{"_buckets": {"deposit": "148832ac-897b-4b0d-9f78-50011e3ee2f2"}, "_deposit": {"created_by": 1, "id": "1172", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "1172"}, "status": "published"}, "_oai": {"id": "oai:ir.soken.ac.jp:00001172", "sets": ["22"]}, "author_link": ["0", "0", "0"], "item_1_biblio_info_21": {"attribute_name": "書誌情報（ソート用）", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2006-03-24", "bibliographicIssueDateType": "Issued"}, "bibliographic_titles": [{}]}]}, "item_1_creator_2": {"attribute_name": "著者名", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "本蔵, 直樹"}], "nameIdentifiers": [{"nameIdentifier": "0", "nameIdentifierScheme": "WEKO"}]}]}, "item_1_creator_3": {"attribute_name": "フリガナ", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "ホンクラ, ナオキ"}], "nameIdentifiers": [{"nameIdentifier": "0", "nameIdentifierScheme": "WEKO"}]}]}, 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in slice culture preparations.　I found segregated and discontinuous\u003cbr /.\u003eorganizations of two pools of F-actin, dynamic and stable pools, which tuned\u003cbr /\u003eover with time constants of 1.2 min and 17 min, respectively.　The stable F-actin\u003cbr /\u003epool was localized at the base of spine head, often intruding into thick spine neck,\u003cbr /\u003ewhile the dynamic pool occupied the rest of spine head.　Fractions of the stable\u003cbr /\u003eF-actin pool were greater in larger spines, therefore, the entire F-actin poor was\u003cbr /\u003emore stable in larger spines.　I succeeded in visualizing a retrograde flow of\u003cbr /\u003eF-actin in the dynamic pool from the apex to the base of spine, and found that\u003cbr /\u003eboth the speeds (0.2-1.2 μm/min) and lengths (0.2-0.7μm) of the F-actin flow\u003cbr /\u003ewere greater in spines with larger head volumes.　Moreover, spine heads\u003cbr /\u003erapidly shrank when actin polymerization was blocked by latrunculin A,\u003cbr /\u003esuggesting that the rate of actin polymerization in each spine actively and\u003cbr /\u003econtinuously determines the volume of spine head via the length of F-actin.\u003cbr /\u003eThus, I have revealed the sub-spine organizations of actin filaments that play\u003cbr /\u003ekey role in spine structures and diversity.", "subitem_description_type": "Other"}]}, "item_1_description_18": {"attribute_name": "フォーマット", "attribute_value_mlt": [{"subitem_description": "application/pdf", "subitem_description_type": "Other"}]}, "item_1_description_7": {"attribute_name": "学位記番号", "attribute_value_mlt": [{"subitem_description": "総研大甲第970号", "subitem_description_type": "Other"}]}, "item_1_select_14": {"attribute_name": "所蔵", "attribute_value_mlt": [{"subitem_select_item": "有"}]}, "item_1_select_8": {"attribute_name": "研究科", "attribute_value_mlt": [{"subitem_select_item": "生命科学研究科"}]}, "item_1_select_9": {"attribute_name": "専攻", "attribute_value_mlt": [{"subitem_select_item": "20 生理科学専攻"}]}, "item_1_text_10": {"attribute_name": "学位授与年度", "attribute_value_mlt": [{"subitem_text_value": "2005"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "HONKURA, Naoki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "0", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-02-17"}], "displaytype": "simple", "download_preview_message": "", "file_order": 0, "filename": "甲970_要旨.pdf", "filesize": [{"value": "325.9 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 325900.0, "url": {"label": "要旨・審査要旨", "url": "https://ir.soken.ac.jp/record/1172/files/甲970_要旨.pdf"}, "version_id": "c0ec0d2f-b1ab-4a1c-85f7-46d3d3731b9d"}, 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[{"subitem_title": "Action organizations within single dendritic spines in CA1 pyramidal neurons studies with two-photon photoactivation"}, {"subitem_title": "Action organizations within single dendritic spines in CA1 pyramidal neurons studies with two-photon photoactivation", "subitem_title_language": "en"}]}, "item_type_id": "1", "owner": "1", "path": ["22"], "permalink_uri": "https://ir.soken.ac.jp/records/1172", "pubdate": {"attribute_name": "公開日", "attribute_value": "2010-02-22"}, "publish_date": "2010-02-22", "publish_status": "0", "recid": "1172", "relation": {}, "relation_version_is_last": true, "title": ["Action organizations within single dendritic spines in CA1 pyramidal neurons studies with two-photon photoactivation"], "weko_shared_id": -1}