WEKO3
アイテム
The use of the time-resolved X-ray solution scattering for studies of globular proteins
https://ir.soken.ac.jp/records/4306
https://ir.soken.ac.jp/records/4306f0bd9bba-fe2b-4028-b587-1f730ad4f411
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2014-03-17 | |||||
| タイトル | ||||||
| タイトル | The use of the time-resolved X-ray solution scattering for studies of globular proteins | |||||
| タイトル | ||||||
| タイトル | The use of the time-resolved X-ray solution scattering for studies of globular proteins | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
KUWAJIMA, Kunihiro
× KUWAJIMA, Kunihiro× ARAI, Munehito× et, al. |
|||||
| 著者別名 |
桑島, 邦博
× 桑島, 邦博 |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | In order to improve the low signal-to-noise ratio of the time-resolved small-angle X-ray scattering, we have used a two-dimensional X-ray detector with a beryllium-windowed X-ray image intensifier and a charge-coupled device as an image sensor, and applied this to studies on (1) the kinetic folding reaction of α-lactalbumin, which accumulates the molten globule-like intermediate at an early stage of refolding and (2) the cooperative conformational transition of Escherichia coli chaperonin GroEL induced by ATP, which occurs in an allosteric manner between the close and open conformational states. In the α-lactalbumin reaction, we have firmly established the equivalence between the kinetic intermediate and the equilibrium molten globule state, and obtained further information about dehydration from the highly hydrated folding intermediate during a late stage of refolding. In the chaperonin study, we have successfully observed the kinetics of the allosteric transition of GroEL that occurs with a rate constant of about 3–4 s-1 at 5°C. The combination of the time-resolved X-ray scattering with other spectroscopic techniques such as circular dichroism and intrinsic fluorescence is thus very effective in understanding the conformational transitions of proteins and protein complexes. | |||||
| 書誌情報 |
Spectroscopy: An International Journal en : Spectroscopy: An International Journal 巻 16, 号 3-4, p. 127-138, 発行日 2002 |
|||||
| 出版者 | ||||||
| 出版者 | IOS Press | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0712-4813 | |||||
| 権利 | ||||||
| 権利情報 | ©2002 IOS Press | |||||
