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Gene amplification system based on double rolling-circle replication as a model for oncogene-type amplification
https://ir.soken.ac.jp/records/3957
https://ir.soken.ac.jp/records/3957927cbaaf-16fe-41df-90da-021fc929651c
名前 / ファイル | ライセンス | アクション |
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2013-09-18 | |||||
タイトル | ||||||
タイトル | Gene amplification system based on double rolling-circle replication as a model for oncogene-type amplification | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Gene amplification system based on double rolling-circle replication as a model for oncogene-type amplification | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
WATANABE, Takaaki
× WATANABE, Takaaki× TANABE, Hideyuki× HORIUCHI, Takashi |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Gene amplification contributes to a variety of biological phenomena, including malignant progression and drug resistance. However, details of the molecular mechanisms remain to be determined. Here, we have developed a gene amplification system in yeast and mammalian cells that is based on double rolling-circle replication (DRCR). Cre-lox system is used to efficiently induce DRCR utilizing a recombinational process coupled with replication. This system shows distinctive features seen in amplification of oncogenes and drug-resistance genes: (i) intra- and extrachromosomal amplification, (ii) intensive chromosome rearrangement and (iii) scattered-type amplification resembling those seen in cancer cells. This system can serve as a model for amplification of oncogenes and drug-resistance genes, and improve amplification systems used for making pharmaceutical proteins in mammalian cells. | |||||
書誌情報 |
Nucleic Acids Research en : Nucleic Acids Research 巻 39, 号 16, p. e106, 発行日 2011-09 |
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出版者 | ||||||
出版者 | Oxford University Press | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0305-1048 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1093/nar/gkr442 | |||||
関連名称 | 10.1093/nar/gkr442 | |||||
権利 | ||||||
権利情報 | © The Author(s) 2011 | |||||
関連サイト | ||||||
識別子タイプ | URI | |||||
関連識別子 | http://www.oxfordjournals.org/access_purchase/self-archiving_policyc.html | |||||
関連名称 | Copyright | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |