{"_buckets": {"deposit": "38ce16a7-769d-45c1-bae0-8a4936179d95"}, "_deposit": {"created_by": 1, "id": "1338", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "1338"}, "status": "published"}, "_oai": {"id": "oai:ir.soken.ac.jp:00001338", "sets": ["27"]}, "author_link": ["9497", "9495", "9496"], "item_1_biblio_info_21": {"attribute_name": "書誌情報（ソート用）", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1999-09-30", "bibliographicIssueDateType": "Issued"}, "bibliographic_titles": [{}]}]}, "item_1_creator_2": {"attribute_name": "著者名", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "森田, 清和"}], "nameIdentifiers": [{"nameIdentifier": "9495", "nameIdentifierScheme": "WEKO"}]}]}, "item_1_creator_3": {"attribute_name": "フリガナ", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "モリタ, キヨカズ"}], "nameIdentifiers": [{"nameIdentifier": "9496", "nameIdentifierScheme": "WEKO"}]}]}, "item_1_date_granted_11": {"attribute_name": "学位授与年月日", "attribute_value_mlt": [{"subitem_dategranted": "1999-09-30"}]}, "item_1_degree_grantor_5": {"attribute_name": "学位授与機関", "attribute_value_mlt": [{"subitem_degreegrantor": [{"subitem_degreegrantor_name": "総合研究大学院大学"}]}]}, "item_1_degree_name_6": {"attribute_name": "学位名", "attribute_value_mlt": [{"subitem_degreename": "博士（理学）"}]}, "item_1_description_1": {"attribute_name": "ID", "attribute_value_mlt": [{"subitem_description": "1999814", "subitem_description_type": "Other"}]}, "item_1_description_12": {"attribute_name": "要旨", "attribute_value_mlt": [{"subitem_description": "  He has identified a new member of the TGF-β superfamily, CET-1, from Caenorhabditis elegans.  cet-1::GFP fusion is expressed in the ventral nerve cord and other neurons.  He isolated cet-1 null mutant by Tc1 deletion method.  cet-1 nullmutants have shortened bodies and male tail abnormal phenotype resembling sma mutants.  These results suggest that cet-1, sma-2, sma-3, and sma-4 share a common  pathway.  Overexpression experiments demonstrated that cet-1 function requires wild type sma genes.  Interestingly, CET-1 appears to affect body length in a dose dependent manner.  Heterozygotes for cet-1 displayed body lengths ranging between null mutant and wild type, and overexpression of CET-1 in wild type worms elongated body length close to Jon mutants.  Furthermore, his results show that cet-1 controls body length not proportionally but in two particular regions of the worm probably by changing cell volume.  Moreover, genetic interaction of the cet-1 with mutants of the other TGF- β pathway in C. elegans, daf demonstrated that cet-1 is also involved at least partly in the daf signaling pathway.  In male sensory ray patterning, lack of cet-1 function results in ray fusions.  Epistasis analysis revealed that mab-21 lies downstream and is negatively regulated by the cet-1/sma pathway in the male tail.  His results show that cet-1 controls diversed biological processes during C. elegans development probably through different target genes.\u003cbr /\u003e  He also has identified two genes, CeBRAM-1A and CeBRAM-2B, which are similar to human BRAM-1 previously identified as a BMP receptor associated molecule, in C. elegans.  The C. elegans BRAMs (CeBRAM-1A and CeBRAM-2B) show significant amino acid identity with human BRAM-1, particularly in the C-terminal region.   CeBRAM-1A was found to associate with DAF-1, the type I receptor in the daf pathway in C elegans as well as a vertebrate BMP type I receptor BMPRIA.  CeBRAM-1A::GFP fusion protein is expressed mainly in amphid neurons such as ASK, ASH, and ASG etc, where DAF-1 is expressed. Loss-of-function of CeBRAM-1A gene showed that the mutant worms looked normal except that they showed a behavior of head-lifting phenotype.  Genetic interaction of CeBRAM-1A with previously known daf-7 TGF-β pathway mutants revealed that CeBRAM-1A negatively regulates the daf-7 TGF-β pathway and functions between daf-1 type I receptor and daf-14 SMAD.  