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  1. 020 学位論文
  2. 生命科学研究科
  3. 20 生理科学専攻

Activation of Integrin-based Cell Adhesion inMouse L Fibroblasts Expressing Stabilized βCatenin in the Nucleus

https://ir.soken.ac.jp/records/1087
https://ir.soken.ac.jp/records/1087
d6398e0a-87bd-4cb1-8c55-83dd21ff2cd9
名前 / ファイル ライセンス アクション
甲281_要旨.pdf 要旨・審査要旨 / Abstract, Screening Result (181.4 kB)
甲281_本文.pdf 本文 (4.0 MB)
Item type 学位論文 / Thesis or Dissertation(1)
公開日 2010-02-22
タイトル
タイトル Activation of Integrin-based Cell Adhesion inMouse L Fibroblasts Expressing Stabilized βCatenin in the Nucleus
タイトル
タイトル Activation of Integrin-based Cell Adhesion inMouse L Fibroblasts Expressing Stabilized βCatenin in the Nucleus
言語 en
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_46ec
資源タイプ thesis
著者名 石原, 悟

× 石原, 悟

石原, 悟

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フリガナ イシハラ, サトル

× イシハラ, サトル

イシハラ, サトル

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著者 ISHIHARA, Satoru

× ISHIHARA, Satoru

en ISHIHARA, Satoru

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学位授与機関
学位授与機関名 総合研究大学院大学
学位名
学位名 博士(学術)
学位記番号
内容記述タイプ Other
内容記述 総研大甲第281号
研究科
値 生命科学研究科
専攻
値 20 生理科学専攻
学位授与年月日
学位授与年月日 1997-03-24
学位授与年度
値 1996
要旨
内容記述タイプ Other
内容記述 β catenin is involved not only in the cadherin-based cell adhesion system but also in the Wnt signaling pathway. In this pathway, it is supposed that β catenin is phosphorylated by glycogen synthase kinase-3β(GSK-3β) to become susceptible to degradation, that the Wnt signaling suppresses GSK-3β to increase the amount of non-phosphorylated, stable β catenin, and that the stable β catenin affects the transcription of various genes in the nucleus. To separate the role of β catenin in intracellular signaling from its role in the cadherin-based cell adhesion, they constructed a mutant β catenin(mbH) with amino acid substitutions at its putative GSK-3β phosphorylation site (GSKP sequence), and introduced it into L fibroblasts that lack the cadherin expression. In stable transfectants (LmbH), not only mbH but also endogenous wild type β catenin was stabilized. Green fluorescence protein (GFP) carrying the mutated GSKP sequence also stabilized the endogenous β catenin in L cells, indicating that the mutated GSKP sequence suppresses the degradation machinery for β catenin. Furthermore, by adding the nuclear localization signal to mbH, they obtained L transfectants (LmbHN) expressing the stabilized β catenin predominantly in the nucleus. Under the long-term aggregation conditions, LmbHN formed compact cell aggregates consisting mainly of 2-5 cells. LmbH also showed a similar aggregation activity but to a lesser extent, and parent L cells mostly remained to be dissociated. Close analyses by immunofluorescence microscopy and the cell adhesion inhibition assay with RGD peptides revealed that aggregation activity is attributed to the integrin-based cell adhesion. Considering that the amount of stabilized β catenin in the nucleus is significantly larger in LmbHN than in LmbH, they speculate that the accumulation of β catenin in the nucleus resulted in the activation of integrin-based cell adhesion. The transfectants established in this study, thus, will provide an advantageous system to selectively analyze the role of β catenin in the nucleus and in the regulation of integrin activity.
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内容記述 application/pdf
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