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  1. 020 学位論文
  2. 生命科学研究科
  3. X2 分子生物機構論専攻

Identification and characterization of the Pr gene that is responsible for blue flower pigmentation in the Japanese morning glory

https://ir.soken.ac.jp/records/1348
https://ir.soken.ac.jp/records/1348
3e95e49d-8ae8-468d-b0e0-8f90a5aa7d6b
名前 / ファイル ライセンス アクション
甲539_要旨.pdf 要旨・審査要旨 / Abstract, Screening Result (203.4 kB)
甲539_本文.pdf 本文 (5.6 MB)
Item type 学位論文 / Thesis or Dissertation(1)
公開日 2010-02-22
タイトル
タイトル Identification and characterization of the Pr gene that is responsible for blue flower pigmentation in the Japanese morning glory
タイトル
タイトル Identification and characterization of the Pr gene that is responsible for blue flower pigmentation in the Japanese morning glory
言語 en
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_46ec
資源タイプ thesis
著者名 山口, 利男

× 山口, 利男

山口, 利男

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フリガナ ヤマグチ, トシオ

× ヤマグチ, トシオ

ヤマグチ, トシオ

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著者 YAMAGUCHI, Toshio

× YAMAGUCHI, Toshio

en YAMAGUCHI, Toshio

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学位授与機関
学位授与機関名 総合研究大学院大学
学位名
学位名 博士(理学)
学位記番号
内容記述タイプ Other
内容記述 総研大甲第539号
研究科
値 生命科学研究科
専攻
値 X2 分子生物機構論専攻
学位授与年月日
学位授与年月日 2001-03-23
学位授与年度
値 2000
要旨
内容記述タイプ Other
内容記述 The coloration of blue flowers depends on the production of the appropriate anthocyanin pigments, the presence of metal ions and co-pigments, and vacuolar pH. An increase in vacuolar pH enhances blue coloration, however, little is known about the proteins that are responsible for raising the vacuolar pH. The Japanese morning glory (Ipomoea nil) normally displays bright blue flower. One of the mutable allele, named purple-mutable (pr-m) confers purple flowers with blue sectors owing to recurrent somatic mutation from recessive pr-m allele to the blue Pr-r (Purple- revertant) allele. In this study, I described detail of the identification and characterization of the Pr gene, that is necessary for displaying blue flower in the I. nil, and discussed the observations with regard to the blue flower coloration. Germinal revertant carrying Pr-r allele homozygously that displays blue flower was successfully obtained from pr-m line, and compared with pr-m line. No alterations were found in the anthocyanin pigment compositions between the pr-m and Pr-r lines whereas the vacuolar pH in the pr-m was significantly lower than that in Pr-r. These results indicate that the function of the Pr gene is to raise vacuolar pH. By comparing the pr-m and Pr- r with simplified transposon display methods (STDM), the Pr gene also cDNA were successfully isolated. A Tpnl-related transposon, Tpn4 insertion was found within the exon 1 of the Pr gene in pr-m plant. This gene contained an open reading frame that is similar to the vacuolar Na+/H+ exchanger found in yeast, Arabidopsis, and rice. The Pr gene cDNA was able to complement the nhxl mutation for vacuolar Na+/H+ exchanger in yeast (Saccharomyces cerevisiae). A second copy for Pr gene was found in I. nil genome, but this copy was found to be pseudogene. The Pr gene was the most abundantly expressed in flower petals, and extensively found at around 12 h before flower opening, whereas no such increased expression was observed in the flower of Petunia hybrida. This result indicates the abundant expression of NHX1 genes before flower blooming was not common in all flowering plants. Since the NHX1 genes that promote the transport of Na+ into the vacuoles has been known to be involved in salt tolerance, we can add another important role for blue flower coloration in the Japanese morning glory by the vacuolar alkalization.
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内容記述タイプ Other
内容記述 application/pdf
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