| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2014-01-17 |
| タイトル |
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タイトル |
The chaperonin GroEL does not recognize apo--lactalbumin in the molten globule state |
| タイトル |
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タイトル |
The chaperonin GroEL does not recognize apo--lactalbumin in the molten globule state |
|
言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
metadata only access |
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アクセス権URI |
http://purl.org/coar/access_right/c_14cb |
| 著者 |
OKAZAKI, Akira
IKURA, Teikichi
NIKAIDO, Kiyokazu
KUWAJIMA, Kunihiro
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| 著者別名 |
桑島, 邦博
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
We investigate here the interaction between GroEL and two kinds of non-native -lactalbumin. -Lactalbumin is a Ca2+-binding protein which assumes a molten globule state in the absence of Ca2+ (apo--lactalbumin) at neutral pH. Our results, obtained by molecular-sieve chromatography and hydrogen-exchange measurements, show that apo--lactalbumin in this molten globule state is not bound to GroEL either in the absence or in the presence of KCl. On the other hand, we show by molecular-sieve chromatography that -lactalbumin, in which the four disulphide bonds are fully reduced, is bound to GroEL when 50 mM KCl is present. The results demonstrate that the protein state recognized by GroEL is more unfolded and expanded than the typical molten globule state of -lactalbumin. |
| 書誌情報 |
Nature Structural Biology
en : Nature Structural Biology
巻 1,
p. 439-446,
発行日 1994
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| 出版者 |
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出版者 |
Nature Publishing Group |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
1545-9993 |
| DOI |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1038/nsb0794-439 |
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関連名称 |
10.1038/nsb0794-439 |
| 権利 |
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権利情報 |
©1994 Nature Publishing Group |
