| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2013-08-26 |
| タイトル |
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タイトル |
Photoactivated Adenylyl Cyclase Controls Phototaxis in the Flagellate Euglena gracilis |
| タイトル |
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タイトル |
Photoactivated Adenylyl Cyclase Controls Phototaxis in the Flagellate Euglena gracilis |
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言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
NTEFIDOU, Maria
ISEKI, Mineo
WATANABE, Masakatsu
LEBERT, Michael
HäDER, Donat-Peter
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| 著者別名 |
渡辺, 正勝
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Euglena gracilis, a unicellular freshwater protist exhibits different photomovement responses, such as phototaxis (oriented movement toward or away from the light source) and photophobic (abrupt turn in response to a rapid increase [step-up] or decrease [step-down] in the light fluence rate) responses. Photoactivated adenylyl cyclase (PAC) has been isolated from whole-cell preparations and identified by RNA interference (RNA) to be the photoreceptor for step-up photophobic responses but not for step-down photophobic responses (M. Iseki, S, Matsunaga, A. Murakami, K. Ohno, K. Shiga, C. Yoshida, M. Sugai, T. Takahashi, T. Hori, M. Watanabe [2002] Nature 415: 1047-1051). The present study shows that knockdown of PAC by RNAi also effectively suppresses both positive and negative phototaxis, indicating for the first time that PAC or a PAC homolog is also the photoreceptor for photoorientation of wild-type E. gracilis. Recovery from RNAi occurred earlier for step-up photophobic responses than for positive and negative phototaxis. In addition, we investigated several phototaxis mutant strains of E. gracilis with different cytological features regarding the stigma and paraxonemal body (PAB; believed to be the location for the phototaxis photoreceptor) as well as Astasia longa, a close relative of E. gracilis. All of the E. gracilis mutant strains had PAC mRNAs, whereas in A. longa, a different but similar mRNA was found and designated AlPAC. Consistently, all of these strains showed no photo taxis but performed step-up photophobic responses, which were suppressed by RNAi of the PAC mRNA. The fact that some of these strains possess a cytologically altered or no PAB demonstrates that at least in these strains, the PAC photoreceptor responsible for the step-up photophobic responses is not located in the PAB. |
| 書誌情報 |
Plant Physiology
en : Plant Physiology
巻 133,
号 4,
p. 1517-1521,
発行日 2003-12
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| 出版者 |
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出版者 |
American Society of Plant Biologists |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
00320889 |
| PubMed番号 |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
PMID |
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関連識別子 |
14630964 |
| DOI |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1104/pp.103.034223 |
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関連名称 |
10.1104/pp.103.034223 |
| 権利 |
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権利情報 |
© 2013 by the American Society of Plant Biologists |
| 関連サイト |
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識別子タイプ |
URI |
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関連識別子 |
http://www.plantcell.org/site/misc/ifora.xhtml |
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関連名称 |
Copyright |
| フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
| 著者版フラグ |
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出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
1517.full (163.3 kB)
