| Item type |
学術雑誌論文 / Journal Article(1) |
| 公開日 |
2013-09-18 |
| タイトル |
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|
タイトル |
Nuclear localization of reporter genes activated by curved DNA |
| タイトル |
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タイトル |
Nuclear localization of reporter genes activated by curved DNA |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_6501 |
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資源タイプ |
journal article |
| アクセス権 |
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アクセス権 |
metadata only access |
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アクセス権URI |
http://purl.org/coar/access_right/c_14cb |
| 著者 |
UDAGAWA, Koji
KIMURA, Hajime
TANABE, Hideyuki
OHYAMA, Takashi
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| 著者別名 |
田辺, 秀之
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| 抄録 |
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内容記述タイプ |
Abstract |
|
内容記述 |
Curved DNA structures with a left-handed superhelical conformation can activate eukaryotic transcription. Mechanistically, these structures favor binding to histone cores and can function as a docking site for sliding nucleosomes. Thus, promoters with this kind of curved DNA can adopt a more open structure, facilitating transcription initiation. However, whether the curved DNA segment can affect localization of a reporter gene is an open question. Localization of a gene in the nucleus often plays an important role in its expression and this phenomenon may also have a curved DNA-dependent mechanism. We examined this issue in transient and stable assay systems using a 180-bp synthetic curved DNA with a left-handed superhelical conformation. The results clearly showed that curved DNA of this kind does not have a property to deliver reporter constructs to nuclear positions that are preferable for transcription. We also identify the spatial location to which electroporation delivers a reporter plasmid in the nucleus. |
| 書誌情報 |
Journal of Bioscience and Bioengineering
en : Journal of Bioscience and Bioengineering
巻 113,
号 4,
p. 431-437,
発行日 2012-04
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| 出版者 |
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出版者 |
Elsevier |
| ISSN |
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収録物識別子タイプ |
ISSN |
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収録物識別子 |
1389-1723 |
| DOI |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1016/j.jbiosc.2011.11.022 |
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関連名称 |
10.1016/j.jbiosc.2011.11.022 |
| 権利 |
|
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権利情報 |
© 2011 The Society for Biotechnology, Japan. |
