| Item type |
学位論文 / Thesis or Dissertation(1) |
| 公開日 |
2010-02-22 |
| タイトル |
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|
タイトル |
Physiological and morphological studies on cerebellar synapses of CaV2.1 mutant mice rocker |
| タイトル |
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|
タイトル |
Physiological and morphological studies on cerebellar synapses of CaV2.2 mutant mice rocker |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_46ec |
|
資源タイプ |
thesis |
| 著者名 |
児玉, 貴史
|
| フリガナ |
コダマ, タカシ
|
| 著者 |
KODAMA, Takashi
|
| 学位授与機関 |
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学位授与機関名 |
総合研究大学院大学 |
| 学位名 |
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学位名 |
博士（理学） |
| 学位記番号 |
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内容記述タイプ |
Other |
|
内容記述 |
総研大甲第1010号 |
| 研究科 |
|
|
値 |
生命科学研究科 |
| 専攻 |
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|
値 |
20 生理科学専攻 |
| 学位授与年月日 |
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学位授与年月日 |
2006-09-29 |
| 学位授与年度 |
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値 |
2006 |
| 要旨 |
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内容記述タイプ |
Other |
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内容記述 |
The rocker mice are hereditary ataxic mutants, which carry a point mutation in the gene<br />encoding the Ca<sub>V</sub> 2.1 (P/Q-type) Ca<sup>2+</sup> channel α1 subunit. They show the mildest<br />phenotypes among the reported Ca<sub>V</sub> 2.1 mutant mice, allowing us to evaluate primary<br />symptoms caused by the Ca<sub>V</sub> 2.1 dysfunction. Although the basic properties of the<br />rocker mutant channel have been reported, their influence on physiological functions<br />remains unclear. Here, I studied the impacts of the rocker mutant Ca<sup>2+</sup> channel on<br />synaptic transmission especially in cerebellar Purkinje cells, where Ca<sub>V</sub> 2.1 Ca<sup>2+</sup> channel<br />mediates a major activity-dependent Ca<sup>2+</sup> influx. Despite of the small changes in<br />channel function, synaptic transmission in the parallel fiber (PF)-Purkinje cell (PC)<br />synapses was severely impaired, whereas the climbing fiber inputs onto PCs appeared to function normally. I further explored the origin of the transmission impairment in the<br />PF-PC synapses in rocker mice. Presynaptic functions of the PF-PC synapses, however,<br />were unexpectedly normal in terms of paired-pulse facilitation, sensitivity to<br />extracellular Ca<sup>2+</sup> concentration, and glutamate concentration in synaptic clefts.<br />Electron microscopic analyses including freeze-fracture replica labeling revealed that<br />both the number and density of postsynaptic AMPA<br />(α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid) receptors substantially<br />decreased without gross structural changes of the PF-PC synapses. I also observed an<br />abnormal arborization of PC dendrites in young adult rocker mice (「1 month of age).<br />These lines of evidence suggest that the rocker mutation exerts its influence especially<br />on postsynaptic structures and functions in the PF-PC synapses. |
| 所蔵 |
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値 |
有 |
要旨・審査要旨 (195.8 kB)
