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Isolation of micronuclei from mouse blood and fluorescence in situ hybridization with a mouse centromeric DNA probe
https://ir.soken.ac.jp/records/3988
https://ir.soken.ac.jp/records/39882a2c43c1-173c-4de4-bac6-2298eb2930e8
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2013-09-25 | |||||
| タイトル | ||||||
| タイトル | Isolation of micronuclei from mouse blood and fluorescence in situ hybridization with a mouse centromeric DNA probe | |||||
| タイトル | ||||||
| タイトル | Isolation of micronuclei from mouse blood and fluorescence in situ hybridization with a mouse centromeric DNA probe | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
HAYASHI, Makoto
× HAYASHI, Makoto× MäKI-PAAKKANEN, Jorma× TANABE, Hideyuki× et, al. |
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| 著者別名 |
田辺, 秀之
× 田辺, 秀之 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Spontaneously existing and chemically induced micronuclei were isolated from mouse blood. 50 μl of cardiac blood was diluted with PBS and centrifuged. After this, the cell pellet was subjected to hypotonic treatment, fixed with acetic acid-methanol (1:3), and the lysate was filtrated through a 2-μm polycarbonate nuclepore membrane. Isolated micronuclei were air-dried on a glass slide and subjected to fluorescence in situ hybridization (FISH) using a mouse centromeric gamma satellite probe. Approximately half of the micronuclei isolated from vehicle control mice showed centromere signal(s). In these preliminary studies, the proportion of centromere-positive micronuclei was increased by treatment with spindle poisons (colchicine and vinblastine sulfate), decreased only slightly by 1-β-D-arabinofuranosylcytosine, and was generally unaffected by mitomycin C. | |||||
| 書誌情報 |
Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis en : Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis 巻 307, 号 1, p. 245-251, 発行日 1994-05-01 |
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| 出版者 | ||||||
| 出版者 | Elsevier | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0921-8262 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1016/0027-5107(94)90298-4 | |||||
| 関連名称 | 10.1016/0027-5107(94)90298-4 | |||||
| 権利 | ||||||
| 権利情報 | © 1994 Published by Elsevier B.V. | |||||
