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The physiological basis for color vision in the Eastern Pale Clouded yellow butterfly, Colias erate
https://ir.soken.ac.jp/records/4099
https://ir.soken.ac.jp/records/4099fb8a9c10-5973-4a5d-96ed-48c42c92fd88
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要旨・審査要旨 (181.1 kB)
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本文 (16.8 MB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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| 公開日 | 2013-11-27 | |||||
| タイトル | ||||||
| タイトル | The physiological basis for color vision in the Eastern Pale Clouded yellow butterfly, Colias erate | |||||
| タイトル | ||||||
| タイトル | The physiological basis for color vision in the Eastern Pale Clouded yellow butterfly, Colias erate | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_46ec | |||||
| 資源タイプ | thesis | |||||
| 著者名 |
小川, 裕理
× 小川, 裕理 |
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| フリガナ |
オガワ, ユリ
× オガワ, ユリ |
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| 著者 |
OGAWA, Yuri
× OGAWA, Yuri |
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| 学位授与機関 | ||||||
| 学位授与機関名 | 総合研究大学院大学 | |||||
| 学位名 | ||||||
| 学位名 | 博士（理学） | |||||
| 学位記番号 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 総研大甲第1619号 | |||||
| 研究科 | ||||||
| 値 | 先導科学研究科 | |||||
| 専攻 | ||||||
| 値 | 23 生命共生体進化学専攻 | |||||
| 学位授与年月日 | ||||||
| 学位授与年月日 | 2013-03-22 | |||||
| 学位授与年度 | ||||||
| 値 | 2012 | |||||
| 要旨 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | The compound eye of Colias erate is composed of about 6,500 ommatidia, each containing nine photoreceptors (R1-9), forming a tiered rhabdom, surrounded by four reddish pigments. The ommatidia are divided into three types (I-III) due to the arrangement of perirhabdormal pigment spots and expression pattern of four opsin mRNAs. The opsins are already identified: they are short (CeUV), two middle (CeV1, CeV2) and L (CeL)-absorbing types. In the thesis research, I studied the physiological basis of color vision in the Eastern Pale Clouded yellow butterfly, Colias erate Esper, in detail focusing on the production mechanism of the variety of spectral photoreceptors. Following General Introduction, I describe a few basic properties of the Colias eye in Chapter 1. The basic properties are the eyeshine, which is a reflection from the tapetum at the bottom of the ommatidia, and the spectral sensitivity of the entire eye. The eyeshine of the dorsal region has identical reflectance spectrum in both sexes. In the ventral region of males, type I ommatidia reflect 660 nm and type II and III ommatidia reflect 730 nm. In females, type II ommatidia have broadband reflection from 620 to 730 nm, while type I and III respectively reflect 660 nm and 730 nm as in males. Remarkable sexual difference of eyeshine spectrum in type II ommatidia is attributable to the female-specific orange pigment. Regionalization was also found in the eye spectral sensitivity determined by electroretinography. Dorsal region of the retina is relatively more sensitive below 420 nm range, whereas the ventral region is more sensitive in the wavelength range of 420 nm to 550 nm. This is probably because of the differential distribution of different ommatidial types. Four of nine photoreceptor cells contribute their rhabdomeral microvilli to the distal tier of the tired Colias rhabdom. In chapter 2, I analyzed the visual pigments and spectral sensitivities of individual photoreceptors in the distal tier in both sexes of Colias erate. A subset of photoreceptor cells expresses a newly discovered middle wavelength-absorbing opsin, Colias erate Blue (CeB), in addition to two previously described middle wavelength-absorbing opsins, CeV1 and CeV2. The other photoreceptors either coexpress CeV1 and CeV2, or exclusively express a short wavelength-absorbing opsin, CeUV, or a long wavelength-absorbing opsin, CeL. Males and females have the same visual pigment expression patterns, but the photoreceptor spectral sensitivities are sexually dimorphic. The photoreceptors coexpressing three middle wavelength-absorbing opsins are broad-blue receptors in males, but in females they are narrow-blue receptors. Those with CeV1 and CeV2 are violet receptors in females, while they are shouldered-blue receptors in males. The sexual dimorphism in spectral sensitivity is caused by a sex-specific distribution of fluorescent pigment that functions as a spectral filter. In chapter 3, I demonstrated by intracellular recording that the spectral sensitivities of the proximal photoreceptors (R5-8) of all ommatidia in both sexes are strongly tuned by the perirhabdomal pigments. These act as long-pass filters, shifting the peak sensitivities into the wavelength range above 600 nm. Due to the sex-specific pigments in type II ommatidia, the spectral sensitivities of the R5-8 photoreceptors of females peaked at 620 nm while those in males peaked at 660 nm. The measured spectral sensitivities could be well reproduced by an optical model assuming a long wavelength-absorbing visual pigment with peak absorbance at 565 nm. Whereas the sexual dimorphism was unequivocally demonstrated for the ventral eye region, dimorphism in the dorsal region was not found. Presumably the ventral region is strongly related to sexual behaviors such as courtship and oviposition. I demonstrated in this thesis that males and females of Colias have different set of eight classes of spectral photoreceptor in the ventral region of the eye. On the other hand, I did not detect any sexual dimorphism in the dorsal retina. The eight types of photoreceptor identified in the Colias ventral retina are generated by 1) duplication of opsin genes, 2) expression of multiple opsins in single photoreceptors and 3) the filtering effect of the fluorescent and perirhabdomal pigment. Together with previous studies, especially on the small white, Pieris rapae, and the Japanese yellow swallowtail Papilio xuthus, these mechanisms underlying various spectral receptors appear to be widespread among butterflies. |
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| 値 | 有 | |||||
