{"_buckets": {"deposit": "ee3b1774-b377-447a-96d7-4be726e797ae"}, "_deposit": {"created_by": 1, "id": "946", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "946"}, "status": "published"}, "_oai": {"id": "oai:ir.soken.ac.jp:00000946", "sets": ["20"]}, "author_link": ["9782", "9781", "9780"], "item_1_biblio_info_21": {"attribute_name": "書誌情報（ソート用）", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1999-03-24", "bibliographicIssueDateType": "Issued"}, "bibliographic_titles": [{}]}]}, "item_1_creator_2": {"attribute_name": "著者名", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "寺社下, 美樹"}], "nameIdentifiers": [{"nameIdentifier": "9780", "nameIdentifierScheme": "WEKO"}]}]}, "item_1_creator_3": {"attribute_name": "フリガナ", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "ジシャゲ, ミキ"}], "nameIdentifiers": [{"nameIdentifier": "9781", "nameIdentifierScheme": "WEKO"}]}]}, "item_1_date_granted_11": {"attribute_name": "学位授与年月日", "attribute_value_mlt": [{"subitem_dategranted": "1999-03-24"}]}, "item_1_degree_grantor_5": {"attribute_name": "学位授与機関", "attribute_value_mlt": [{"subitem_degreegrantor": [{"subitem_degreegrantor_name": "総合研究大学院大学"}]}]}, "item_1_degree_name_6": {"attribute_name": "学位名", "attribute_value_mlt": [{"subitem_degreename": "博士（理学）"}]}, "item_1_description_1": {"attribute_name": "ID", "attribute_value_mlt": [{"subitem_description": "1999807", "subitem_description_type": "Other"}]}, "item_1_description_12": {"attribute_name": "要旨", "attribute_value_mlt": [{"subitem_description": "     Selection of a σ subunit of RNA polymerase is a powerful mechanism for switching transcription of a group of genes. For understanding the regulation of σ subunit utilization in Escherichia coli, I determined the intracellular levels of four members among 8 known σ subunits of E. coli strains, W3110 and MC4100, at various growth phases by a quantitative Western immunoblot analysis. The level of σ\u003cSUP\u003e70\u003c/SUP\u003e is rather constant throughout the growth, and those of σ\u003cSUP\u003e54\u003c/SUP\u003e and σ\u003cSUP\u003e28\u003c/SUP\u003e are maintained at 10 and 50% the level of σ\u003cSUP\u003e70\u003c/SUP\u003e in both strains growing at exponential and stationary phases, respectively. At the exponential growth phase σ\u003cSUP\u003e38\u003c/SUP\u003e is undetectable but increases to up to 30% of σ\u003cSUP\u003e70\u003c/SUP\u003e at the stationary phase, supporting the concept that σ\u003cSUP\u003e38\u003c/SUP\u003e plays a key role in transcription of the stationary phase - expressed genes. Stress - coupled change in the intracellular level was observed for two σ subunits: an increase in σ\u003cSUP\u003e38\u003c/SUP\u003e level and a decrease in σ\u003cSUP\u003e28\u003c/SUP\u003e level upon exposure to heat shock at the exponential phase; and the increase in σ\u003cSUP\u003e38\u003c/SUP\u003e level under a high osmolality condition at both the exponential and the stationary phases. I also found that the composition of σ subunits is different between bacterial strains, even under the same strain name. For instance, the compositions of σ\u003cSUP\u003e28\u003c/SUP\u003e and σ\u003cSUP\u003e38\u003c/SUP\u003e differ among three W3110 strains.\u003cbr /\u003e     The growth transition from exponential to stationary phase is accompanied by the replacement of RNA polymerase - associated σ\u003cSUP\u003e70\u003c/SUP\u003e subunit with σ\u003cSUP\u003e38\u003c/SUP\u003e. She found that the GST - fused σ\u003cSUP\u003e70\u003c/SUP\u003e in stationary - phase cell extracts exists as a complex with a new protein, and it was designated Rsd (Regulator of Sigma D). A gene located at 90 min on the E. coli chromosome encodes Rsd, and is transcribed from upstream and downstream promoters with σ\u003cSUP\u003e38\u003c/SUP\u003e and σ\u003cSUP\u003e70\u003c/SUP\u003e holoenzymes, respectively. Over - expressed and purified Rsd protein can form a complex with σ70 in vitro but not with any other σ subunits; σ\u003cSUP\u003e54\u003c/SUP\u003e, σ\u003cSUP\u003e38\u003c/SUP\u003e, σ\u003cSUP\u003e32\u003c/SUP\u003e, σ\u003cSUP\u003e28\u003c/SUP\u003e and σ\u003cSUP\u003e24\u003c/SUP\u003e. A binding assay with proteolytic fragments of σ\u003cSUP\u003e70\u003c/SUP\u003e suggested that Rsd interacts with the conserved region 4, the promoter  - 35 recognition domain, and its downstream of σ\u003cSUP\u003e70\u003c/SUP\u003e. The isolated Rsd inhibited transcription in vitro to various extents depending on the promoters used. The intracellular level of Rsd starts to increase during the transition from growing to stationary phase.