| Item type |
学位論文 / Thesis or Dissertation(1) |
| 公開日 |
2010-02-22 |
| タイトル |
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|
タイトル |
Action organizations within single dendritic spines in CA1 pyramidal neurons studies with two-photon photoactivation |
| タイトル |
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|
タイトル |
Action organizations within single dendritic spines in CA1 pyramidal neurons studies with two-photon photoactivation |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_46ec |
|
資源タイプ |
thesis |
| 著者名 |
本蔵, 直樹
|
| フリガナ |
ホンクラ, ナオキ
|
| 著者 |
HONKURA, Naoki
|
| 学位授与機関 |
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学位授与機関名 |
総合研究大学院大学 |
| 学位名 |
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学位名 |
博士（理学） |
| 学位記番号 |
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内容記述タイプ |
Other |
|
内容記述 |
総研大甲第970号 |
| 研究科 |
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|
値 |
生命科学研究科 |
| 専攻 |
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|
値 |
20 生理科学専攻 |
| 学位授与年月日 |
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学位授与年月日 |
2006-03-24 |
| 学位授与年度 |
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値 |
2005 |
| 要旨 |
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内容記述タイプ |
Other |
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内容記述 |
The major cytoskeleton of dendritic spines is filamentous actin (F-actin).<br />Organizations of F-actin within individual spines, however, have not been<br />elucidated. I have here investigated sub-spine actin organizations using<br />two-photon photoactivation of PA-GFP fused with β-actin in rat CA1 pyramidal<br />neurons in slice culture preparations. I found segregated and discontinuous<br /.>organizations of two pools of F-actin, dynamic and stable pools, which tuned<br />over with time constants of 1.2 min and 17 min, respectively. The stable F-actin<br />pool was localized at the base of spine head, often intruding into thick spine neck,<br />while the dynamic pool occupied the rest of spine head. Fractions of the stable<br />F-actin pool were greater in larger spines, therefore, the entire F-actin poor was<br />more stable in larger spines. I succeeded in visualizing a retrograde flow of<br />F-actin in the dynamic pool from the apex to the base of spine, and found that<br />both the speeds (0.2-1.2 μm/min) and lengths (0.2-0.7μm) of the F-actin flow<br />were greater in spines with larger head volumes. Moreover, spine heads<br />rapidly shrank when actin polymerization was blocked by latrunculin A,<br />suggesting that the rate of actin polymerization in each spine actively and<br />continuously determines the volume of spine head via the length of F-actin.<br />Thus, I have revealed the sub-spine organizations of actin filaments that play<br />key role in spine structures and diversity. |
| 所蔵 |
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|
値 |
有 |
| フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |
要旨・審査要旨 (325.9 kB)
