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Photoactivated Adenylyl Cyclase Controls Phototaxis in the Flagellate Euglena gracilis
https://ir.soken.ac.jp/records/3925
https://ir.soken.ac.jp/records/39252a1f653f-3d9c-4803-94a9-711e47615f15
名前 / ファイル | ライセンス | アクション |
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2013-08-26 | |||||
タイトル | ||||||
タイトル | Photoactivated Adenylyl Cyclase Controls Phototaxis in the Flagellate Euglena gracilis | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Photoactivated Adenylyl Cyclase Controls Phototaxis in the Flagellate Euglena gracilis | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
NTEFIDOU, Maria
× NTEFIDOU, Maria× ISEKI, Mineo× WATANABE, Masakatsu× LEBERT, Michael× HäDER, Donat-Peter |
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著者別名 |
渡辺, 正勝
× 渡辺, 正勝 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Euglena gracilis, a unicellular freshwater protist exhibits different photomovement responses, such as phototaxis (oriented movement toward or away from the light source) and photophobic (abrupt turn in response to a rapid increase [step-up] or decrease [step-down] in the light fluence rate) responses. Photoactivated adenylyl cyclase (PAC) has been isolated from whole-cell preparations and identified by RNA interference (RNA) to be the photoreceptor for step-up photophobic responses but not for step-down photophobic responses (M. Iseki, S, Matsunaga, A. Murakami, K. Ohno, K. Shiga, C. Yoshida, M. Sugai, T. Takahashi, T. Hori, M. Watanabe [2002] Nature 415: 1047-1051). The present study shows that knockdown of PAC by RNAi also effectively suppresses both positive and negative phototaxis, indicating for the first time that PAC or a PAC homolog is also the photoreceptor for photoorientation of wild-type E. gracilis. Recovery from RNAi occurred earlier for step-up photophobic responses than for positive and negative phototaxis. In addition, we investigated several phototaxis mutant strains of E. gracilis with different cytological features regarding the stigma and paraxonemal body (PAB; believed to be the location for the phototaxis photoreceptor) as well as Astasia longa, a close relative of E. gracilis. All of the E. gracilis mutant strains had PAC mRNAs, whereas in A. longa, a different but similar mRNA was found and designated AlPAC. Consistently, all of these strains showed no photo taxis but performed step-up photophobic responses, which were suppressed by RNAi of the PAC mRNA. The fact that some of these strains possess a cytologically altered or no PAB demonstrates that at least in these strains, the PAC photoreceptor responsible for the step-up photophobic responses is not located in the PAB. | |||||
書誌情報 |
Plant Physiology en : Plant Physiology 巻 133, 号 4, p. 1517-1521, 発行日 2003-12 |
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出版者 | ||||||
出版者 | American Society of Plant Biologists | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00320889 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | 14630964 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1104/pp.103.034223 | |||||
関連名称 | 10.1104/pp.103.034223 | |||||
権利 | ||||||
権利情報 | © 2013 by the American Society of Plant Biologists | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | http://www.plantcell.org/site/misc/ifora.xhtml | |||||
関連名称 | Copyright | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |