| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2013-09-25 |
| タイトル |
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タイトル |
A novel assay system for anti-human immunodeficiency virus type 1 (HIV-1) activity using a subclone of a monocytic cell line, U937 |
| タイトル |
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タイトル |
A novel assay system for anti-human immunodeficiency virus type 1 (HIV-1) activity using a subclone of a monocytic cell line, U937 |
|
言語 |
en |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
metadata only access |
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アクセス権URI |
http://purl.org/coar/access_right/c_14cb |
| 著者 |
MIYANOKUROSAKI, N
NAKASHIMA , H
ICHIYAMA , K
INAZAWA, K
TANABE, Hideyuki
et, al.
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| 著者別名 |
田辺, 秀之
|
| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
A subclonal cl.1-14 cell was established from a monocytic cell line U937 by a limiting dilution method. The anti-HIV-1 activity of some antiviral compounds was evaluated in HIV-1-infected cl.1-14 cells. The results demonstrated that although AZT was a potent inhibitor of HIV-1 replication in cl.1-14 cells, its 50% effective concentration (EC50) values was 80 times higher than that in HIV-1 infected MT-4 cells; the EC50 of AZT was 0.16 microM and 0.002 microM in cl.1-14 and MT-4 cells, respectively. In contrast, the anti-HIV-1 activity of ddA, ddI and ddC in cl.1-14 cells was comparable to that in MT-4 cells. The antiviral activity of nevirapine, dextran sulfate, curdlan sulfate and T22 did not differ significantly between the cl.1-14 and MT-4 cells. The antiviral activity of several compounds in the HIV-1-infected cl.1-14 cells was similar to that in the HIV-1JR-FL-infected human peripheral macrophages. Our results suggest that cl.1-14 cell cultures are very useful for estimating antiviral activity and more advantageous than the use of peripheral blood macrophages. |
| 書誌情報 |
Microbiology and Immunology
en : Microbiology and Immunology
巻 38,
号 10,
p. 813-818,
発行日 1994
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| 出版者 |
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出版者 |
Wiley-Blackwell |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0385-5600 |
| PubMed番号 |
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識別子タイプ |
PMID |
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関連識別子 |
7869961 |
| 権利 |
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|
権利情報 |
© Wiley-Blackwell |
| 関連サイト |
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識別子タイプ |
URI |
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関連識別子 |
http://www.ncbi.nlm.nih.gov/pubmed/7869961 |
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関連名称 |
PMC |
