| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2014-03-19 |
| タイトル |
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タイトル |
Reversible and Fast Association Equilibria of a Molecular Chaperone, gp57A, of Bacteriophage T4 |
| タイトル |
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タイトル |
Reversible and Fast Association Equilibria of a Molecular Chaperone, gp57A, of Bacteriophage T4 |
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言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| 著者 |
ALI, Said A.
KUWAJIMA, Kunihiro
et, al.
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| 著者別名 |
桑島, 邦博
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| 抄録 |
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内容記述タイプ |
Abstract |
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内容記述 |
The association of a molecular chaperone, gp57A, of bacteriophage T4, which facilitates formation of the long and short tail fibers, was investigated by analytical ultracentrifugation, differential scanning microcalorimetry, and stopped-flow circular dichroism (CD) to establish the association scheme of the protein. Gp57A is an oligomeric α-helix protein with 79 amino acids. Analysis of the sedimentation velocity data by direct boundary modeling with Lamm equation solutions together with a more detailed boundary analysis incorporating association schemes led us to conclude that at least three oligomeric species of gp57A are in reversible and fast association equilibria and that a 3mer-6mer-12mer model described the data best. On the other hand, differential scanning microcalorimetry revealed a highly reversible two-step transition of dissociation/denaturation, both of which accompanied decrease in CD at 222 nm. The melting curve analysis revealed that it is consistent with a 6mer-3mer-1mer model. The refolding/association kinetics of gp57A measured by stopped-flow CD was consistent with the interpretation that the bimolecular reaction from trimer to hexamer was preceded by a fast α-helix formation in the dead-time. Trimer or hexamer is likely the functional oligomeric state of gp57A. |
| 書誌情報 |
Biophysical Journal
en : Biophysical Journal
巻 85,
号 4,
p. 2606-2618,
発行日 2003-10
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| 出版者 |
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出版者 |
Elsevier |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
0006-3495 |
| DOI |
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関連タイプ |
isIdenticalTo |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1016/S0006-3495(03)74683-9 |
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関連名称 |
10.1016/S0006-3495(03)74683-9 |
| 権利 |
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権利情報 |
© 2003 The Biophysical Society. |
| 関連サイト |
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識別子タイプ |
URI |
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関連識別子 |
http://download.cell.com/images/edimages/Biophys/Instructions_to_Authors.pdf |
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関連名称 |
Copyright |
| フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
| 著者版フラグ |
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出版タイプ |
VoR |
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出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
1-s2.0-S0006349503746839-main (316.6 kB)
