{"_buckets": {"deposit": "440e0331-679e-4560-84a2-d6d90706a096"}, "_deposit": {"created_by": 21, "id": "4127", "owners": [21], "pid": {"revision_id": 0, "type": "depid", "value": "4127"}, "status": "published"}, "_oai": {"id": "oai:ir.soken.ac.jp:00004127", "sets": ["323"]}, "author_link": ["2361", "2364", "2376"], "item_10001_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1983-09-05", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "297", "bibliographicPageStart": "281", "bibliographicVolumeNumber": "169", "bibliographic_titles": [{"bibliographic_title": "Journal of Molecular Biology"}, {"bibliographic_title": "Journal of Molecular Biology", "bibliographic_titleLang": "en"}]}]}, "item_10001_creator_3": {"attribute_name": "著者別名", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "桑島, 邦博"}], "nameIdentifiers": [{"nameIdentifier": "2364", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "1000070091444", "nameIdentifierScheme": "NRID", "nameIdentifierURI": " "}, {"nameIdentifier": "70091444", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=70091444"}]}]}, "item_10001_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "The locations have been found of the eight most slowly exchanging peptide protons in residues 1 to 19 of ribonuclease S. The resonance lines of these eight protons are resolved by proton magnetic resonance at 360 MHz when either S-peptide (residues 1 to 19) or peptide 1-15 is bound to S-protein (residues 21 to 124). Other peptide protons have been removed by exchange in the sample preparation ([1H]S-peptide is added to deuterated S-protein in D2O), and also by exchange-out of the less protected protons in residues 1 to 19.\n\nAt pH 5·1, 0°C, there is a 100-fold difference in rates of exchange between the eight most protected protons and the less protected protons of S-peptide. The highly protected protons are protected 104-fold compared to free S-peptide. The protected protons have been identified by 1H nuclear magnetic resonance after denaturing ribonuclease S in ≥3 m-urea-d4, D2O, pH 2·3, −4°C, followed by comparing the chemical shifts of the remaining eight protons with the known -NH spectrum of the free peptide, which has been assigned from the two-dimensional homonuclear correlated spectrum and by comparison with earlier work.\n\nThe eight highly protected NH protons are localized in one segment, residues 7 to 14. All eight protons are H-bonded: those of residues 7 to 13 are H-bonded within the 3-13 α-helix and that of residue 14 is H-bonded to the β-sheet. The NH proton of residue 16, which also is H-bonded to the β-sheet, is not one of the highly protected protons. Both the N atoms of the eight NH groups and also the O atoms of their CO acceptor groups are shielded from solvent in most cases, according to the molecular area calculations of Finney (1978).", "subitem_description_type": "Abstract"}]}, "item_10001_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Elsevier"}]}, "item_10001_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1016/S0022-2836(83)80184-3"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1016/S0022-2836(83)80184-3", "subitem_relation_type_select": "DOI"}}]}, "item_10001_rights_15": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "© 1983 Published by Elsevier Ltd."}]}, "item_10001_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0022-2836", "subitem_source_identifier_type": "ISSN"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "KUWAJIMA, Kunihiro "}], "nameIdentifiers": [{"nameIdentifier": "2361", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "BALDWIN, Robert L"}], "nameIdentifiers": [{"nameIdentifier": "2376", "nameIdentifierScheme": "WEKO"}]}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Nature and locations of the most slowly exchanging peptide NH protons in residues 1 to 19 of ribonuclease S", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Nature and locations of the most slowly exchanging peptide NH protons in residues 1 to 19 of ribonuclease S"}, {"subitem_title": "Nature and locations of the most slowly exchanging peptide NH protons in residues 1 to 19 of ribonuclease S", "subitem_title_language": "en"}]}, "item_type_id": "10001", "owner": "21", "path": ["323"], "permalink_uri": "https://ir.soken.ac.jp/records/4127", "pubdate": {"attribute_name": "公開日", "attribute_value": "2013-12-25"}, "publish_date": "2013-12-25", "publish_status": "0", "recid": "4127", "relation": {}, "relation_version_is_last": true, "title": ["Nature and locations of the most slowly exchanging peptide NH protons in residues 1 to 19 of ribonuclease S"], "weko_shared_id": 21}