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Nuclear localization of reporter genes activated by curved DNA
https://ir.soken.ac.jp/records/3956
https://ir.soken.ac.jp/records/3956c77cc25f-e617-404d-a521-234f7cc9e1e3
| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2013-09-18 | |||||
| タイトル | ||||||
| タイトル | Nuclear localization of reporter genes activated by curved DNA | |||||
| タイトル | ||||||
| タイトル | Nuclear localization of reporter genes activated by curved DNA | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| アクセス権 | ||||||
| アクセス権 | metadata only access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||
| 著者 |
UDAGAWA, Koji
× UDAGAWA, Koji× KIMURA, Hajime× TANABE, Hideyuki× OHYAMA, Takashi |
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| 著者別名 |
田辺, 秀之
× 田辺, 秀之 |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Curved DNA structures with a left-handed superhelical conformation can activate eukaryotic transcription. Mechanistically, these structures favor binding to histone cores and can function as a docking site for sliding nucleosomes. Thus, promoters with this kind of curved DNA can adopt a more open structure, facilitating transcription initiation. However, whether the curved DNA segment can affect localization of a reporter gene is an open question. Localization of a gene in the nucleus often plays an important role in its expression and this phenomenon may also have a curved DNA-dependent mechanism. We examined this issue in transient and stable assay systems using a 180-bp synthetic curved DNA with a left-handed superhelical conformation. The results clearly showed that curved DNA of this kind does not have a property to deliver reporter constructs to nuclear positions that are preferable for transcription. We also identify the spatial location to which electroporation delivers a reporter plasmid in the nucleus. | |||||
| 書誌情報 |
Journal of Bioscience and Bioengineering en : Journal of Bioscience and Bioengineering 巻 113, 号 4, p. 431-437, 発行日 2012-04 |
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| 出版者 | ||||||
| 出版者 | Elsevier | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1389-1723 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1016/j.jbiosc.2011.11.022 | |||||
| 関連名称 | 10.1016/j.jbiosc.2011.11.022 | |||||
| 権利 | ||||||
| 権利情報 | © 2011 The Society for Biotechnology, Japan. | |||||
