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The pro117 to glycine mutation of staphylococcal nuclease simplifies the unfolding folding kinetics
https://ir.soken.ac.jp/records/4147
https://ir.soken.ac.jp/records/4147e0f5fde6-6b14-4a77-81d1-de3a46c9e823
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
1-s2.0-0014579391812432-main (490.3 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2014-01-15 | |||||
| タイトル | ||||||
| タイトル | The pro117 to glycine mutation of staphylococcal nuclease simplifies the unfolding folding kinetics | |||||
| タイトル | ||||||
| タイトル | The pro117 to glycine mutation of staphylococcal nuclease simplifies the unfolding folding kinetics | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
KUWAJIMA, Kunihiro
× KUWAJIMA, Kunihiro× OKAYAMA, Naoki× YAMAMOTO, Kaori× ISHIHARA, Tsuyoshi× SUGAI, Shintaro |
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| 著者別名 |
桑島, 邦博
× 桑島, 邦博 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Kinetics of unfolding and refolding of a staphylococcal nuclease mutant, in which Pro117 is replaced by glycine, have been investigated by stopped-flow circular dichroism, and the results are compared with those for the wild-type protein. In contrast to the biphasic unfolding of the wild-type nuclease, the unfolding of the mutant is represented by a single-phase reaction, indicating that the biphasic unfolding for the wild-type protein is caused by cis-trans isomerization about the prolyl peptide bond in the native state. The proline mutation also simplifies the kinetic refolding. Importance of the results in elucidating the folding mechanism is discussed. | |||||
| 書誌情報 |
FEBS Letters en : FEBS Letters 巻 290, 号 1-2, p. 135-138, 発行日 1991-09-23 |
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| 出版者 | ||||||
| 出版者 | Elsevier | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0014-5793 | |||||
| PubMed番号 | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | PMID | |||||
| 関連識別子 | 1915864 | |||||
| DOI | ||||||
| 関連タイプ | isIdenticalTo | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1016/0014-5793(91)81243-2 | |||||
| 関連名称 | 10.1016/0014-5793(91)81243-2 | |||||
| 権利 | ||||||
| 権利情報 | © 1991 Published by Elsevier B.V. | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://www.elsevier.com/journal-authors/author-rights-and-responsibilities | |||||
| 関連名称 | Copyright | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
| 著者版フラグ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
