| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2012-12-18 |
| タイトル |
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タイトル |
The Power of QTL Mapping with RILs |
| タイトル |
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タイトル |
The Power of QTL Mapping with RILs |
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言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
TAKUNO, Shohei
TERAUCHI, Ryohei
INNAN, Hideki
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| 著者別名 |
印南, 秀樹
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
QTL (quantitative trait loci) mapping is commonly used to identify genetic regions responsible to important phenotype variation. A common strategy of QTL mapping is to use recombinant inbred lines (RILs), which are usually established by several generations of inbreeding of an F1 population (usually up to F6 or F7 populations). As this inbreeding process involves a large amount of labor, we are particularly interested in the effect of the number of inbreeding generations on the power of QTL mapping; a part of the labor could be saved if a smaller number of inbreeding provides sufficient power. By using simulations, we investigated the performance of QTL mapping with recombinant inbred lines (RILs). As expected, we found that the power of F4 population could be almost comparable to that of F6 and F7 populations. A potential problem in using F4 population is that a large proportion of RILs are heterozygotes. We here introduced a new method to partly relax this problem. The performance of this method was verified by simulations with a wide range of parameters including the size of the segregation population, recombination rate, genome size and the density of markers. We found our method works better than the commonly used standard method especially when there are a number of heterozygous markers. Our results imply that in most cases, QTL mapping does not necessarily require RILs at F6 or F7 generations; rather, F4 (or even F3) populations would be almost as useful as F6 or F7 populations. Because the cost to establish a number of RILs for many generations is enormous, this finding will cause a reduction in the cost of QTL mapping, thereby accelerating gene mapping in many species. |
| 書誌情報 |
PLoS ONE
en : PLoS ONE
巻 7,
号 10,
発行日 2012-10-09
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| 出版者 |
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出版者 |
Public Library of Science |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
19326203 |
| DOI |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1371/journal.pone.0046545 |
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関連名称 |
10.1371/journal.pone.0046545 |
| 権利 |
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権利情報 |
© Takuno et al. |
| 関連サイト |
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識別子タイプ |
URI |
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関連識別子 |
http://www.plos.org/about/open-access/license/ |
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関連名称 |
Copyright |
| フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
| 著者版フラグ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
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journal.pone.0046545[1] (654.4 kB)
