{"_buckets": {"deposit": "4b44ed9d-ab12-493f-8853-4b862a1a2432"}, "_deposit": {"created_by": 1, "id": "1181", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "1181"}, "status": "published"}, "_oai": {"id": "oai:ir.soken.ac.jp:00001181", "sets": ["22"]}, "author_link": ["0", "0", "0"], "item_1_biblio_info_21": {"attribute_name": "書誌情報（ソート用）", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2006-09-29", "bibliographicIssueDateType": "Issued"}, "bibliographic_titles": [{}]}]}, "item_1_creator_2": {"attribute_name": "著者名", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "児玉, 貴史"}], "nameIdentifiers": [{"nameIdentifier": "0", "nameIdentifierScheme": "WEKO"}]}]}, "item_1_creator_3": {"attribute_name": "フリガナ", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "コダマ, タカシ"}], "nameIdentifiers": [{"nameIdentifier": "0", "nameIdentifierScheme": "WEKO"}]}]}, "item_1_date_granted_11": {"attribute_name": "学位授与年月日", "attribute_value_mlt": [{"subitem_dategranted": "2006-09-29"}]}, "item_1_degree_grantor_5": {"attribute_name": "学位授与機関", "attribute_value_mlt": [{"subitem_degreegrantor": [{"subitem_degreegrantor_name": "総合研究大学院大学"}]}]}, "item_1_degree_name_6": {"attribute_name": "学位名", "attribute_value_mlt": [{"subitem_degreename": "博士（理学）"}]}, "item_1_description_1": {"attribute_name": "ID", "attribute_value_mlt": [{"subitem_description": "2006530", "subitem_description_type": "Other"}]}, "item_1_description_12": {"attribute_name": "要旨", "attribute_value_mlt": [{"subitem_description": "The rocker mice are hereditary ataxic mutants, which carry a point mutation in the gene\u003cbr /\u003eencoding the Ca\u003csub\u003eV\u003c/sub\u003e 2.1 (P/Q-type) Ca\u003csup\u003e2+\u003c/sup\u003e channel α1 subunit. They show the mildest\u003cbr /\u003ephenotypes among the reported Ca\u003csub\u003eV\u003c/sub\u003e 2.1 mutant mice, allowing us to evaluate primary\u003cbr /\u003esymptoms caused by the Ca\u003csub\u003eV\u003c/sub\u003e 2.1 dysfunction. Although the basic properties of the\u003cbr /\u003erocker mutant channel have been reported, their influence on physiological functions\u003cbr /\u003eremains unclear. Here, I studied the impacts of the rocker mutant Ca\u003csup\u003e2+\u003c/sup\u003e channel on\u003cbr /\u003esynaptic transmission especially in cerebellar Purkinje cells, where Ca\u003csub\u003eV\u003c/sub\u003e 2.1 Ca\u003csup\u003e2+\u003c/sup\u003e channel\u003cbr /\u003emediates a major activity-dependent Ca\u003csup\u003e2+\u003c/sup\u003e influx. Despite of the small changes in\u003cbr /\u003echannel function, synaptic transmission in the parallel fiber (PF)-Purkinje cell (PC)\u003cbr /\u003esynapses was severely impaired, whereas the climbing fiber inputs onto PCs appeared to function normally. I further explored the origin of the transmission impairment in the\u003cbr /\u003ePF-PC synapses in rocker mice. Presynaptic functions of the PF-PC synapses, however,\u003cbr /\u003ewere unexpectedly normal in terms of paired-pulse facilitation, sensitivity to\u003cbr /\u003eextracellular Ca\u003csup\u003e2+\u003c/sup\u003e concentration, and glutamate concentration in synaptic clefts.\u003cbr /\u003eElectron microscopic analyses including freeze-fracture replica labeling revealed that\u003cbr /\u003eboth  the  number  and  density  of  postsynaptic  AMPA\u003cbr /\u003e(α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid) receptors substantially\u003cbr /\u003edecreased without gross structural changes of the PF-PC synapses. I also observed an\u003cbr /\u003eabnormal arborization of PC dendrites in young adult rocker mice (「1 month of age).\u003cbr /\u003eThese lines of evidence suggest that the rocker mutation exerts its influence especially\u003cbr /\u003eon postsynaptic structures and functions in the PF-PC synapses.", "subitem_description_type": "Other"}]}, "item_1_description_7": {"attribute_name": "学位記番号", "attribute_value_mlt": [{"subitem_description": "総研大甲第1010号", "subitem_description_type": "Other"}]}, "item_1_select_14": {"attribute_name": "所蔵", "attribute_value_mlt": [{"subitem_select_item": "有"}]}, "item_1_select_16": {"attribute_name": "複写", "attribute_value_mlt": [{"subitem_select_item": "application/pdf"}]}, "item_1_select_8": {"attribute_name": "研究科", "attribute_value_mlt": [{"subitem_select_item": "生命科学研究科"}]}, "item_1_select_9": {"attribute_name": "専攻", "attribute_value_mlt": [{"subitem_select_item": "20 生理科学専攻"}]}, "item_1_text_10": {"attribute_name": "学位授与年度", "attribute_value_mlt": [{"subitem_text_value": "2006"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "KODAMA, Takashi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "0", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-02-17"}], "displaytype": "simple", "download_preview_message": "", "file_order": 0, "filename": "甲1010_要旨.pdf", "filesize": [{"value": "195.8 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 195800.0, "url": {"label": "要旨・審査要旨", "url": "https://ir.soken.ac.jp/record/1181/files/甲1010_要旨.pdf"}, "version_id": "380c5751-46d9-4be5-a4d3-ef5319961443"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-02-17"}], "displaytype": "simple", "download_preview_message": "", "file_order": 1, "filename": "甲1010_本文.pdf", "filesize": [{"value": "19.7 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 19700000.0, "url": {"label": "本文", "url": "https://ir.soken.ac.jp/record/1181/files/甲1010_本文.pdf"}, "version_id": "3fba4a9d-f761-4b47-8a54-329b7febf8b7"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "thesis", "resourceuri": "http://purl.org/coar/resource_type/c_46ec"}]}, "item_title": "Physiological and morphological studies on cerebellar synapses of CaV2.1 mutant mice rocker", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Physiological and morphological studies on cerebellar synapses of CaV2.1 mutant mice rocker"}, {"subitem_title": "Physiological and morphological studies on cerebellar synapses of CaV2.2 mutant mice rocker", "subitem_title_language": "en"}]}, "item_type_id": "1", "owner": "1", "path": ["22"], "permalink_uri": "https://ir.soken.ac.jp/records/1181", "pubdate": {"attribute_name": "公開日", "attribute_value": "2010-02-22"}, "publish_date": "2010-02-22", "publish_status": "0", "recid": "1181", "relation": {}, "relation_version_is_last": true, "title": ["Physiological and morphological studies on cerebellar synapses of CaV2.1 mutant mice rocker"], "weko_shared_id": 1}