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Reversible and Fast Association Equilibria of a Molecular Chaperone, gp57A, of Bacteriophage T4
https://ir.soken.ac.jp/records/4315
https://ir.soken.ac.jp/records/43154a70b09e-ef38-4f2a-8a22-520ca6b7b681
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
1-s2.0-S0006349503746839-main (316.6 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||
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| 公開日 | 2014-03-19 | |||||||||||
| タイトル | ||||||||||||
| タイトル | Reversible and Fast Association Equilibria of a Molecular Chaperone, gp57A, of Bacteriophage T4 | |||||||||||
| タイトル | ||||||||||||
| タイトル | Reversible and Fast Association Equilibria of a Molecular Chaperone, gp57A, of Bacteriophage T4 | |||||||||||
| 言語 | en | |||||||||||
| 言語 | ||||||||||||
| 言語 | eng | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
| 資源タイプ | journal article | |||||||||||
| 著者 |
ALI, Said A.
× ALI, Said A.
× KUWAJIMA, Kunihiro
× et, al.
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| 著者別名 |
桑島, 邦博
× 桑島, 邦博
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| 抄録 | ||||||||||||
| 内容記述タイプ | Abstract | |||||||||||
| 内容記述 | The association of a molecular chaperone, gp57A, of bacteriophage T4, which facilitates formation of the long and short tail fibers, was investigated by analytical ultracentrifugation, differential scanning microcalorimetry, and stopped-flow circular dichroism (CD) to establish the association scheme of the protein. Gp57A is an oligomeric α-helix protein with 79 amino acids. Analysis of the sedimentation velocity data by direct boundary modeling with Lamm equation solutions together with a more detailed boundary analysis incorporating association schemes led us to conclude that at least three oligomeric species of gp57A are in reversible and fast association equilibria and that a 3mer-6mer-12mer model described the data best. On the other hand, differential scanning microcalorimetry revealed a highly reversible two-step transition of dissociation/denaturation, both of which accompanied decrease in CD at 222 nm. The melting curve analysis revealed that it is consistent with a 6mer-3mer-1mer model. The refolding/association kinetics of gp57A measured by stopped-flow CD was consistent with the interpretation that the bimolecular reaction from trimer to hexamer was preceded by a fast α-helix formation in the dead-time. Trimer or hexamer is likely the functional oligomeric state of gp57A. | |||||||||||
| 書誌情報 |
Biophysical Journal en : Biophysical Journal 巻 85, 号 4, p. 2606-2618, 発行日 2003-10 |
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| 出版者 | ||||||||||||
| 出版者 | Elsevier | |||||||||||
| ISSN | ||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||
| 収録物識別子 | 0006-3495 | |||||||||||
| DOI | ||||||||||||
| 関連タイプ | isIdenticalTo | |||||||||||
| 識別子タイプ | DOI | |||||||||||
| 関連識別子 | https://doi.org/10.1016/S0006-3495(03)74683-9 | |||||||||||
| 関連名称 | 10.1016/S0006-3495(03)74683-9 | |||||||||||
| 権利 | ||||||||||||
| 権利情報 | © 2003 The Biophysical Society. | |||||||||||
| 関連サイト | ||||||||||||
| 識別子タイプ | URI | |||||||||||
| 関連識別子 | http://download.cell.com/images/edimages/Biophys/Instructions_to_Authors.pdf | |||||||||||
| 関連名称 | Copyright | |||||||||||
| フォーマット | ||||||||||||
| 内容記述タイプ | Other | |||||||||||
| 内容記述 | application/pdf | |||||||||||
| 著者版フラグ | ||||||||||||
| 出版タイプ | VoR | |||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||
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ALI, Said A. , KUWAJIMA, Kunihiro , et, al., 2003, Reversible and Fast Association Equilibria of a Molecular Chaperone, gp57A, of Bacteriophage T4: Elsevier, 2606–2618 p.
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