On the other hand, CeBRAM-2B::GFP fusion protein was expressed strongly in pharyngeal muscle and intestinal cells. This expression pattern of CeBRAM-2B is similar to that of sma-6 type I receptor of the cet-1/sma pathway. Interestingly, double strand RNA interference (dsRNAi) of CeBR,AM-2B showed Lon phenotype.  These results strongly suggest that CeBRAM-1A and CeBRAM-2B may function as negative regulators of two distinct TGF-β signal pathways, the daf pathway and the sma pathway respectively in C. elegans.  Thus, he proposes that CeBRAM-1A and CeBRAM-2B define a novel class of molecules that serve as a negative regulator of TGF- f3 pathways acting downstream of the type I receptor.", "subitem_description_type": "Other"}]}, "item_1_description_18": {"attribute_name": "フォーマット", "attribute_value_mlt": [{"subitem_description": "application/pdf", "subitem_description_type": "Other"}]}, "item_1_description_7": {"attribute_name": "学位記番号", "attribute_value_mlt": [{"subitem_description": "総研大乙第71号", "subitem_description_type": "Other"}]}, "item_1_select_14": {"attribute_name": "所蔵", "attribute_value_mlt": [{"subitem_select_item": "有"}]}, "item_1_select_8": {"attribute_name": "研究科", "attribute_value_mlt": [{"subitem_select_item": "生命科学研究科"}]}, "item_1_select_9": {"attribute_name": "専攻", "attribute_value_mlt": [{"subitem_select_item": "X2 分子生物機構論専攻"}]}, "item_1_text_10": {"attribute_name": "学位授与年度", "attribute_value_mlt": [{"subitem_text_value": "1999"}]}, "item_1_text_20": {"attribute_name": "業務メモ", "attribute_value_mlt": [{"subitem_text_value": "（2018年2月16日）本籍など個人情報の記載がある旧要旨・審査要旨を個人情報のない新しいものに差し替えた。承諾書等未確認。要確認該当項目修正のこと。"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "MORITA, Kiyokazu", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "9497", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-02-17"}], "displaytype": "simple", "download_preview_message": "", "file_order": 0, "filename": "乙71_要旨.pdf", "filesize": [{"value": "268.2 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 268200.0, "url": {"label": "要旨・審査要旨 / Abstract, Screening Result", "url": "https://ir.soken.ac.jp/record/1338/files/乙71_要旨.pdf"}, "version_id": "a003eec5-eeba-4cef-b590-f7ddd75012e0"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-02-17"}], "displaytype": "simple", "download_preview_message": "", "file_order": 1, "filename": "乙71_本文.pdf", "filesize": [{"value": "2.8 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 2800000.0, "url": {"label": "本文", "url": "https://ir.soken.ac.jp/record/1338/files/乙71_本文.pdf"}, "version_id": "48a2b65b-c542-4d0d-86c7-fbf58f4ca80f"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "thesis", "resourceuri": "http://purl.org/coar/resource_type/c_46ec"}]}, "item_title": "The Role of a Member of the TransformingGrowth Factor-β Family cet-1 andNew Signal Mediators CeBRAM-1A and CeBRAM-2B in the NematodeCaenorhabditis elegans", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "The Role of a Member of the TransformingGrowth Factor-β Family cet-1 andNew Signal Mediators CeBRAM-1A and CeBRAM-2B in the NematodeCaenorhabditis elegans"}, {"subitem_title": "The Role of a Member of the TransformingGrowth Factor-β Family cet-1 andNew Signal Mediators CeBRAM-1A and CeBRAM-2B in the NematodeCaenorhabditis elegans", "subitem_title_language": "en"}]}, "item_type_id": "1", "owner": "1", "path": ["27"], "permalink_uri": "https://ir.soken.ac.jp/records/1338", "pubdate": {"attribute_name": "公開日", "attribute_value": "2010-02-22"}, "publish_date": "2010-02-22", "publish_status": "0", "recid": "1338", "relation": {}, "relation_version_is_last": true, "title": ["The Role of a Member of the TransformingGrowth Factor-β Family cet-1 andNew Signal Mediators CeBRAM-1A and CeBRAM-2B in the NematodeCaenorhabditis elegans"], "weko_shared_id": 1}