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Effects of Phloretin on Three Types of Chloride Channels in Epithelial Cells
https://ir.soken.ac.jp/records/1110
https://ir.soken.ac.jp/records/1110b7cd7f79-4c17-45e8-b58f-c700b21611e2
| 名前 / ファイル | ライセンス | アクション |
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要旨・審査要旨 / Abstract, Screening Result (190.0 kB)
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本文 (1.2 MB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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| 公開日 | 2010-02-22 | |||||
| タイトル | ||||||
| タイトル | Effects of Phloretin on Three Types of Chloride Channels in Epithelial Cells | |||||
| タイトル | ||||||
| タイトル | Effects of Phloretin on Three Types of Chloride Channels in Epithelial Cells | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_46ec | |||||
| 資源タイプ | thesis | |||||
| 著者名 |
范, 海天
× 范, 海天 |
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| フリガナ |
ハン, カイテン
× ハン, カイテン |
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| 著者 |
FAN, Hai-Tian
× FAN, Hai-Tian |
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| 学位授与機関 | ||||||
| 学位授与機関名 | 総合研究大学院大学 | |||||
| 学位名 | ||||||
| 学位名 | 博士（理学） | |||||
| 学位記番号 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 総研大甲第479号 | |||||
| 研究科 | ||||||
| 値 | 生命科学研究科 | |||||
| 専攻 | ||||||
| 値 | 20 生理科学専攻 | |||||
| 学位授与年月日 | ||||||
| 学位授与年月日 | 2000-03-24 | |||||
| 学位授与年度 | ||||||
| 値 | 1999 | |||||
| 要旨 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Phloretin, which is the aglucon of phloridzin, has been widely used as an inhibitor of glucose transporter. Since we have recently found that phloretin inhibits a regulatory volume decrease after osmotic swelling in a human epithelial cell line, in the present study, I investigated the effects of phloretin on volume-sensitive Cl- channels using three different epithelial cell lines by the whole-cell patch-clamp technique. Extracellular application of phloretin (over 10 μM) was found to inhibit, in a concentration-dependent manner (IC50～20 μM), the volume-sensitive Cl- current activated by a hypotonic challenge in human epithelial T84 and Intestine 407 cells as well as in mouse epithelial C127/CFTR cells. In contrast, at a low concentration (30 μM), phloretin failed to inhibit CFTR Cl- currents activated by cAMP stimulation in T84 and C127/CFTR cells. At a high concentration (300 μM), however, partial inhibition by phloretin was observed in the CFTR Cl- currents in both cell types. At 30 and 300 μM, on the other hand, phloretin showed no inhibitory effect on Ca2+- activated Cl- currents induced by ionomycin in human T84 cells but rather reactivated them after inactivation during stimulation with ionomycin. It is concluded that phloretin is a novel inhibitor of volume-sensitive Cl- channel, and that at low concentrations phloretin specifically blocks this channel without inhibiting both cAMP-activated and Ca2+activated Cl- channels in epithelial cells. | |||||
| 所蔵 | ||||||
| 値 | 有 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
