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Atomically Detailed Description of the Unfolding of α-Lactalbumin by the Combined Use of Experiments and Simulations
https://ir.soken.ac.jp/records/4324
https://ir.soken.ac.jp/records/43248cfb7cbe-9dc0-4250-988a-6bf1d15faabe
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2014-03-24 | |||||
| タイトル | ||||||
| タイトル | Atomically Detailed Description of the Unfolding of α-Lactalbumin by the Combined Use of Experiments and Simulations | |||||
| タイトル | ||||||
| タイトル | Atomically Detailed Description of the Unfolding of α-Lactalbumin by the Combined Use of Experiments and Simulations | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
OROGUCHI, Tomotaka
× OROGUCHI, Tomotaka× KUWAJIMA, Kunihiro× et, al. |
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| 著者別名 |
桑島, 邦博
× 桑島, 邦博 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The recombinant form of goat α-lactalbumin has a significantly faster unfolding rate compared to the authentic form, although the two molecules differ only in an extra methionine at the N terminus of the recombinant. The mechanism of the destabilization caused by this residue was investigated through the combined use of kinetic experiments and molecular dynamics simulations. Unfolding simulations for the authentic and recombinant forms at 398 K (ten trajectories of 5 ns for each form, 100 ns total) precisely reproduced the experimentally observed differences in unfolding behavior. In addition, experiments reproduced the destabilization of a mutant protein, T38A, faithfully as predicted by the simulations. This bidirectional verification between experiments and simulations enabled the atomically detailed description of the role of the extra methionine residue in the unfolding process. | |||||
| 書誌情報 |
Journal of Molecular Biology en : Journal of Molecular Biology 巻 354, 号 1, p. 164-172, 発行日 2005-11-18 |
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| 出版者 | ||||||
| 出版者 | Elsevier | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0022-2836 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1016/j.jmb.2005.09.061 | |||||
| 関連名称 | 10.1016/j.jmb.2005.09.061 | |||||
| 権利 | ||||||
| 権利情報 | © 2005 Elsevier Ltd. | |||||
