{"_buckets": {"deposit": "e14854aa-0257-4c67-8b56-f270c914bd6c"}, "_deposit": {"created_by": 21, "id": "3957", "owners": [21], "pid": {"revision_id": 0, "type": "depid", "value": "3957"}, "status": "published"}, "_oai": {"id": "oai:ir.soken.ac.jp:00003957", "sets": ["293"]}, "author_link": ["2064", "2065", "368"], "item_10001_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2011-09", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "16", "bibliographicPageStart": "e106", "bibliographicVolumeNumber": "39", "bibliographic_titles": [{"bibliographic_title": "Nucleic Acids Research"}, {"bibliographic_title": "Nucleic Acids Research", "bibliographic_titleLang": "en"}]}]}, "item_10001_description_19": {"attribute_name": "フォーマット", "attribute_value_mlt": [{"subitem_description": "application/pdf", "subitem_description_type": "Other"}]}, "item_10001_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Gene amplification contributes to a variety of biological phenomena, including malignant progression and drug resistance. However, details of the molecular mechanisms remain to be determined. Here, we have developed a gene amplification system in yeast and mammalian cells that is based on double rolling-circle replication (DRCR). Cre-lox system is used to efficiently induce DRCR utilizing a recombinational process coupled with replication. This system shows distinctive features seen in amplification of oncogenes and drug-resistance genes: (i) intra- and extrachromosomal amplification, (ii) intensive chromosome rearrangement and (iii) scattered-type amplification resembling those seen in cancer cells. This system can serve as a model for amplification of oncogenes and drug-resistance genes, and improve amplification systems used for making pharmaceutical proteins in mammalian cells. ", "subitem_description_type": "Abstract"}]}, "item_10001_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Oxford University Press"}]}, "item_10001_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1093/nar/gkr442"}], "subitem_relation_type": "isIdenticalTo", "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1093/nar/gkr442", "subitem_relation_type_select": "DOI"}}]}, "item_10001_relation_17": {"attribute_name": "関連サイト", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "Copyright"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "http://www.oxfordjournals.org/access_purchase/self-archiving_policyc.html", "subitem_relation_type_select": "URI"}}]}, "item_10001_rights_15": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "© The Author(s) 2011"}]}, "item_10001_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0305-1048", "subitem_source_identifier_type": "ISSN"}]}, "item_10001_version_type_20": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "WATANABE, Takaaki "}], "nameIdentifiers": [{"nameIdentifier": "2064", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "TANABE, Hideyuki"}], "nameIdentifiers": [{"nameIdentifier": "368", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "HORIUCHI, Takashi"}], "nameIdentifiers": [{"nameIdentifier": "2065", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2013-09-18"}], "displaytype": "simple", "download_preview_message": "", "file_order": 0, "filename": "e106.full.pdf", "filesize": [{"value": "6.4 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 6400000.0, "url": {"label": "e106.full", "url": "https://ir.soken.ac.jp/record/3957/files/e106.full.pdf"}, "version_id": "a4dbe9ef-8299-4a24-a9a6-c7aaef56d212"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Gene amplification system based on double rolling-circle replication as a model for oncogene-type amplification", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Gene amplification system based on double rolling-circle replication as a model for oncogene-type amplification"}, {"subitem_title": "Gene amplification system based on double rolling-circle replication as a model for oncogene-type amplification", "subitem_title_language": "en"}]}, "item_type_id": "10001", "owner": "21", "path": ["112", "293"], "permalink_uri": "https://ir.soken.ac.jp/records/3957", "pubdate": {"attribute_name": "公開日", "attribute_value": "2013-09-18"}, "publish_date": "2013-09-18", "publish_status": "0", "recid": "3957", "relation": {}, "relation_version_is_last": true, "title": ["Gene amplification system based on double rolling-circle replication as a model for oncogene-type amplification"], "weko_shared_id": 21}