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  1. 010 学術雑誌論文
  2. 田辺, 秀之 / TANABE, Hideyuki

Isolation of micronuclei from mouse blood and fluorescence in situ hybridization with a mouse centromeric DNA probe

https://ir.soken.ac.jp/records/3988
https://ir.soken.ac.jp/records/3988
2a2c43c1-173c-4de4-bac6-2298eb2930e8
Item type 学術雑誌論文 / Journal Article(1)
公開日 2013-09-25
タイトル
タイトル Isolation of micronuclei from mouse blood and fluorescence in situ hybridization with a mouse centromeric DNA probe
タイトル
言語 en
タイトル Isolation of micronuclei from mouse blood and fluorescence in situ hybridization with a mouse centromeric DNA probe
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ journal article
アクセス権
アクセス権 metadata only access
アクセス権URI http://purl.org/coar/access_right/c_14cb
著者 HAYASHI, Makoto

× HAYASHI, Makoto

WEKO 2108

HAYASHI, Makoto

Search repository
MäKI-PAAKKANEN, Jorma

× MäKI-PAAKKANEN, Jorma

WEKO 2144

MäKI-PAAKKANEN, Jorma

Search repository
TANABE, Hideyuki

× TANABE, Hideyuki

WEKO 368

TANABE, Hideyuki

Search repository
et, al.

× et, al.

WEKO 3119

et, al.

Search repository
著者別名 田辺, 秀之

× 田辺, 秀之

WEKO 367
NRID 1000050261178
e-Rad 50261178

田辺, 秀之

Search repository
抄録
内容記述タイプ Abstract
内容記述 Spontaneously existing and chemically induced micronuclei were isolated from mouse blood. 50 μl of cardiac blood was diluted with PBS and centrifuged. After this, the cell pellet was subjected to hypotonic treatment, fixed with acetic acid-methanol (1:3), and the lysate was filtrated through a 2-μm polycarbonate nuclepore membrane. Isolated micronuclei were air-dried on a glass slide and subjected to fluorescence in situ hybridization (FISH) using a mouse centromeric gamma satellite probe. Approximately half of the micronuclei isolated from vehicle control mice showed centromere signal(s). In these preliminary studies, the proportion of centromere-positive micronuclei was increased by treatment with spindle poisons (colchicine and vinblastine sulfate), decreased only slightly by 1-β-D-arabinofuranosylcytosine, and was generally unaffected by mitomycin C.
書誌情報 Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
en : Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis

巻 307, 号 1, p. 245-251, 発行日 1994-05-01
出版者
出版者 Elsevier
ISSN
収録物識別子タイプ ISSN
収録物識別子 0921-8262
DOI
識別子タイプ DOI
関連識別子 https://doi.org/10.1016/0027-5107(94)90298-4
関連名称 10.1016/0027-5107(94)90298-4
権利
権利情報 © 1994 Published by Elsevier B.V.
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