{"_buckets": {"deposit": "0e09b453-7c80-48f8-b315-89cba2bedab7"}, "_deposit": {"created_by": 21, "id": "3488", "owners": [21], "pid": {"revision_id": 0, "type": "depid", "value": "3488"}, "status": "published"}, "_oai": {"id": "oai:ir.soken.ac.jp:00003488", "sets": ["34"]}, "author_link": ["368", "1275"], "item_2_creator_3": {"attribute_name": "著者名(英)", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "TANABE, Hideyuki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "368", "nameIdentifierScheme": "WEKO"}]}]}, "item_2_date_4": {"attribute_name": "発行日", "attribute_value_mlt": [{"subitem_date_issued_datetime": "2005", "subitem_date_issued_type": "Created"}]}, "item_2_description_13": {"attribute_name": "研究課題番号", "attribute_value_mlt": [{"subitem_description": "16510143", "subitem_description_type": "Other"}]}, "item_2_description_14": {"attribute_name": "フォーマット", "attribute_value_mlt": [{"subitem_description": "application/pdf", "subitem_description_type": "Other"}]}, "item_2_description_16": {"attribute_name": "研究期間", "attribute_value_mlt": [{"subitem_description": "2004年度～2005年度", "subitem_description_type": "Other"}]}, "item_2_description_17": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "本研究は、(1)霊長類細胞の収集、細胞培養、(2)染色体標本の調整、(3)2D-FISH法によるメタフェイズ解析、(4)3D-FISH法による染色体3次元核内配置解析、(5)比較解析と全体の統括という流れで進められた。霊長類各種末梢血を京都大学霊長類研究所の共同利用研究により供与していただいた;類人猿2種(チンパンジー、アジルテナガザル)、旧世界ザル6種(ニホンザル、カニクイザル、アカゲザル、タイワンザル、ミドリザル、マントヒヒ)、新世界ザル5種(コモンリスザル、ワタボウシタマリン、フサオマキザル、ケナガクモザル、ヨザル)。これらの霊長類リンパ球細胞核標本に対し、1)放射状核内配置の進化的保存性、2)相対核内配置と転座染色体生成との関係について考察した。1)では、ヒト18番および19番染色体ペイントプローブ、ヒトPeriphery vs InteriorミックスDNAプローブを使用した。その結果、類人猿、旧世界ザル、新世界ザルに至るまで、18番ホモログは核周辺部に、19番ホモログは核中心付近に配置されることが確認できた。また、ヒトP vs IミックスDNAプローブを用いた3D-FISH解析により、P、I両領域のトポロジーは、進化的染色体転座が高頻度に生じているテナガザルにおいても、高度な保存性を持つことが確認できた。2)については、ヒト2p、2qホモログDNAプローブを作成し、チンパンジーと旧世界ザル6種の細胞核に対して核内配置解析を実施した。その結果、ヒト2p、2qホモログの相対核内配置は、旧世界ザル各種では空間的に離れた距離を保っていたが、チンパンジーでは1組の2p、2qホモログが高頻度に隣接して配置されることが示唆された。このことにより、進化的な染色体再編成が生じている近縁種間での染色体ホモログ領域は、互いに相対核内配置が近接している傾向を示す可能性を持つものと考えられた。\r\n\r\nChromosome territories (CTs) in mammalian cell nuclei are highly compartmentalized as distinct domains and visualized by three-dimensional fluorescence in situ hybridization (3D-FISH) technique. The radial distribution of CTs is highly correlated with their gene densities and physical sizes. As an example, human 18 and 19 CTs show discrete difference in their positioning in lymphocyte nuclei; the gene-poor human 18 CTs are preferentially located at the nuclear periphery, while the gene-dense human 19 CTs are found in the nuclear interior. Here we investigated by 3D-FISH technique, 1) how radial distribution of CTs is evolutionarily conserved in primates, 2) how degree of relative positioning of CTs is involved in the evolutionary chromosomal translocations? First, we performed 3D-FISH analyses using the human 18 and 19 painting probes on the primate lymphocytes derived from various species, from chimpanzees, gibbons, Old World monkeys (6 species), and New World monkeys (5 species). The results showed that the topology of human 18 and 19 homologous CTs was highly conserved among those primate species. To study within the whole nuclear space, we developed the pooled chromosome paints specific for detecting the peripheral (P) or interior (I) nuclear