{"_buckets": {"deposit": "82c33d52-cc2c-48a6-838d-6c6864d9c926"}, "_deposit": {"created_by": 21, "id": "4146", "owners": [21], "pid": {"revision_id": 0, "type": "depid", "value": "4146"}, "status": "published"}, "_oai": {"id": "oai:ir.soken.ac.jp:00004146", "sets": ["323"]}, "author_link": ["2361", "2393", "2364", "2397", "2363", "2398", "2399"], "item_10001_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1991-02", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "2", "bibliographicPageEnd": "117", "bibliographicPageStart": "111", "bibliographicVolumeNumber": "39", "bibliographic_titles": [{"bibliographic_title": "Biophysical Chemistry"}, {"bibliographic_title": "Biophysical Chemistry", "bibliographic_titleLang": "en"}]}]}, "item_10001_creator_3": {"attribute_name": "著者別名", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "桑島, 邦博"}], "nameIdentifiers": [{"nameIdentifier": "2364", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "1000070091444", "nameIdentifierScheme": "NRID", "nameIdentifierURI": " "}, {"nameIdentifier": "70091444", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=70091444"}]}]}, "item_10001_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Affinity chromatography, fluorescence and circular dichroism spectroscopy methods have been used to study the interaction of melittin, a 26-residue peptide from bee venom, with Ca2+-binding α-lactalbumin from human milk. It has been revealed that melittin binds to the apo- and acidic states of α-lactalbumin while the presence of Ca2+ makes the interaction essentially weaker. The association constant for the complex of melittin with apo-α-lactalbumin determined from spectropolarimetric melittin-titration data is 2×107 M−1. The complexation of α-lactalbumin with melittin decreases its affinity to Ca2+ by three orders of magnitude. The interaction of apo-α-lactalbumin with melittin causes some changes in the environment of its aromatic amino acid residues and drastically alters the conformation of melittin, increasing its α-helical content but leaving its single tryptophan residue accessible to water. In the case of the acidic state of α-lactalbumin the interaction does not induce an increase in α-helical content of melittin.", "subitem_description_type": "Abstract"}]}, "item_10001_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Elsevier "}]}, "item_10001_relation_13": {"attribute_name": "PubMed番号", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "2059662", "subitem_relation_type_select": "PMID"}}]}, "item_10001_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1016/0301-4622(91)85012-F"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1016/0301-4622(91)85012-F", "subitem_relation_type_select": "DOI"}}]}, "item_10001_rights_15": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "© 1991 Published by Elsevier B.V."}]}, "item_10001_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0301-4622", "subitem_source_identifier_type": "ISSN"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "PERMYAKOV, Eugene A"}], "nameIdentifiers": [{"nameIdentifier": "2393", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "GRISHCHENKO, Valery M"}], "nameIdentifiers": [{"nameIdentifier": "2397", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "KALINICHENKO, Lina P"}], "nameIdentifiers": [{"nameIdentifier": "2398", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "ORLOV, Nikolay Y"}], "nameIdentifiers": [{"nameIdentifier": "2399", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "KUWAJIMA, Kunihiro "}], "nameIdentifiers": [{"nameIdentifier": "2361", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "SUGAI, Shintaro "}], "nameIdentifiers": [{"nameIdentifier": "2363", "nameIdentifierScheme": "WEKO"}]}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Calcium-regulated interactions of human α-lactalbumin with bee venom melittin", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Calcium-regulated interactions of human α-lactalbumin with bee venom melittin"}, {"subitem_title": "Calcium-regulated interactions of human α-lactalbumin with bee venom melittin", "subitem_title_language": "en"}]}, "item_type_id": "10001", "owner": "21", "path": ["323"], "permalink_uri": "https://ir.soken.ac.jp/records/4146", "pubdate": {"attribute_name": "公開日", "attribute_value": "2014-01-15"}, "publish_date": "2014-01-15", "publish_status": "0", "recid": "4146", "relation": {}, "relation_version_is_last": true, "title": ["Calcium-regulated interactions of human α-lactalbumin with bee venom melittin"], "weko_shared_id": 21}