Item type |
学位論文 / Thesis or Dissertation(1) |
公開日 |
2010-02-22 |
タイトル |
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タイトル |
Comprehensive characterization of differentially methylated regions associated with mouse imprinted genes |
タイトル |
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言語 |
en |
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タイトル |
Comprehensive characterization of differentially methylated regions associated with mouse imprinted genes |
言語 |
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言語 |
eng |
資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_46ec |
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資源タイプ |
thesis |
著者名 |
小林, 久人
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フリガナ |
コバヤシ, ヒサト
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著者 |
KOBAYASHI, Hisato
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学位授与機関 |
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学位授与機関名 |
総合研究大学院大学 |
学位名 |
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学位名 |
博士(理学) |
学位記番号 |
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内容記述タイプ |
Other |
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内容記述 |
総研大甲第957号 |
研究科 |
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値 |
生命科学研究科 |
専攻 |
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値 |
18 遺伝学専攻 |
学位授与年月日 |
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学位授与年月日 |
2006-03-24 |
学位授与年度 |
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2005 |
要旨 |
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内容記述タイプ |
Other |
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内容記述 |
Imprinted genes in mammals show monoallelic expression dependent<br /> on parental origin and are often associated with differentially methylated regions<br /> (DMRs). There are two classes of DMR: germline DMRs acquire gamete-specific<br /> methylation in either spermatogenesis or oogenesis and maintain the allelic<br /> methylation differences throughout development; secondary DMRs establish<br /> differential methylation patterns after fertilization. Targeted disruption of some<br /> germline DMRs showed that they dictate the allelic expression of nearby<br /> imprinted genes and the establishment of the allelic methylation of secondary<br /> DMRs. However, how the imprinting machinery recognizes germline DMRs is<br /> unknown. As a step toward elucidating the sequence features of the germline<br /> DMRs, I have determined the extents and boundaries of 15 germline<br /> mouse DMRs (including 12 maternally methylated and 3 paternally methylated ones) in<br /> 12.5-dpc embryos and sperm by bisulfite sequencing. I found that the average<br /> size of the DMRs was 2.7 kb and that their average G+C content was 54.2%.<br /> Oligonucleotide content analysis of the DMR sequences revealed that, although<br /> they are generally CpG rich, the paternally methylated DMRs contain less CpGs<br /> than the maternally methylated ones. Furthermore, based on the SOM analysis,<br /> I found that most germline DMRs have features distinct from typical mouse<br /> sequences. My findings provide a basis for the further characterization of DMRs.<br /> |
所蔵 |
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値 |
有 |
フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |