{"_buckets": {"deposit": "1c895ca4-7700-448e-8a64-c3408a1f7d9d"}, "_deposit": {"created_by": 1, "id": "1020", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "1020"}, "status": "published"}, "_oai": {"id": "oai:ir.soken.ac.jp:00001020", "sets": ["20"]}, "author_link": ["0", "0", "0"], "item_1_biblio_info_21": {"attribute_name": "書誌情報（ソート用）", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2006-03-24", "bibliographicIssueDateType": "Issued"}, "bibliographic_titles": [{}]}]}, "item_1_creator_2": {"attribute_name": "著者名", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "小林, 久人"}], "nameIdentifiers": [{"nameIdentifier": "0", "nameIdentifierScheme": "WEKO"}]}]}, "item_1_creator_3": {"attribute_name": "フリガナ", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "コバヤシ, ヒサト"}], "nameIdentifiers": [{"nameIdentifier": "0", "nameIdentifierScheme": "WEKO"}]}]}, "item_1_date_granted_11": {"attribute_name": "学位授与年月日", "attribute_value_mlt": [{"subitem_dategranted": "2006-03-24"}]}, "item_1_degree_grantor_5": {"attribute_name": "学位授与機関", "attribute_value_mlt": [{"subitem_degreegrantor": [{"subitem_degreegrantor_name": "総合研究大学院大学"}]}]}, "item_1_degree_name_6": {"attribute_name": "学位名", "attribute_value_mlt": [{"subitem_degreename": "博士（理学）"}]}, "item_1_description_1": {"attribute_name": "ID", "attribute_value_mlt": [{"subitem_description": "2006044", "subitem_description_type": "Other"}]}, "item_1_description_12": {"attribute_name": "要旨", "attribute_value_mlt": [{"subitem_description": "　　　Imprinted genes in mammals show monoallelic expression dependent\u003cbr /\u003e on parental origin and are often associated with differentially methylated regions\u003cbr /\u003e (DMRs). There are two classes of DMR: germline DMRs acquire gamete-specific\u003cbr /\u003e methylation in either spermatogenesis or oogenesis and maintain the allelic\u003cbr /\u003e methylation differences throughout development; secondary DMRs establish\u003cbr /\u003e differential methylation patterns after fertilization. Targeted disruption of some\u003cbr /\u003e germline DMRs showed that they dictate the allelic expression of nearby\u003cbr /\u003e imprinted genes and the establishment of the allelic methylation of secondary\u003cbr /\u003e DMRs. However, how the imprinting machinery recognizes germline DMRs is\u003cbr /\u003e unknown. As a step toward elucidating the sequence features of the germline\u003cbr /\u003e DMRs, I have determined the extents and boundaries of 15 germline\u003cbr /\u003e mouse DMRs (including 12 maternally methylated and 3 paternally methylated ones) in\u003cbr /\u003e 12.5-dpc embryos and sperm by bisulfite sequencing. I found that the average\u003cbr /\u003e size of the DMRs was 2.7 kb and that their average G+C content was 54.2%.\u003cbr /\u003e Oligonucleotide content analysis of the DMR sequences revealed that, although\u003cbr /\u003e they are generally CpG rich, the paternally methylated DMRs contain less CpGs\u003cbr /\u003e than the maternally methylated ones. Furthermore, based on the SOM analysis,\u003cbr /\u003e I found that most germline DMRs have features distinct from typical mouse\u003cbr /\u003e sequences. My findings provide a basis for the further characterization of DMRs.\u003cbr /\u003e", "subitem_description_type": "Other"}]}, "item_1_description_18": {"attribute_name": "フォーマット", "attribute_value_mlt": [{"subitem_description": "application/pdf", "subitem_description_type": "Other"}]}, "item_1_description_7": {"attribute_name": "学位記番号", "attribute_value_mlt": [{"subitem_description": "総研大甲第957号", "subitem_description_type": "Other"}]}, "item_1_select_14": {"attribute_name": "所蔵", "attribute_value_mlt": [{"subitem_select_item": "有"}]}, "item_1_select_8": {"attribute_name": "研究科", "attribute_value_mlt": [{"subitem_select_item": "生命科学研究科"}]}, "item_1_select_9": {"attribute_name": "専攻", "attribute_value_mlt": [{"subitem_select_item": "18 遺伝学専攻"}]}, "item_1_text_10": {"attribute_name": "学位授与年度", "attribute_value_mlt": [{"subitem_text_value": "2005"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "KOBAYASHI, Hisato", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "0", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-02-17"}], "displaytype": "simple", "download_preview_message": "", "file_order": 0, "filename": "甲957_要旨.pdf", "filesize": [{"value": "207.7 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 207700.0, "url": {"label": "要旨・審査要旨", "url": "https://ir.soken.ac.jp/record/1020/files/甲957_要旨.pdf"}, "version_id": "838b718f-0e85-43dd-aaff-eb7d2b757571"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-02-17"}], "displaytype": "simple", "download_preview_message": "", "file_order": 1, "filename": "甲957_本文.pdf", "filesize": [{"value": "1.6 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 1600000.0, "url": {"label": "本文", "url": "https://ir.soken.ac.jp/record/1020/files/甲957_本文.pdf"}, "version_id": "06376216-447d-4779-a94e-7c5c39e492a4"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "thesis", "resourceuri": "http://purl.org/coar/resource_type/c_46ec"}]}, "item_title": "Comprehensive characterization of differentially methylated regions associated with mouse imprinted genes", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Comprehensive characterization of differentially methylated regions associated with mouse imprinted genes"}, {"subitem_title": "Comprehensive characterization of differentially methylated regions associated with mouse imprinted genes", "subitem_title_language": "en"}]}, "item_type_id": "1", "owner": "1", "path": ["20"], "permalink_uri": "https://ir.soken.ac.jp/records/1020", "pubdate": {"attribute_name": "公開日", "attribute_value": "2010-02-22"}, "publish_date": "2010-02-22", "publish_status": "0", "recid": "1020", "relation": {}, "relation_version_is_last": true, "title": ["Comprehensive characterization of differentially methylated regions associated with mouse imprinted genes"], "weko_shared_id": 1}