space. The results by these probes also showed highly evolutionarily conserved even within the gibbon nuclei with highly evolutionary chromosomal rearrangements. Second, we used human chromosome 2p and 2q paints which is considered to be formed after fusing two separate acrocentric chromosomes, present in the great apes and Old World monkeys. Relative positioning of human 2p and 2q homologous CTs in primates showed the frequency with one pair chromosome kissing between 2p and 2q homologs is lower among the Old World monkeys but is higher among the great apes, suggesting that it has tendency with higher frequency of the chromosome kissing between 2p and 2q homologs among the evolutionary closed species from human.", "subitem_description_type": "Abstract"}]}, "item_2_description_18": {"attribute_name": "科研費研究者番号", "attribute_value_mlt": [{"subitem_description": "50261178 | http://kaken.nii.ac.jp/d/r/50261178.ja.html", "subitem_description_type": "Other"}]}, "item_2_description_6": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_description": "科学研究費補助金研究成果報告書 \r\n研究代表者：田辺秀之 ［総合研究大学院大学先導科学研究科准教授］ ", "subitem_description_type": "Other"}]}, "item_2_relation_11": {"attribute_name": "関連サイト", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "KAKEN（科学研究費補助金データーベース） "}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "http://kaken.nii.ac.jp/d/p/16510143.ja.html", "subitem_relation_type_select": "URI"}}]}, "item_2_text_19": {"attribute_name": "研究分野", "attribute_value_mlt": [{"subitem_text_value": "基礎ゲノム科学"}]}, "item_2_text_20": {"attribute_name": "研究種目", "attribute_value_mlt": [{"subitem_text_value": "基盤研究(C)"}]}, "item_2_text_21": {"attribute_name": "科研費の分科・細目", "attribute_value_mlt": [{"subitem_text_value": "基礎ゲノム科学"}]}, "item_2_text_5": {"attribute_name": "報告年度", "attribute_value_mlt": [{"subitem_text_value": "2005"}]}, "item_2_version_type_12": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "田辺 , 秀之 "}], "nameIdentifiers": [{"nameIdentifier": "1275", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2013-04-15"}], "displaytype": "simple", "download_preview_message": "", "file_order": 0, "filename": "C-19_kiban_C_tanabe.pdf", "filesize": [{"value": "185.3 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 185300.0, "url": {"label": "研究成果報告書", "url": "https://ir.soken.ac.jp/record/3488/files/C-19_kiban_C_tanabe.pdf"}, "version_id": "6aeb0791-d535-411b-8d91-24f5ae1e66f7"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "research report", "resourceuri": "http://purl.org/coar/resource_type/c_18ws"}]}, "item_title": "霊長類を中心とした哺乳類における染色体3次元核内配置からみたゲノム構造と進化機構", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "霊長類を中心とした哺乳類における染色体3次元核内配置からみたゲノム構造と進化機構"}, {"subitem_title": "Genomic architecture and evolutionary organization by three-dimensional nuclear positioning of chromosome territories in mammalian species especially in primates", "subitem_title_language": "en"}]}, "item_type_id": "2", "owner": "21", "path": ["34"], "permalink_uri": "https://ir.soken.ac.jp/records/3488", "pubdate": {"attribute_name": "公開日", "attribute_value": "2013-04-15"}, "publish_date": "2013-04-15", "publish_status": "0", "recid": "3488", "relation": {}, "relation_version_is_last": true, "title": ["霊長類を中心とした哺乳類における染色体3次元核内配置からみたゲノム構造と進化機構"], "weko_shared_id": -1}