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Action organizations within single dendritic spines in CA1 pyramidal neurons studies with two-photon photoactivation
https://ir.soken.ac.jp/records/1172
https://ir.soken.ac.jp/records/1172eb4d4ccd-864f-4900-9e1f-43966f5b280a
| 名前 / ファイル | ライセンス | アクション |
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要旨・審査要旨 (325.9 kB)
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本文 (6.8 MB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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| 公開日 | 2010-02-22 | |||||
| タイトル | ||||||
| タイトル | Action organizations within single dendritic spines in CA1 pyramidal neurons studies with two-photon photoactivation | |||||
| タイトル | ||||||
| タイトル | Action organizations within single dendritic spines in CA1 pyramidal neurons studies with two-photon photoactivation | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_46ec | |||||
| 資源タイプ | thesis | |||||
| 著者名 |
本蔵, 直樹
× 本蔵, 直樹 |
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| フリガナ |
ホンクラ, ナオキ
× ホンクラ, ナオキ |
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| 著者 |
HONKURA, Naoki
× HONKURA, Naoki |
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| 学位授与機関 | ||||||
| 学位授与機関名 | 総合研究大学院大学 | |||||
| 学位名 | ||||||
| 学位名 | 博士（理学） | |||||
| 学位記番号 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 総研大甲第970号 | |||||
| 研究科 | ||||||
| 値 | 生命科学研究科 | |||||
| 専攻 | ||||||
| 値 | 20 生理科学専攻 | |||||
| 学位授与年月日 | ||||||
| 学位授与年月日 | 2006-03-24 | |||||
| 学位授与年度 | ||||||
| 値 | 2005 | |||||
| 要旨 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | The major cytoskeleton of dendritic spines is filamentous actin (F-actin).<br />Organizations of F-actin within individual spines, however, have not been<br />elucidated. I have here investigated sub-spine actin organizations using<br />two-photon photoactivation of PA-GFP fused with β-actin in rat CA1 pyramidal<br />neurons in slice culture preparations. I found segregated and discontinuous<br /.>organizations of two pools of F-actin, dynamic and stable pools, which tuned<br />over with time constants of 1.2 min and 17 min, respectively. The stable F-actin<br />pool was localized at the base of spine head, often intruding into thick spine neck,<br />while the dynamic pool occupied the rest of spine head. Fractions of the stable<br />F-actin pool were greater in larger spines, therefore, the entire F-actin poor was<br />more stable in larger spines. I succeeded in visualizing a retrograde flow of<br />F-actin in the dynamic pool from the apex to the base of spine, and found that<br />both the speeds (0.2-1.2 μm/min) and lengths (0.2-0.7μm) of the F-actin flow<br />were greater in spines with larger head volumes. Moreover, spine heads<br />rapidly shrank when actin polymerization was blocked by latrunculin A,<br />suggesting that the rate of actin polymerization in each spine actively and<br />continuously determines the volume of spine head via the length of F-actin.<br />Thus, I have revealed the sub-spine organizations of actin filaments that play<br />key role in spine structures and diversity. | |||||
| 所蔵 | ||||||
| 値 | 有 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
