{"_buckets": {"deposit": "6ecd5fb3-7272-4385-9313-ddfda832e408"}, "_deposit": {"created_by": 1, "id": "1110", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "1110"}, "status": "published"}, "_oai": {"id": "oai:ir.soken.ac.jp:00001110", "sets": ["22"]}, "author_link": ["9350", "9349", "9348"], "item_1_biblio_info_21": {"attribute_name": "書誌情報（ソート用）", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2000-03-24", "bibliographicIssueDateType": "Issued"}, "bibliographic_titles": [{}]}]}, "item_1_creator_2": {"attribute_name": "著者名", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "范, 海天"}], "nameIdentifiers": [{"nameIdentifier": "9348", "nameIdentifierScheme": "WEKO"}]}]}, "item_1_creator_3": {"attribute_name": "フリガナ", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "ハン, カイテン"}], "nameIdentifiers": [{"nameIdentifier": "9349", "nameIdentifierScheme": "WEKO"}]}]}, "item_1_date_granted_11": {"attribute_name": "学位授与年月日", "attribute_value_mlt": [{"subitem_dategranted": "2000-03-24"}]}, "item_1_degree_grantor_5": {"attribute_name": "学位授与機関", "attribute_value_mlt": [{"subitem_degreegrantor": [{"subitem_degreegrantor_name": "総合研究大学院大学"}]}]}, "item_1_degree_name_6": {"attribute_name": "学位名", "attribute_value_mlt": [{"subitem_degreename": "博士（理学）"}]}, "item_1_description_1": {"attribute_name": "ID", "attribute_value_mlt": [{"subitem_description": "2000043", "subitem_description_type": "Other"}]}, "item_1_description_12": {"attribute_name": "要旨", "attribute_value_mlt": [{"subitem_description": "Phloretin, which is the aglucon of phloridzin, has been widely used as an inhibitor of glucose transporter. Since we have recently found that phloretin inhibits a regulatory volume decrease after osmotic swelling in a human epithelial cell line, in the present study, I investigated the effects of phloretin on volume-sensitive Cl- channels using three different epithelial cell lines by the whole-cell patch-clamp technique. Extracellular application of phloretin (over 10 μM) was found to inhibit, in a concentration-dependent manner (IC50～20 μM), the volume-sensitive Cl- current activated by a hypotonic challenge in human epithelial T84 and Intestine 407 cells as well as in mouse epithelial C127/CFTR cells. In contrast, at a low concentration (30 μM), phloretin failed to inhibit CFTR Cl- currents activated by cAMP stimulation in T84 and C127/CFTR cells. At a high concentration (300 μM), however, partial inhibition by phloretin was observed in the CFTR Cl- currents in both cell types. At 30 and 300 μM, on the other hand, phloretin showed no inhibitory effect on Ca2+- activated Cl- currents induced by ionomycin in human T84 cells but rather reactivated them after inactivation during stimulation with ionomycin. It is concluded that phloretin is a novel inhibitor of volume-sensitive Cl- channel, and that at low concentrations phloretin specifically blocks this channel without inhibiting both cAMP-activated and Ca2+activated Cl- channels in epithelial cells.", "subitem_description_type": "Other"}]}, "item_1_description_18": {"attribute_name": "フォーマット", "attribute_value_mlt": [{"subitem_description": "application/pdf", "subitem_description_type": "Other"}]}, "item_1_description_7": {"attribute_name": "学位記番号", "attribute_value_mlt": [{"subitem_description": "総研大甲第479号", "subitem_description_type": "Other"}]}, "item_1_select_14": {"attribute_name": "所蔵", "attribute_value_mlt": [{"subitem_select_item": "有"}]}, "item_1_select_8": {"attribute_name": "研究科", "attribute_value_mlt": [{"subitem_select_item": "生命科学研究科"}]}, "item_1_select_9": {"attribute_name": "専攻", "attribute_value_mlt": [{"subitem_select_item": "20 生理科学専攻"}]}, "item_1_text_10": {"attribute_name": "学位授与年度", "attribute_value_mlt": [{"subitem_text_value": "1999"}]}, "item_1_text_20": {"attribute_name": "業務メモ", "attribute_value_mlt": [{"subitem_text_value": "（2018年2月15日）本籍など個人情報の記載がある旧要旨・審査要旨を個人情報のない新しいものに差し替えた。承諾書等未確認。要確認該当項目修正のこと。"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "FAN,  Hai-Tian", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "9350", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-02-17"}], "displaytype": "simple", "download_preview_message": "", "file_order": 0, "filename": "甲479_要旨.pdf", "filesize": [{"value": "190.0 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 190000.0, "url": {"label": "要旨・審査要旨 / Abstract, Screening Result", "url": "https://ir.soken.ac.jp/record/1110/files/甲479_要旨.pdf"}, "version_id": "461b3219-826a-4ea9-90fb-5c16b8af1034"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-02-17"}], "displaytype": "simple", "download_preview_message": "", "file_order": 1, "filename": "甲479_本文.pdf", "filesize": [{"value": "1.2 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 1200000.0, "url": {"label": "本文", "url": "https://ir.soken.ac.jp/record/1110/files/甲479_本文.pdf"}, "version_id": "f680c3d4-09ec-47c4-9f47-43640e2a310c"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "thesis", "resourceuri": "http://purl.org/coar/resource_type/c_46ec"}]}, "item_title": "Effects of Phloretin on Three Types of Chloride Channels in Epithelial Cells", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Effects of Phloretin on Three Types of Chloride Channels in Epithelial Cells"}, {"subitem_title": "Effects of Phloretin on Three Types of Chloride Channels in Epithelial Cells", "subitem_title_language": "en"}]}, "item_type_id": "1", "owner": "1", "path": ["22"], "permalink_uri": "https://ir.soken.ac.jp/records/1110", "pubdate": {"attribute_name": "公開日", "attribute_value": "2010-02-22"}, "publish_date": "2010-02-22", "publish_status": "0", "recid": "1110", "relation": {}, "relation_version_is_last": true, "title": ["Effects of Phloretin on Three Types of Chloride Channels in Epithelial Cells"], "weko_shared_id": 1}