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In contrast to the biphasic unfolding of the wild-type nuclease, the unfolding of the mutant is represented by a single-phase reaction, indicating that the biphasic unfolding for the wild-type protein is caused by cis-trans isomerization about the prolyl peptide bond in the native state. The proline mutation also simplifies the kinetic refolding. Importance of the results in elucidating the folding mechanism is discussed.", "subitem_description_type": "Abstract"}]}, "item_10001_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Elsevier "}]}, "item_10001_relation_13": {"attribute_name": "PubMed番号", "attribute_value_mlt": [{"subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "1915864", "subitem_relation_type_select": "PMID"}}]}, "item_10001_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1016/0014-5793(91)81243-2"}], "subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1016/0014-5793(91)81243-2", "subitem_relation_type_select": "DOI"}}]}, "item_10001_relation_17": {"attribute_name": "関連サイト", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "Copyright"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "http://www.elsevier.com/journal-authors/author-rights-and-responsibilities", "subitem_relation_type_select": "URI"}}]}, "item_10001_rights_15": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "© 1991 Published by Elsevier B.V."}]}, "item_10001_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0014-5793 ", "subitem_source_identifier_type": "ISSN"}]}, "item_10001_version_type_20": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "KUWAJIMA, Kunihiro "}], "nameIdentifiers": [{"nameIdentifier": "2361", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "OKAYAMA, Naoki "}], "nameIdentifiers": [{"nameIdentifier": "2394", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "YAMAMOTO, Kaori "}], "nameIdentifiers": [{"nameIdentifier": "2395", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "ISHIHARA, Tsuyoshi "}], "nameIdentifiers": [{"nameIdentifier": "2396", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "SUGAI, Shintaro "}], "nameIdentifiers": [{"nameIdentifier": "2363", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2014-01-15"}], "displaytype": "simple", "download_preview_message": "", "file_order": 0, "filename": "1-s2.0-0014579391812432-main.pdf", "filesize": [{"value": "490.3 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 490300.0, "url": {"label": "1-s2.0-0014579391812432-main", "url": "https://ir.soken.ac.jp/record/4147/files/1-s2.0-0014579391812432-main.pdf"}, "version_id": "e4535a45-369a-4316-b1ca-6b585c76fddd"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "The pro117 to glycine mutation of staphylococcal nuclease simplifies the unfolding folding kinetics", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "The pro117 to glycine mutation of staphylococcal nuclease simplifies the unfolding folding kinetics"}, {"subitem_title": "The pro117 to glycine mutation of staphylococcal nuclease simplifies the unfolding folding kinetics", "subitem_title_language": "en"}]}, "item_type_id": "10001", "owner": "21", "path": ["323"], "permalink_uri": "https://ir.soken.ac.jp/records/4147", "pubdate": {"attribute_name": "公開日", "attribute_value": "2014-01-15"}, "publish_date": "2014-01-15", "publish_status": "0", "recid": "4147", "relation": {}, "relation_version_is_last": true, "title": ["The pro117 to glycine mutation of staphylococcal nuclease simplifies the unfolding folding kinetics"], "weko_shared_id": 21}