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Reversible and Fast Association Equilibria of a Molecular Chaperone, gp57A, of Bacteriophage T4
https://ir.soken.ac.jp/records/4315
https://ir.soken.ac.jp/records/43154a70b09e-ef38-4f2a-8a22-520ca6b7b681
名前 / ファイル | ライセンス | アクション |
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2014-03-19 | |||||
タイトル | ||||||
タイトル | Reversible and Fast Association Equilibria of a Molecular Chaperone, gp57A, of Bacteriophage T4 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Reversible and Fast Association Equilibria of a Molecular Chaperone, gp57A, of Bacteriophage T4 | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
ALI, Said A.
× ALI, Said A.× KUWAJIMA, Kunihiro× et, al. |
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著者別名 |
桑島, 邦博
× 桑島, 邦博 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The association of a molecular chaperone, gp57A, of bacteriophage T4, which facilitates formation of the long and short tail fibers, was investigated by analytical ultracentrifugation, differential scanning microcalorimetry, and stopped-flow circular dichroism (CD) to establish the association scheme of the protein. Gp57A is an oligomeric α-helix protein with 79 amino acids. Analysis of the sedimentation velocity data by direct boundary modeling with Lamm equation solutions together with a more detailed boundary analysis incorporating association schemes led us to conclude that at least three oligomeric species of gp57A are in reversible and fast association equilibria and that a 3mer-6mer-12mer model described the data best. On the other hand, differential scanning microcalorimetry revealed a highly reversible two-step transition of dissociation/denaturation, both of which accompanied decrease in CD at 222 nm. The melting curve analysis revealed that it is consistent with a 6mer-3mer-1mer model. The refolding/association kinetics of gp57A measured by stopped-flow CD was consistent with the interpretation that the bimolecular reaction from trimer to hexamer was preceded by a fast α-helix formation in the dead-time. Trimer or hexamer is likely the functional oligomeric state of gp57A. | |||||
書誌情報 |
Biophysical Journal en : Biophysical Journal 巻 85, 号 4, p. 2606-2618, 発行日 2003-10 |
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出版者 | ||||||
出版者 | Elsevier | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0006-3495 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1016/S0006-3495(03)74683-9 | |||||
関連名称 | 10.1016/S0006-3495(03)74683-9 | |||||
権利 | ||||||
権利情報 | © 2003 The Biophysical Society. | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | http://download.cell.com/images/edimages/Biophys/Instructions_to_Authors.pdf | |||||
関連名称 | Copyright | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |