RGD peptides revealed that aggregation activity is attributed to the integrin-based cell adhesion. Considering that the amount of stabilized β catenin in the nucleus is significantly larger in LmbHN than in LmbH, they speculate that the accumulation of β catenin in the nucleus resulted in the activation of integrin-based cell adhesion. The transfectants established in this study, thus, will provide an advantageous system to selectively analyze the role of β catenin in the nucleus and in the regulation of integrin activity. 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Activation of Integrin-based Cell Adhesion inMouse L Fibroblasts Expressing Stabilized βCatenin in the Nucleus
https://ir.soken.ac.jp/records/1087
https://ir.soken.ac.jp/records/1087d6398e0a-87bd-4cb1-8c55-83dd21ff2cd9
名前 / ファイル | ライセンス | アクション |
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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公開日 | 2010-02-22 | |||||
タイトル | ||||||
タイトル | Activation of Integrin-based Cell Adhesion inMouse L Fibroblasts Expressing Stabilized βCatenin in the Nucleus | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Activation of Integrin-based Cell Adhesion inMouse L Fibroblasts Expressing Stabilized βCatenin in the Nucleus | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_46ec | |||||
資源タイプ | thesis | |||||
著者名 |
石原, 悟
× 石原, 悟 |
|||||
フリガナ |
イシハラ, サトル
× イシハラ, サトル |
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著者 |
ISHIHARA, Satoru
× ISHIHARA, Satoru |
|||||
学位授与機関 | ||||||
学位授与機関名 | 総合研究大学院大学 | |||||
学位名 | ||||||
学位名 | 博士(学術) | |||||
学位記番号 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 総研大甲第281号 | |||||
研究科 | ||||||
値 | 生命科学研究科 | |||||
専攻 | ||||||
値 | 20 生理科学専攻 | |||||
学位授与年月日 | ||||||
学位授与年月日 | 1997-03-24 | |||||
学位授与年度 | ||||||
1996 | ||||||
要旨 | ||||||
内容記述タイプ | Other | |||||
内容記述 | β catenin is involved not only in the cadherin-based cell adhesion system but also in the Wnt signaling pathway. In this pathway, it is supposed that β catenin is phosphorylated by glycogen synthase kinase-3β(GSK-3β) to become susceptible to degradation, that the Wnt signaling suppresses GSK-3β to increase the amount of non-phosphorylated, stable β catenin, and that the stable β catenin affects the transcription of various genes in the nucleus. To separate the role of β catenin in intracellular signaling from its role in the cadherin-based cell adhesion, they constructed a mutant β catenin(mbH) with amino acid substitutions at its putative GSK-3β phosphorylation site (GSKP sequence), and introduced it into L fibroblasts that lack the cadherin expression. In stable transfectants (LmbH), not only mbH but also endogenous wild type β catenin was stabilized. Green fluorescence protein (GFP) carrying the mutated GSKP sequence also stabilized the endogenous β catenin in L cells, indicating that the mutated GSKP sequence suppresses the degradation machinery for β catenin. Furthermore, by adding the nuclear localization signal to mbH, they obtained L transfectants (LmbHN) expressing the stabilized β catenin predominantly in the nucleus. Under the long-term aggregation conditions, LmbHN formed compact cell aggregates consisting mainly of 2-5 cells. LmbH also showed a similar aggregation activity but to a lesser extent, and parent L cells mostly remained to be dissociated. Close analyses by immunofluorescence microscopy and the cell adhesion inhibition assay with RGD peptides revealed that aggregation activity is attributed to the integrin-based cell adhesion. Considering that the amount of stabilized β catenin in the nucleus is significantly larger in LmbHN than in LmbH, they speculate that the accumulation of β catenin in the nucleus resulted in the activation of integrin-based cell adhesion. The transfectants established in this study, thus, will provide an advantageous system to selectively analyze the role of β catenin in the nucleus and in the regulation of integrin activity. | |||||
所蔵 | ||||||
値 | 有 | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf |