| Item type |
学位論文 / Thesis or Dissertation(1) |
| 公開日 |
2010-02-22 |
| タイトル |
|
|
タイトル |
Effects of Phloretin on Three Types of Chloride Channels in Epithelial Cells |
| タイトル |
|
|
タイトル |
Effects of Phloretin on Three Types of Chloride Channels in Epithelial Cells |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_46ec |
|
資源タイプ |
thesis |
| 著者名 |
范, 海天
|
| フリガナ |
ハン, カイテン
|
| 著者 |
FAN, Hai-Tian
|
| 学位授与機関 |
|
|
|
学位授与機関名 |
総合研究大学院大学 |
| 学位名 |
|
|
学位名 |
博士（理学） |
| 学位記番号 |
|
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内容記述タイプ |
Other |
|
内容記述 |
総研大甲第479号 |
| 研究科 |
|
|
値 |
生命科学研究科 |
| 専攻 |
|
|
値 |
20 生理科学専攻 |
| 学位授与年月日 |
|
|
学位授与年月日 |
2000-03-24 |
| 学位授与年度 |
|
|
値 |
1999 |
| 要旨 |
|
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内容記述タイプ |
Other |
|
内容記述 |
Phloretin, which is the aglucon of phloridzin, has been widely used as an inhibitor of glucose transporter. Since we have recently found that phloretin inhibits a regulatory volume decrease after osmotic swelling in a human epithelial cell line, in the present study, I investigated the effects of phloretin on volume-sensitive Cl- channels using three different epithelial cell lines by the whole-cell patch-clamp technique. Extracellular application of phloretin (over 10 μM) was found to inhibit, in a concentration-dependent manner (IC50～20 μM), the volume-sensitive Cl- current activated by a hypotonic challenge in human epithelial T84 and Intestine 407 cells as well as in mouse epithelial C127/CFTR cells. In contrast, at a low concentration (30 μM), phloretin failed to inhibit CFTR Cl- currents activated by cAMP stimulation in T84 and C127/CFTR cells. At a high concentration (300 μM), however, partial inhibition by phloretin was observed in the CFTR Cl- currents in both cell types. At 30 and 300 μM, on the other hand, phloretin showed no inhibitory effect on Ca2+- activated Cl- currents induced by ionomycin in human T84 cells but rather reactivated them after inactivation during stimulation with ionomycin. It is concluded that phloretin is a novel inhibitor of volume-sensitive Cl- channel, and that at low concentrations phloretin specifically blocks this channel without inhibiting both cAMP-activated and Ca2+activated Cl- channels in epithelial cells. |
| 所蔵 |
|
|
値 |
有 |
| フォーマット |
|
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内容記述タイプ |
Other |
|
内容記述 |
application/pdf |
要旨・審査要旨 / Abstract, Screening Result (190.0 kB)
