| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2014-02-10 |
| タイトル |
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タイトル |
Partial characterization and light-induced regulation of GTP-binding proteins in Lemna paucicostata. |
| タイトル |
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タイトル |
Partial characterization and light-induced regulation of GTP-binding proteins in Lemna paucicostata. |
|
言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
metadata only access |
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アクセス権URI |
http://purl.org/coar/access_right/c_14cb |
| 著者 |
HASUNUMA, Kohji
FURUKAWA, Kazuhiko
FUNADERA, Kanako
KUBOTA, Mamoru
WATANABE, Masakatsu
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| 著者別名 |
渡辺, 正勝
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| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
Binding of GTP-binding proteins with [35S]GTP7S in the extract containing membrane components of Lemna paucicostata 441 was inhibited by red or far red light by 20 to 25%, but blue light showed no or little effect. The plant used for the preparation of the extract was subjected to single darkness for 8 h, as both red and far red light inhibit flowering. The extract treated with 1% Lubrol was fractionated by gel filtration. Four species of GTP-binding proteins, GL1, GL2, GL3 and GL4 were detected with Km values 3, 7, 80 and 4 nM, respectively. GL1, GL2 and GL3 were ADP-ribosylated by pertussis toxin. The extract activated by [35S]GTP-γS in darkness, under red light or under far red light was treated with 1% Lubrol and subsequent gel filtration of the extracts made it possible to detect GTP-binding protein with a small molecular weight only in an extract labeled in darkness. The reduction in the molecular weight of GTP-binding protein from the larger molecule associated with the binding of [35S]GTPγS was confirmed by rechromatography of the larger molecule activated by [35S]GTPγS in darkness. The binding of GL2 and/or GL3 with [35S]GTPγS was suggested to be inhibited by red or far red light. |
| 書誌情報 |
Photochemistry and Photobiology
en : Photochemistry and Photobiology
巻 46,
号 4,
p. 531-535,
発行日 1987-10
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| 出版者 |
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出版者 |
Wiley |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0031-8655 |
| DOI |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1111/j.1751-1097.1987.tb04807.x |
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関連名称 |
10.1111/j.1751-1097.1987.tb04807.x |
| 権利 |
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権利情報 |
© 1999-2014 John Wiley & Sons, Inc. |