\u003cbr /\u003e     Experiments using strains with disrupted rsd gene and those with overproduced Rsd indicated that Rsd is a negative factor for transcription directed by σ\u003cSUP\u003e70\u003c/SUP\u003e and a positive factor for that by σ\u003cSUP\u003e38\u003c/SUP\u003e. The results indicate that Rsd is a stationary - phase E. coli protein regulating the activity of the σ\u003cSUP\u003e70\u003c/SUP\u003e function, providing an evidence for the existence of the control of the activities as well as the levels of the σ subunits of RNA polymerase, contributing to a group switching of genes ofE. coli. Taking all the observations together, she discussed possible models for the switching mechanism.", "subitem_description_type": "Other"}]}, "item_1_description_18": {"attribute_name": "フォーマット", "attribute_value_mlt": [{"subitem_description": "application/pdf", "subitem_description_type": "Other"}]}, "item_1_description_7": {"attribute_name": "学位記番号", "attribute_value_mlt": [{"subitem_description": "総研大乙第64号", "subitem_description_type": "Other"}]}, "item_1_select_14": {"attribute_name": "所蔵", "attribute_value_mlt": [{"subitem_select_item": "有"}]}, "item_1_select_8": {"attribute_name": "研究科", "attribute_value_mlt": [{"subitem_select_item": "数物科学研究科"}]}, "item_1_select_9": {"attribute_name": "専攻", "attribute_value_mlt": [{"subitem_select_item": "18 遺伝学専攻"}]}, "item_1_text_10": {"attribute_name": "学位授与年度", "attribute_value_mlt": [{"subitem_text_value": "1998"}]}, "item_1_text_20": {"attribute_name": "業務メモ", "attribute_value_mlt": [{"subitem_text_value": "（2018年2月19日）本籍など個人情報の記載がある旧要旨・審査要旨を個人情報のない新しいものに差し替えた。承諾書等未確認。要確認該当項目修正のこと。"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "JISHAGE, Miki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "9782", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-02-17"}], "displaytype": "simple", "download_preview_message": "", "file_order": 0, "filename": "乙64_要旨.pdf", "filesize": [{"value": "259.9 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 259899.99999999997, "url": {"label": "要旨・審査要旨 / Abstract, Screening Result", "url": "https://ir.soken.ac.jp/record/946/files/乙64_要旨.pdf"}, "version_id": "6182e52b-a5ac-49ab-ade3-e3d6b6bf0627"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-02-17"}], "displaytype": "simple", "download_preview_message": "", "file_order": 1, "filename": "乙64_本文.pdf", "filesize": [{"value": "3.6 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 3600000.0, "url": {"label": "本文", "url": "https://ir.soken.ac.jp/record/946/files/乙64_本文.pdf"}, "version_id": "6fb5599e-48ca-47f8-9114-262ab473481d"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "thesis", "resourceuri": "http://purl.org/coar/resource_type/c_46ec"}]}, "item_title": "Control of the Synthesis and Functions of RNAPolymerase Sigma Subunits in Escherichia coli", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Control of the Synthesis and Functions of RNAPolymerase Sigma Subunits in Escherichia coli"}, {"subitem_title": "Control of the Synthesis and Functions of RNAPolymerase Sigma Subunits in Escherichia coli", "subitem_title_language": "en"}]}, "item_type_id": "1", "owner": "1", "path": ["20"], "permalink_uri": "https://ir.soken.ac.jp/records/946", "pubdate": {"attribute_name": "公開日", "attribute_value": "2010-02-22"}, "publish_date": "2010-02-22", "publish_status": "0", "recid": "946", "relation": {}, "relation_version_is_last": true, "title": ["Control of the Synthesis and Functions of RNAPolymerase Sigma Subunits in Escherichia coli"], "weko_shared_id": 1}