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Calcium-regulated interactions of human α-lactalbumin with bee venom melittin
https://ir.soken.ac.jp/records/4146
https://ir.soken.ac.jp/records/41462d462099-684e-492c-a718-fa569d84b97d
Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2014-01-15 | |||||
タイトル | ||||||
タイトル | Calcium-regulated interactions of human α-lactalbumin with bee venom melittin | |||||
タイトル | ||||||
タイトル | Calcium-regulated interactions of human α-lactalbumin with bee venom melittin | |||||
言語 | en | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
アクセス権 | ||||||
アクセス権 | metadata only access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
著者 |
PERMYAKOV, Eugene A
× PERMYAKOV, Eugene A× GRISHCHENKO, Valery M× KALINICHENKO, Lina P× ORLOV, Nikolay Y× KUWAJIMA, Kunihiro× SUGAI, Shintaro |
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著者別名 |
桑島, 邦博
× 桑島, 邦博 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Affinity chromatography, fluorescence and circular dichroism spectroscopy methods have been used to study the interaction of melittin, a 26-residue peptide from bee venom, with Ca2+-binding α-lactalbumin from human milk. It has been revealed that melittin binds to the apo- and acidic states of α-lactalbumin while the presence of Ca2+ makes the interaction essentially weaker. The association constant for the complex of melittin with apo-α-lactalbumin determined from spectropolarimetric melittin-titration data is 2×107 M−1. The complexation of α-lactalbumin with melittin decreases its affinity to Ca2+ by three orders of magnitude. The interaction of apo-α-lactalbumin with melittin causes some changes in the environment of its aromatic amino acid residues and drastically alters the conformation of melittin, increasing its α-helical content but leaving its single tryptophan residue accessible to water. In the case of the acidic state of α-lactalbumin the interaction does not induce an increase in α-helical content of melittin. | |||||
書誌情報 |
Biophysical Chemistry en : Biophysical Chemistry 巻 39, 号 2, p. 111-117, 発行日 1991-02 |
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出版者 | ||||||
出版者 | Elsevier | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0301-4622 | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 2059662 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1016/0301-4622(91)85012-F | |||||
関連名称 | 10.1016/0301-4622(91)85012-F | |||||
権利 | ||||||
権利情報 | © 1991 Published by Elsevier B.V. |