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Differentiation of photocycle characteristics of flavin-binding BLUF domains of α-And β-subunits of photoactivated adenylyl cyclase of Euglena gracilis
https://ir.soken.ac.jp/records/3883
https://ir.soken.ac.jp/records/3883b4058fa3-a267-465a-b917-2c5df641363f
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2013-08-13 | |||||
| タイトル | ||||||
| タイトル | Differentiation of photocycle characteristics of flavin-binding BLUF domains of α-And β-subunits of photoactivated adenylyl cyclase of Euglena gracilis | |||||
| タイトル | ||||||
| タイトル | Differentiation of photocycle characteristics of flavin-binding BLUF domains of α-And β-subunits of photoactivated adenylyl cyclase of Euglena gracilis | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
ITO, Shinji
× ITO, Shinji× MURAKAMI, Akio× ISEKI, Mineo× TAKAHASHI, Tetsuo× HIGASHI, Shoichi× WATANABE, Masakatsu |
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| 著者別名 |
渡辺, 正勝
× 渡辺, 正勝 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Photoactivated adenylyl cyclase (PAC), an FAD-containing photoreceptor of Euglena gracilis, appears to be a heterotetrameric structure composed of 2 homologous subunits (PACα and PACβ), each with a pair of BLUF domains (F1 and F2). PAC promotes blue light-induced activation of adenylyl cyclase. In our previous report, we demonstrated that a recombinant version of the PACαF2 domain displays blue light-induced photocycle similar to those of prokaryotic BLUFs (Ito et al., Photochem. Photobiol. Sci., 2005, 4, 762-769). Here, we further examine the recombinant PACβF2 domain, which like PACαF2 exhibits a blue light-induced photocycle. The estimated quantum efficiency for the phototransformation of PACβF2 was 0.06-0.08, and the half-life for dark relaxation was 3-6 s while the corresponding values for the PACαF2 were 0.28-0.32 and 34-44 s. The remarkable differences between PACαF2 and PACβF2 may be related to the sensitivity of the photoactivation. In PACαF2, amino acid position 556, which is equivalent to Trp104 in the BLUF domain of the purple bacterial AppA protein, is occupied by a Leu residue, while in PACβF2 the equivalent BLUF domain site is conserved as Trp560. Amino acid substitution at this site in PACβF2-Trp560Leu markedly increased the estimated quantum efficiency (0.23) and accelerated the half-life of the dark-relaxation (2 s). These results indicate that Trp560 in PACβF2 plays a main role in suppressing the quantum efficiency. | |||||
| 書誌情報 |
Photochemical and Photobiological Sciences en : Photochemical and Photobiological Sciences 巻 9, 号 10, p. 1327-1335, 発行日 2010-10 |
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| 出版者 | ||||||
| 出版者 | Royal Society of Chemistry | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1474905X | |||||
| PubMed番号 | ||||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 20842310 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | http://doi.org/10.1039/c0pp00130a | |||||
| 関連名称 | 10.1039/c0pp00130a | |||||
| 権利 | ||||||
| 権利情報 | © 2010 The Royal Society of Chemistry and Owner Societies | |||||
