Item type |
学位論文 / Thesis or Dissertation(1) |
公開日 |
2010-02-22 |
タイトル |
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タイトル |
Effects of Phloretin on Three Types of Chloride Channels in Epithelial Cells |
タイトル |
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タイトル |
Effects of Phloretin on Three Types of Chloride Channels in Epithelial Cells |
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言語 |
en |
言語 |
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言語 |
eng |
資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_46ec |
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資源タイプ |
thesis |
著者名 |
范, 海天
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フリガナ |
ハン, カイテン
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著者 |
FAN, Hai-Tian
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学位授与機関 |
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学位授与機関名 |
総合研究大学院大学 |
学位名 |
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学位名 |
博士(理学) |
学位記番号 |
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内容記述タイプ |
Other |
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内容記述 |
総研大甲第479号 |
研究科 |
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値 |
生命科学研究科 |
専攻 |
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値 |
20 生理科学専攻 |
学位授与年月日 |
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学位授与年月日 |
2000-03-24 |
学位授与年度 |
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値 |
1999 |
要旨 |
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内容記述タイプ |
Other |
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内容記述 |
Phloretin, which is the aglucon of phloridzin, has been widely used as an inhibitor of glucose transporter. Since we have recently found that phloretin inhibits a regulatory volume decrease after osmotic swelling in a human epithelial cell line, in the present study, I investigated the effects of phloretin on volume-sensitive Cl- channels using three different epithelial cell lines by the whole-cell patch-clamp technique. Extracellular application of phloretin (over 10 μM) was found to inhibit, in a concentration-dependent manner (IC50~20 μM), the volume-sensitive Cl- current activated by a hypotonic challenge in human epithelial T84 and Intestine 407 cells as well as in mouse epithelial C127/CFTR cells. In contrast, at a low concentration (30 μM), phloretin failed to inhibit CFTR Cl- currents activated by cAMP stimulation in T84 and C127/CFTR cells. At a high concentration (300 μM), however, partial inhibition by phloretin was observed in the CFTR Cl- currents in both cell types. At 30 and 300 μM, on the other hand, phloretin showed no inhibitory effect on Ca2+- activated Cl- currents induced by ionomycin in human T84 cells but rather reactivated them after inactivation during stimulation with ionomycin. It is concluded that phloretin is a novel inhibitor of volume-sensitive Cl- channel, and that at low concentrations phloretin specifically blocks this channel without inhibiting both cAMP-activated and Ca2+activated Cl- channels in epithelial cells. |
所蔵 |
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値 |
有 |
フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |