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Gene amplification system based on double rolling-circle replication as a model for oncogene-type amplification
https://ir.soken.ac.jp/records/3957
https://ir.soken.ac.jp/records/3957927cbaaf-16fe-41df-90da-021fc929651c
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
e106.full (6.4 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||
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| 公開日 | 2013-09-18 | |||||||||||
| タイトル | ||||||||||||
| タイトル | Gene amplification system based on double rolling-circle replication as a model for oncogene-type amplification | |||||||||||
| タイトル | ||||||||||||
| タイトル | Gene amplification system based on double rolling-circle replication as a model for oncogene-type amplification | |||||||||||
| 言語 | en | |||||||||||
| 言語 | ||||||||||||
| 言語 | eng | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
| 資源タイプ | journal article | |||||||||||
| 著者 |
WATANABE, Takaaki
× WATANABE, Takaaki
× TANABE, Hideyuki
× HORIUCHI, Takashi
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| 抄録 | ||||||||||||
| 内容記述タイプ | Abstract | |||||||||||
| 内容記述 | Gene amplification contributes to a variety of biological phenomena, including malignant progression and drug resistance. However, details of the molecular mechanisms remain to be determined. Here, we have developed a gene amplification system in yeast and mammalian cells that is based on double rolling-circle replication (DRCR). Cre-lox system is used to efficiently induce DRCR utilizing a recombinational process coupled with replication. This system shows distinctive features seen in amplification of oncogenes and drug-resistance genes: (i) intra- and extrachromosomal amplification, (ii) intensive chromosome rearrangement and (iii) scattered-type amplification resembling those seen in cancer cells. This system can serve as a model for amplification of oncogenes and drug-resistance genes, and improve amplification systems used for making pharmaceutical proteins in mammalian cells. | |||||||||||
| 書誌情報 |
Nucleic Acids Research en : Nucleic Acids Research 巻 39, 号 16, p. e106, 発行日 2011-09 |
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| 出版者 | ||||||||||||
| 出版者 | Oxford University Press | |||||||||||
| ISSN | ||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||
| 収録物識別子 | 0305-1048 | |||||||||||
| DOI | ||||||||||||
| 関連タイプ | isIdenticalTo | |||||||||||
| 識別子タイプ | DOI | |||||||||||
| 関連識別子 | https://doi.org/10.1093/nar/gkr442 | |||||||||||
| 関連名称 | 10.1093/nar/gkr442 | |||||||||||
| 権利 | ||||||||||||
| 権利情報 | © The Author(s) 2011 | |||||||||||
| 関連サイト | ||||||||||||
| 識別子タイプ | URI | |||||||||||
| 関連識別子 | http://www.oxfordjournals.org/access_purchase/self-archiving_policyc.html | |||||||||||
| 関連名称 | Copyright | |||||||||||
| フォーマット | ||||||||||||
| 内容記述タイプ | Other | |||||||||||
| 内容記述 | application/pdf | |||||||||||
| 著者版フラグ | ||||||||||||
| 出版タイプ | VoR | |||||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||||
