| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2014-01-15 |
| タイトル |
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タイトル |
An early immunoreactive folding intermediate of the tryptophan synthase β2 subunit is a ‘molten globule’ |
| タイトル |
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タイトル |
An early immunoreactive folding intermediate of the tryptophan synthase β2 subunit is a ‘molten globule’ |
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言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
GOLDBERG, Michel E
SEMISOTNOV, Gennady V
FRIGUET, Bertrand
KUWAJIMA, Kunihiro
PTITSYN, Oleg B
SUGAI, Shintaro
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| 著者別名 |
桑島, 邦博
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
The refolding kinetics of the tryptophan synthase β2 subunit have been investigated by circular dichroism (CD) and binding of a fluorescent hydrophobic probe (ANS), using the stopped-flow technique. The kinetics of regain of the native far UV CD signal show that, upon refolding of urea denatured β2, more than half of the protein secondary structure is formed within the dead time of the CD stopped-flow apparatus (0.013 s). On the other hand, upon refolding of guanidine unfolded β2 the fluorescence of ANS passes through a maximum after about 1 s and then ‘slowly’ decreases. These results show the accumulation, in the 1–10 s time range, of an early transient folding intermediate which has a pronounced secondary structure and a high affinity for ANS. In this time range, the near UV CD remains very low. This transient intermediate thus appears to have all the characteristics of the ‘molten globule’ state [(1987) FEBS Lett. 224, 9-13]. Moreover, by comparing the intrinsic time of the disappearance of this transient intermediate (t 35 s) with the time of formation of the previously characterized [(1988) Biochemistry 27, 7633-7640] early imuno-reactive intermediate recognized by a monoclonal antibody (t 12 s), it is shown that this native-like epitope forms within the ‘molten globule’, before the tight packing of the protein side chains. |
| 書誌情報 |
FEBS Letters
en : FEBS Letters
巻 263,
号 1,
p. 51-56,
発行日 1990-04-09
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| 出版者 |
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出版者 |
Elsevier |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0014-5793 |
| PubMed番号 |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
PMID |
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関連識別子 |
1691989 |
| DOI |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1016/0014-5793(90)80703-L |
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関連名称 |
10.1016/0014-5793(90)80703-L |
| 権利 |
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権利情報 |
© 1990 Published by Elsevier B.V. |
| 関連サイト |
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識別子タイプ |
URI |
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関連識別子 |
http://www.elsevier.com/journal-authors/author-rights-and-responsibilities |
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関連名称 |
Copyright |
| フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
| 著者版フラグ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
1-s2.0-001457939080703L-main (854.6 kB)
