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The pro117 to glycine mutation of staphylococcal nuclease simplifies the unfolding folding kinetics
https://ir.soken.ac.jp/records/4147
https://ir.soken.ac.jp/records/4147e0f5fde6-6b14-4a77-81d1-de3a46c9e823
名前 / ファイル | ライセンス | アクション |
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2014-01-15 | |||||
タイトル | ||||||
タイトル | The pro117 to glycine mutation of staphylococcal nuclease simplifies the unfolding folding kinetics | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | The pro117 to glycine mutation of staphylococcal nuclease simplifies the unfolding folding kinetics | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
KUWAJIMA, Kunihiro
× KUWAJIMA, Kunihiro× OKAYAMA, Naoki× YAMAMOTO, Kaori× ISHIHARA, Tsuyoshi× SUGAI, Shintaro |
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著者別名 |
桑島, 邦博
× 桑島, 邦博 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Kinetics of unfolding and refolding of a staphylococcal nuclease mutant, in which Pro117 is replaced by glycine, have been investigated by stopped-flow circular dichroism, and the results are compared with those for the wild-type protein. In contrast to the biphasic unfolding of the wild-type nuclease, the unfolding of the mutant is represented by a single-phase reaction, indicating that the biphasic unfolding for the wild-type protein is caused by cis-trans isomerization about the prolyl peptide bond in the native state. The proline mutation also simplifies the kinetic refolding. Importance of the results in elucidating the folding mechanism is discussed. | |||||
書誌情報 |
FEBS Letters en : FEBS Letters 巻 290, 号 1-2, p. 135-138, 発行日 1991-09-23 |
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出版者 | ||||||
出版者 | Elsevier | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0014-5793 | |||||
PubMed番号 | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | PMID | |||||
関連識別子 | 1915864 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1016/0014-5793(91)81243-2 | |||||
関連名称 | 10.1016/0014-5793(91)81243-2 | |||||
権利 | ||||||
権利情報 | © 1991 Published by Elsevier B.V. | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | http://www.elsevier.com/journal-authors/author-rights-and-responsibilities | |||||
関連名称 | Copyright | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |