| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2013-08-12 |
| タイトル |
|
|
タイトル |
Biochemical and functional characterization of BLUF-type flavin-binding proteins of two species of cyanobacteria |
| タイトル |
|
|
タイトル |
Biochemical and functional characterization of BLUF-type flavin-binding proteins of two species of cyanobacteria |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
|
資源タイプ |
journal article |
| アクセス権 |
|
|
アクセス権 |
metadata only access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_14cb |
| 著者 |
OKAJIMA, Koji
YOSHIHARA, Shizue
FUKUSHIMA, Yoshimasa
GENG, Xiaoxing
KATAYAMA, Mitsunori
HIGASHI, Shoichi
WATANABE, Masakatsu
SATO, Shusei
TABATA, Satoshi
SHIBATA, Yutaka
ITOH, Shigeru
IKEUCHI, Masahiko
|
| 著者別名 |
渡辺, 正勝
|
| 抄録 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
BLUF (a sensor of Blue-Light Using FAD) is a novel putative photoreceptor domain that is found in many bacteria and some eukaryotic algae. As found on genome analysis, certain cyanobacteria have BLUF proteins with a short C-terminal extension. As typical examples, TII0078 from thermophilic Thermosynechococcus elongatus BP-1 and Slr1694 from mesophilic Synechocystis sp. PCC 6803 were comparatively studied. FAD of both proteins was hardly reduced by exogenous reductants or mediators except methylviologen but showed a typical spectral shift to a longer wavelength upon excitation with blue light. In particular, freshly prepared TII0078 protein showed slow but reversible aggregation, indicative of light-induced conformational changes in the protein structure. TII0078 is far more stable as to heat treatment than Slr1694, as judged from flavin fluorescence. The slr1694-disruptant showed phototactic motility away from the light source (negative phototaxis), while the wild type Synechocystis showed positive phototaxis toward the source. Yeast two-hybrid screening with slr1694 showed self-interaction of Slr1694 (PixD) with itself and interaction with a novel PatA-like response regulator, Slr1693 (PixE). These results were discussed in relation to the signaling mechanism of the "short" BLUF proteins in the regulation of cyanobacterial phototaxis. |
| 書誌情報 |
Journal of Biochemistry
en : Journal of Biochemistry
巻 137,
号 6,
p. 741-750,
発行日 2005-06
|
| 出版者 |
|
|
出版者 |
Oxford University Press |
| ISSN |
|
|
収録物識別子タイプ |
ISSN |
|
収録物識別子 |
0021924X |
| PubMed番号 |
|
|
|
識別子タイプ |
PMID |
|
|
関連識別子 |
16002996 |
| DOI |
|
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1093/jb/mvi089 |
|
|
関連名称 |
10.1093/jb/mvi089 |
| 権利 |
|
|
権利情報 |
© 2005 The Japanese Biochemical Society |
