{"_buckets": {"deposit": "a1c93d23-6d70-4a94-a4be-2eb90090a346"}, "_deposit": {"created_by": 21, "id": "4315", "owners": [21], "pid": {"revision_id": 0, "type": "depid", "value": "4315"}, "status": "published"}, "_oai": {"id": "oai:ir.soken.ac.jp:00004315", "sets": ["323"]}, "author_link": ["2364", "2361", "3119", "2519"], "item_10001_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2003-10", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "4", "bibliographicPageEnd": "2618", "bibliographicPageStart": "2606", "bibliographicVolumeNumber": "85", "bibliographic_titles": [{"bibliographic_title": "Biophysical Journal"}, {"bibliographic_title": "Biophysical Journal", "bibliographic_titleLang": "en"}]}]}, "item_10001_creator_3": {"attribute_name": "著者別名", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "桑島, 邦博"}], "nameIdentifiers": [{"nameIdentifier": "2364", "nameIdentifierScheme": "WEKO"}, {"nameIdentifier": "1000070091444", "nameIdentifierScheme": "NRID", "nameIdentifierURI": " "}, {"nameIdentifier": "70091444", "nameIdentifierScheme": "e-Rad", "nameIdentifierURI": "https://kaken.nii.ac.jp/ja/search/?qm=70091444"}]}]}, "item_10001_description_19": {"attribute_name": "フォーマット", "attribute_value_mlt": [{"subitem_description": "application/pdf", "subitem_description_type": "Other"}]}, "item_10001_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "The association of a molecular chaperone, gp57A, of bacteriophage T4, which facilitates formation of the long and short tail fibers, was investigated by analytical ultracentrifugation, differential scanning microcalorimetry, and stopped-flow circular dichroism (CD) to establish the association scheme of the protein. Gp57A is an oligomeric α-helix protein with 79 amino acids. Analysis of the sedimentation velocity data by direct boundary modeling with Lamm equation solutions together with a more detailed boundary analysis incorporating association schemes led us to conclude that at least three oligomeric species of gp57A are in reversible and fast association equilibria and that a 3mer-6mer-12mer model described the data best. On the other hand, differential scanning microcalorimetry revealed a highly reversible two-step transition of dissociation/denaturation, both of which accompanied decrease in CD at 222 nm. The melting curve analysis revealed that it is consistent with a 6mer-3mer-1mer model. The refolding/association kinetics of gp57A measured by stopped-flow CD was consistent with the interpretation that the bimolecular reaction from trimer to hexamer was preceded by a fast α-helix formation in the dead-time. Trimer or hexamer is likely the functional oligomeric state of gp57A.", "subitem_description_type": "Abstract"}]}, "item_10001_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Elsevier"}]}, "item_10001_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1016/S0006-3495(03)74683-9"}], "subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1016/S0006-3495(03)74683-9", "subitem_relation_type_select": "DOI"}}]}, "item_10001_relation_17": {"attribute_name": "関連サイト", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "Copyright"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "http://download.cell.com/images/edimages/Biophys/Instructions_to_Authors.pdf", "subitem_relation_type_select": "URI"}}]}, "item_10001_rights_15": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "© 2003 The Biophysical Society."}]}, "item_10001_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0006-3495", "subitem_source_identifier_type": "ISSN"}]}, "item_10001_version_type_20": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "ALI, Said A. "}], "nameIdentifiers": [{"nameIdentifier": "2519", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "KUWAJIMA, Kunihiro "}], "nameIdentifiers": [{"nameIdentifier": "2361", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "et, al."}], "nameIdentifiers": [{"nameIdentifier": "3119", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2014-03-19"}], "displaytype": "simple", "download_preview_message": "", "file_order": 0, "filename": "1-s2.0-S0006349503746839-main.pdf", "filesize": [{"value": "316.6 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 316600.0, "url": {"label": "1-s2.0-S0006349503746839-main", "url": "https://ir.soken.ac.jp/record/4315/files/1-s2.0-S0006349503746839-main.pdf"}, "version_id": "5111e3c7-0922-4811-a8d5-2b633deb6eca"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Reversible and Fast Association Equilibria of a Molecular Chaperone, gp57A, of Bacteriophage T4", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Reversible and Fast Association Equilibria of a Molecular Chaperone, gp57A, of Bacteriophage T4"}, {"subitem_title": "Reversible and Fast Association Equilibria of a Molecular Chaperone, gp57A, of Bacteriophage T4", "subitem_title_language": "en"}]}, "item_type_id": "10001", "owner": "21", "path": ["323"], "permalink_uri": "https://ir.soken.ac.jp/records/4315", "pubdate": {"attribute_name": "公開日", "attribute_value": "2014-03-19"}, "publish_date": "2014-03-19", "publish_status": "0", "recid": "4315", "relation": {}, "relation_version_is_last": true, "title": ["Reversible and Fast Association Equilibria of a Molecular Chaperone, gp57A, of Bacteriophage T4"], "weko_shared_id": 21}