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Parallel folding pathway of proline-free staphylococcal nuclease studied by the stopped-flow double-jump method
https://ir.soken.ac.jp/records/4310
https://ir.soken.ac.jp/records/43104142bd37-1900-4e9f-8406-2ed012f48fed
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2014-03-18 | |||||
| タイトル | ||||||
| タイトル | Parallel folding pathway of proline-free staphylococcal nuclease studied by the stopped-flow double-jump method | |||||
| タイトル | ||||||
| タイトル | Parallel folding pathway of proline-free staphylococcal nuclease studied by the stopped-flow double-jump method | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
KAMAGATA, Kiyoto
× KAMAGATA, Kiyoto× KUWAJIMA, Kunihiro |
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| 著者別名 |
桑島, 邦博
× 桑島, 邦博 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The folding mechanism of proline-free staphylococcal nuclease (SNase (pro-)) (P11A, P31A, P42A, P47T, P56A, P117G) was investigated using the double-jump stopped-flow method (interrupted refolding). This method has enabled us to specifically monitor the amount of the native molecules during the refolding. The results indicate that the middle and slow phases observed in the refolding kinetics represent the formation of the native state (IM → N, IS → N) and that the folding mechanism of SNase (pro-) is not represented by a single sequential pathway, but at least two parallel pathways are required for interpreting the results. | |||||
| 書誌情報 |
Spectroscopy: An International Journal en : Spectroscopy: An International Journal 巻 17, 号 2-3, p. 203-212, 発行日 2003 |
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| 出版者 | ||||||
| 出版者 | IOS Press | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0712-4813 | |||||
| 権利 | ||||||
| 権利情報 | ©2003 IOS Press | |||||
| 関連サイト | ||||||
| 識別子タイプ | URI | |||||
| 関連識別子 | https://content.iospress.com/articles/spectroscopy/spe097 | |||||
| 関連名称 | IOS Press | |||||
