{"_buckets": {"deposit": "f608d2d0-3382-4f97-8c45-cc092b623c88"}, "_deposit": {"created_by": 21, "id": "3925", "owners": [21], "pid": {"revision_id": 0, "type": "depid", "value": "3925"}, "status": "published"}, "_oai": {"id": "oai:ir.soken.ac.jp:00003925", "sets": ["283"]}, "author_link": ["2007", "345", "824", "2008", "346", "2009"], "item_10001_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2003-12", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "4", "bibliographicPageEnd": "1521", "bibliographicPageStart": "1517", "bibliographicVolumeNumber": "133", "bibliographic_titles": [{"bibliographic_title": "Plant Physiology "}, {"bibliographic_title": "Plant Physiology ", "bibliographic_titleLang": "en"}]}]}, "item_10001_creator_3": {"attribute_name": "著者別名", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "渡辺, 正勝"}], "nameIdentifiers": [{"nameIdentifier": "345", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "1000040124226", "nameIdentifierScheme": "NRID", "nameIdentifierURI": " "}, {"nameIdentifier": "40124226", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=40124226"}]}]}, "item_10001_description_19": {"attribute_name": "フォーマット", "attribute_value_mlt": [{"subitem_description": "application/pdf", "subitem_description_type": "Other"}]}, "item_10001_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Euglena gracilis, a unicellular freshwater protist exhibits different photomovement responses, such as phototaxis (oriented movement toward or away from the light source) and photophobic (abrupt turn in response to a rapid increase [step-up] or decrease [step-down] in the light fluence rate) responses. Photoactivated adenylyl cyclase (PAC) has been isolated from whole-cell preparations and identified by RNA interference (RNA) to be the photoreceptor for step-up photophobic responses but not for step-down photophobic responses (M. Iseki, S, Matsunaga, A. Murakami, K. Ohno, K. Shiga, C. Yoshida, M. Sugai, T. Takahashi, T. Hori, M. Watanabe [2002] Nature 415: 1047-1051). The present study shows that knockdown of PAC by RNAi also effectively suppresses both positive and negative phototaxis, indicating for the first time that PAC or a PAC homolog is also the photoreceptor for photoorientation of wild-type E. gracilis. Recovery from RNAi occurred earlier for step-up photophobic responses than for positive and negative phototaxis. In addition, we investigated several phototaxis mutant strains of E. gracilis with different cytological features regarding the stigma and paraxonemal body (PAB; believed to be the location for the phototaxis photoreceptor) as well as Astasia longa, a close relative of E. gracilis. All of the E. gracilis mutant strains had PAC mRNAs, whereas in A. longa, a different but similar mRNA was found and designated AlPAC. Consistently, all of these strains showed no photo taxis but performed step-up photophobic responses, which were suppressed by RNAi of the PAC mRNA. The fact that some of these strains possess a cytologically altered or no PAB demonstrates that at least in these strains, the PAC photoreceptor responsible for the step-up photophobic responses is not located in the PAB.", "subitem_description_type": "Abstract"}]}, "item_10001_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "American Society of Plant Biologists"}]}, "item_10001_relation_13": {"attribute_name": "PubMed番号", "attribute_value_mlt": [{"subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "14630964", "subitem_relation_type_select": "PMID"}}]}, "item_10001_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1104/pp.103.034223 "}], "subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1104/pp.103.034223", "subitem_relation_type_select": "DOI"}}]}, "item_10001_relation_17": {"attribute_name": "関連サイト", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "Copyright"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "http://www.plantcell.org/site/misc/ifora.xhtml", "subitem_relation_type_select": "URI"}}]}, "item_10001_rights_15": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "© 2013 by the American Society of Plant Biologists"}]}, "item_10001_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "00320889 ", "subitem_source_identifier_type": "ISSN"}]}, "item_10001_version_type_20": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "NTEFIDOU, Maria"}], "nameIdentifiers": [{"nameIdentifier": "2007", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "ISEKI, Mineo "}], "nameIdentifiers": [{"nameIdentifier": "824", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "WATANABE, Masakatsu"}], "nameIdentifiers": [{"nameIdentifier": "346", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "LEBERT, Michael "}], "nameIdentifiers": [{"nameIdentifier": "2008", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "HäDER, Donat-Peter "}], "nameIdentifiers": [{"nameIdentifier": "2009", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2013-08-26"}], "displaytype": "simple", "download_preview_message": "", "file_order": 0, "filename": "1517.full.pdf", "filesize": [{"value": "163.3 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 163300.0, "url": {"label": "1517.full", "url": "https://ir.soken.ac.jp/record/3925/files/1517.full.pdf"}, "version_id": "fb9e75b4-4c1a-479e-92b9-f413d558cb27"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Photoactivated Adenylyl Cyclase Controls Phototaxis in the Flagellate Euglena gracilis", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Photoactivated Adenylyl Cyclase Controls Phototaxis in the Flagellate Euglena gracilis"}, {"subitem_title": "Photoactivated Adenylyl Cyclase Controls Phototaxis in the Flagellate Euglena gracilis", "subitem_title_language": "en"}]}, "item_type_id": "10001", "owner": "21", "path": ["109", "283"], "permalink_uri": "https://ir.soken.ac.jp/records/3925", "pubdate": {"attribute_name": "公開日", "attribute_value": "2013-08-26"}, "publish_date": "2013-08-26", "publish_status": "0", "recid": "3925", "relation": {}, "relation_version_is_last": true, "title": ["Photoactivated Adenylyl Cyclase Controls Phototaxis in the Flagellate Euglena gracilis"], "weko_shared_id": 21}