{"_buckets": {"deposit": "7d42f7b4-0dda-4d78-84ed-9f49c9343049"}, "_deposit": {"created_by": 21, "id": "3872", "owners": [21], "pid": {"revision_id": 0, "type": "depid", "value": "3872"}, "status": "published"}, "_oai": {"id": "oai:ir.soken.ac.jp:00003872", "sets": ["283"]}, "author_link": ["1871", "1870", "345", "1867", "1873", "1868", "1830", "1869", "1872", "1843", "1875", "346", "1874"], "item_10001_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2005-06", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "6", "bibliographicPageEnd": "750", "bibliographicPageStart": "741", "bibliographicVolumeNumber": "137", "bibliographic_titles": [{"bibliographic_title": "Journal of Biochemistry "}, {"bibliographic_title": "Journal of Biochemistry ", "bibliographic_titleLang": "en"}]}]}, "item_10001_creator_3": {"attribute_name": "著者別名", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "渡辺, 正勝"}], "nameIdentifiers": [{"nameIdentifier": "345", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "1000040124226", "nameIdentifierScheme": "NRID", "nameIdentifierURI": " "}, {"nameIdentifier": "40124226", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=40124226"}]}]}, "item_10001_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "BLUF (a sensor of Blue-Light Using FAD) is a novel putative photoreceptor domain that is found in many bacteria and some eukaryotic algae. As found on genome analysis, certain cyanobacteria have BLUF proteins with a short C-terminal extension. As typical examples, TII0078 from thermophilic Thermosynechococcus elongatus BP-1 and Slr1694 from mesophilic Synechocystis sp. PCC 6803 were comparatively studied. FAD of both proteins was hardly reduced by exogenous reductants or mediators except methylviologen but showed a typical spectral shift to a longer wavelength upon excitation with blue light. In particular, freshly prepared TII0078 protein showed slow but reversible aggregation, indicative of light-induced conformational changes in the protein structure. TII0078 is far more stable as to heat treatment than Slr1694, as judged from flavin fluorescence. The slr1694-disruptant showed phototactic motility away from the light source (negative phototaxis), while the wild type Synechocystis showed positive phototaxis toward the source. Yeast two-hybrid screening with slr1694 showed self-interaction of Slr1694 (PixD) with itself and interaction with a novel PatA-like response regulator, Slr1693 (PixE). These results were discussed in relation to the signaling mechanism of the \"short\" BLUF proteins in the regulation of cyanobacterial phototaxis.", "subitem_description_type": "Abstract"}]}, "item_10001_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Oxford University Press"}]}, "item_10001_relation_13": {"attribute_name": "PubMed番号", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "16002996", "subitem_relation_type_select": "PMID"}}]}, "item_10001_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1093/jb/mvi089"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1093/jb/mvi089", "subitem_relation_type_select": "DOI"}}]}, "item_10001_rights_15": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "© 2005 The Japanese Biochemical Society"}]}, "item_10001_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0021924X ", "subitem_source_identifier_type": "ISSN"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "OKAJIMA, Koji "}], "nameIdentifiers": [{"nameIdentifier": "1867", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "YOSHIHARA, Shizue "}], "nameIdentifiers": [{"nameIdentifier": "1868", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "FUKUSHIMA, Yoshimasa "}], "nameIdentifiers": [{"nameIdentifier": "1869", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "GENG, Xiaoxing "}], "nameIdentifiers": [{"nameIdentifier": "1870", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "KATAYAMA, Mitsunori "}], "nameIdentifiers": [{"nameIdentifier": "1871", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "HIGASHI, Shoichi "}], "nameIdentifiers": [{"nameIdentifier": "1843", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "WATANABE, Masakatsu"}], "nameIdentifiers": [{"nameIdentifier": "346", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "SATO, Shusei "}], "nameIdentifiers": [{"nameIdentifier": "1872", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "TABATA, Satoshi "}], "nameIdentifiers": [{"nameIdentifier": "1830", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "SHIBATA, Yutaka "}], "nameIdentifiers": [{"nameIdentifier": "1873", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "ITOH, Shigeru"}], "nameIdentifiers": [{"nameIdentifier": "1874", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "IKEUCHI, Masahiko "}], "nameIdentifiers": [{"nameIdentifier": "1875", "nameIdentifierScheme": "WEKO"}]}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Biochemical and functional characterization of BLUF-type flavin-binding proteins of two species of cyanobacteria", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Biochemical and functional characterization of BLUF-type flavin-binding proteins of two species of cyanobacteria"}, {"subitem_title": "Biochemical and functional characterization of BLUF-type flavin-binding proteins of two species of cyanobacteria", "subitem_title_language": "en"}]}, "item_type_id": "10001", "owner": "21", "path": ["109", "283"], "permalink_uri": "https://ir.soken.ac.jp/records/3872", "pubdate": {"attribute_name": "公開日", "attribute_value": "2013-08-12"}, "publish_date": "2013-08-12", "publish_status": "0", "recid": "3872", "relation": {}, "relation_version_is_last": true, "title": ["Biochemical and functional characterization of BLUF-type flavin-binding proteins of two species of cyanobacteria"], "weko_shared_id": 21}