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  1. 010 学術雑誌論文
  2. 桑島, 邦博 / KUWAJIMA, Kunihiro

Reversible and Fast Association Equilibria of a Molecular Chaperone, gp57A, of Bacteriophage T4

https://ir.soken.ac.jp/records/4315
https://ir.soken.ac.jp/records/4315
4a70b09e-ef38-4f2a-8a22-520ca6b7b681
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1-s2.0-S0006349503746839-main.pdf 1-s2.0-S0006349503746839-main (316.6 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2014-03-19
タイトル
タイトル Reversible and Fast Association Equilibria of a Molecular Chaperone, gp57A, of Bacteriophage T4
タイトル
タイトル Reversible and Fast Association Equilibria of a Molecular Chaperone, gp57A, of Bacteriophage T4
言語 en
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ journal article
著者 ALI, Said A.

× ALI, Said A.

ALI, Said A.

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KUWAJIMA, Kunihiro

× KUWAJIMA, Kunihiro

KUWAJIMA, Kunihiro

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et, al.

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et, al.

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著者別名 桑島, 邦博

× 桑島, 邦博

桑島, 邦博

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抄録
内容記述タイプ Abstract
内容記述 The association of a molecular chaperone, gp57A, of bacteriophage T4, which facilitates formation of the long and short tail fibers, was investigated by analytical ultracentrifugation, differential scanning microcalorimetry, and stopped-flow circular dichroism (CD) to establish the association scheme of the protein. Gp57A is an oligomeric α-helix protein with 79 amino acids. Analysis of the sedimentation velocity data by direct boundary modeling with Lamm equation solutions together with a more detailed boundary analysis incorporating association schemes led us to conclude that at least three oligomeric species of gp57A are in reversible and fast association equilibria and that a 3mer-6mer-12mer model described the data best. On the other hand, differential scanning microcalorimetry revealed a highly reversible two-step transition of dissociation/denaturation, both of which accompanied decrease in CD at 222 nm. The melting curve analysis revealed that it is consistent with a 6mer-3mer-1mer model. The refolding/association kinetics of gp57A measured by stopped-flow CD was consistent with the interpretation that the bimolecular reaction from trimer to hexamer was preceded by a fast α-helix formation in the dead-time. Trimer or hexamer is likely the functional oligomeric state of gp57A.
書誌情報 Biophysical Journal
en : Biophysical Journal

巻 85, 号 4, p. 2606-2618, 発行日 2003-10
出版者
出版者 Elsevier
ISSN
収録物識別子タイプ ISSN
収録物識別子 0006-3495
DOI
関連タイプ isIdenticalTo
識別子タイプ DOI
関連識別子 https://doi.org/10.1016/S0006-3495(03)74683-9
関連名称 10.1016/S0006-3495(03)74683-9
権利
権利情報 © 2003 The Biophysical Society.
関連サイト
識別子タイプ URI
関連識別子 http://download.cell.com/images/edimages/Biophys/Instructions_to_Authors.pdf
関連名称 Copyright
フォーマット
内容記述タイプ Other
内容記述 application/pdf
著者版フラグ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
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