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Partial characterization and light-induced regulation of GTP-binding proteins in Lemna paucicostata.
https://ir.soken.ac.jp/records/4226
https://ir.soken.ac.jp/records/4226f1753342-c5d7-4ae8-a686-e344e6f93c99
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2014-02-10 | |||||
| タイトル | ||||||
| タイトル | Partial characterization and light-induced regulation of GTP-binding proteins in Lemna paucicostata. | |||||
| タイトル | ||||||
| タイトル | Partial characterization and light-induced regulation of GTP-binding proteins in Lemna paucicostata. | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
HASUNUMA, Kohji
× HASUNUMA, Kohji× FURUKAWA, Kazuhiko× FUNADERA, Kanako× KUBOTA, Mamoru× WATANABE, Masakatsu |
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| 著者別名 |
渡辺, 正勝
× 渡辺, 正勝 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Binding of GTP-binding proteins with [35S]GTP7S in the extract containing membrane components of Lemna paucicostata 441 was inhibited by red or far red light by 20 to 25%, but blue light showed no or little effect. The plant used for the preparation of the extract was subjected to single darkness for 8 h, as both red and far red light inhibit flowering. The extract treated with 1% Lubrol was fractionated by gel filtration. Four species of GTP-binding proteins, GL1, GL2, GL3 and GL4 were detected with Km values 3, 7, 80 and 4 nM, respectively. GL1, GL2 and GL3 were ADP-ribosylated by pertussis toxin. The extract activated by [35S]GTP-γS in darkness, under red light or under far red light was treated with 1% Lubrol and subsequent gel filtration of the extracts made it possible to detect GTP-binding protein with a small molecular weight only in an extract labeled in darkness. The reduction in the molecular weight of GTP-binding protein from the larger molecule associated with the binding of [35S]GTPγS was confirmed by rechromatography of the larger molecule activated by [35S]GTPγS in darkness. The binding of GL2 and/or GL3 with [35S]GTPγS was suggested to be inhibited by red or far red light. | |||||
| 書誌情報 |
Photochemistry and Photobiology en : Photochemistry and Photobiology 巻 46, 号 4, p. 531-535, 発行日 1987-10 |
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| 出版者 | ||||||
| 出版者 | Wiley | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0031-8655 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1111/j.1751-1097.1987.tb04807.x | |||||
| 関連名称 | 10.1111/j.1751-1097.1987.tb04807.x | |||||
| 権利 | ||||||
| 権利情報 | © 1999-2014 John Wiley & Sons, Inc. | |||||
