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Comprehensive characterization of differentially methylated regions associated with mouse imprinted genes
https://ir.soken.ac.jp/records/1020
https://ir.soken.ac.jp/records/1020d9f3e7ef-de59-494b-a0cd-9bc0fe34d46f
| 名前 / ファイル | ライセンス | アクション |
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要旨・審査要旨 (207.7 kB)
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本文 (1.6 MB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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| 公開日 | 2010-02-22 | |||||
| タイトル | ||||||
| タイトル | Comprehensive characterization of differentially methylated regions associated with mouse imprinted genes | |||||
| タイトル | ||||||
| タイトル | Comprehensive characterization of differentially methylated regions associated with mouse imprinted genes | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_46ec | |||||
| 資源タイプ | thesis | |||||
| 著者名 |
小林, 久人
× 小林, 久人 |
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| フリガナ |
コバヤシ, ヒサト
× コバヤシ, ヒサト |
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| 著者 |
KOBAYASHI, Hisato
× KOBAYASHI, Hisato |
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| 学位授与機関 | ||||||
| 学位授与機関名 | 総合研究大学院大学 | |||||
| 学位名 | ||||||
| 学位名 | 博士（理学） | |||||
| 学位記番号 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 総研大甲第957号 | |||||
| 研究科 | ||||||
| 値 | 生命科学研究科 | |||||
| 専攻 | ||||||
| 値 | 18 遺伝学専攻 | |||||
| 学位授与年月日 | ||||||
| 学位授与年月日 | 2006-03-24 | |||||
| 学位授与年度 | ||||||
| 値 | 2005 | |||||
| 要旨 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | Imprinted genes in mammals show monoallelic expression dependent<br /> on parental origin and are often associated with differentially methylated regions<br /> (DMRs). There are two classes of DMR: germline DMRs acquire gamete-specific<br /> methylation in either spermatogenesis or oogenesis and maintain the allelic<br /> methylation differences throughout development; secondary DMRs establish<br /> differential methylation patterns after fertilization. Targeted disruption of some<br /> germline DMRs showed that they dictate the allelic expression of nearby<br /> imprinted genes and the establishment of the allelic methylation of secondary<br /> DMRs. However, how the imprinting machinery recognizes germline DMRs is<br /> unknown. As a step toward elucidating the sequence features of the germline<br /> DMRs, I have determined the extents and boundaries of 15 germline<br /> mouse DMRs (including 12 maternally methylated and 3 paternally methylated ones) in<br /> 12.5-dpc embryos and sperm by bisulfite sequencing. I found that the average<br /> size of the DMRs was 2.7 kb and that their average G+C content was 54.2%.<br /> Oligonucleotide content analysis of the DMR sequences revealed that, although<br /> they are generally CpG rich, the paternally methylated DMRs contain less CpGs<br /> than the maternally methylated ones. Furthermore, based on the SOM analysis,<br /> I found that most germline DMRs have features distinct from typical mouse<br /> sequences. My findings provide a basis for the further characterization of DMRs.<br /> | |||||
| 所蔵 | ||||||
| 値 | 有 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
