WEKO3
アイテム
Equilibrium and kinetics of the unfolding of -lactalbumin by guanidine hydrochloride (IV): Dependence of the N equilibrium A transconformation on the temperature
https://ir.soken.ac.jp/records/4115
https://ir.soken.ac.jp/records/4115bd2eeb43-a656-47eb-b9b2-7e3f46a49364
| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2013-12-24 | |||||||||||||
| タイトル | ||||||||||||||
| タイトル | Equilibrium and kinetics of the unfolding of -lactalbumin by guanidine hydrochloride (IV): Dependence of the N equilibrium A transconformation on the temperature | |||||||||||||
| タイトル | ||||||||||||||
| タイトル | Equilibrium and kinetics of the unfolding of -lactalbumin by guanidine hydrochloride (IV): Dependence of the N equilibrium A transconformation on the temperature | |||||||||||||
| 言語 | en | |||||||||||||
| 言語 | ||||||||||||||
| 言語 | eng | |||||||||||||
| 資源タイプ | ||||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||
| 資源タイプ | journal article | |||||||||||||
| アクセス権 | ||||||||||||||
| アクセス権 | metadata only access | |||||||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||||||||||
| 著者 |
NITTA, Katsutoshi
× NITTA, Katsutoshi
× KITA, N
× KUWAJIMA, Kunihiro
× SUGAI, Shintaro
|
|||||||||||||
| 著者別名 |
桑島, 邦博
× 桑島, 邦博
|
|||||||||||||
| 抄録 | ||||||||||||||
| 内容記述タイプ | Abstract | |||||||||||||
| 内容記述 | The reversible unfolding from the native (N) state to the acid (A) state of α-lactalbumin by guanidine·HCl (0.8–2.0 M) was studied at 10–35 °C by means of difference spectra and pH-jump measurements. At each temperature, all points plotted as the logarithmic equilibrium constant log KNA of the N ⇌ A process against pH could fall on a curve independent of the denaturant concentration by shifting each point along the log KNA axis, where the shift factor ƒ did not depend on temperature. Moreover, by shifting the points at each temperature along the log () axis, a master curve, independent of both temperature and the denaturant concentration, could be obtained for the pH-dependence of log KNA. From the dependence of the logarithmic rate constants on pH, master curves independent of both temperature and the denaturant concentration could also be made for the and the processes, where A∗ means the activated state. The results show the two-state character of the N ⇌ A process. The enthalpy changes and the differences in heat capacity for the processes were determined from the accurate measurements of temperature dependence of the unfolding at pH 4.3 and 1.0 M guanidine·HCl. The results show that the disruption of hydrophobic interaction is caused mainly in the process, while most of the changes in the pK values of the ionizing groups are caused in the process. |
|||||||||||||
| 書誌情報 |
Biochimica et Biophysica Acta en : Biochimica et Biophysica Acta 巻 490, 号 1, p. 200-208, 発行日 1977-01-25 |
|||||||||||||
| 出版者 | ||||||||||||||
| 出版者 | Elsevier | |||||||||||||
| ISSN | ||||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||||
| 収録物識別子 | 0006-3002 | |||||||||||||
| DOI | ||||||||||||||
| 識別子タイプ | DOI | |||||||||||||
| 関連識別子 | https://doi.org/10.1016/0005-2795(77)90120-9 | |||||||||||||
| 関連名称 | 10.1016/0005-2795(77)90120-9 | |||||||||||||
| 権利 | ||||||||||||||
| 権利情報 | © 1977 Published by Elsevier B.V. | |||||||||||||
