| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2014-01-15 |
| タイトル |
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タイトル |
Calcium-regulated interactions of human α-lactalbumin with bee venom melittin |
| タイトル |
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タイトル |
Calcium-regulated interactions of human α-lactalbumin with bee venom melittin |
|
言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
metadata only access |
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アクセス権URI |
http://purl.org/coar/access_right/c_14cb |
| 著者 |
PERMYAKOV, Eugene A
GRISHCHENKO, Valery M
KALINICHENKO, Lina P
ORLOV, Nikolay Y
KUWAJIMA, Kunihiro
SUGAI, Shintaro
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| 著者別名 |
桑島, 邦博
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
Affinity chromatography, fluorescence and circular dichroism spectroscopy methods have been used to study the interaction of melittin, a 26-residue peptide from bee venom, with Ca2+-binding α-lactalbumin from human milk. It has been revealed that melittin binds to the apo- and acidic states of α-lactalbumin while the presence of Ca2+ makes the interaction essentially weaker. The association constant for the complex of melittin with apo-α-lactalbumin determined from spectropolarimetric melittin-titration data is 2×107 M−1. The complexation of α-lactalbumin with melittin decreases its affinity to Ca2+ by three orders of magnitude. The interaction of apo-α-lactalbumin with melittin causes some changes in the environment of its aromatic amino acid residues and drastically alters the conformation of melittin, increasing its α-helical content but leaving its single tryptophan residue accessible to water. In the case of the acidic state of α-lactalbumin the interaction does not induce an increase in α-helical content of melittin. |
| 書誌情報 |
Biophysical Chemistry
en : Biophysical Chemistry
巻 39,
号 2,
p. 111-117,
発行日 1991-02
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| 出版者 |
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出版者 |
Elsevier |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0301-4622 |
| PubMed番号 |
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識別子タイプ |
PMID |
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関連識別子 |
2059662 |
| DOI |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1016/0301-4622(91)85012-F |
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関連名称 |
10.1016/0301-4622(91)85012-F |
| 権利 |
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権利情報 |
© 1991 Published by Elsevier B.V. |
