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  1. 010 学術雑誌論文
  2. 宅野, 将平 / TAKUNO, Shohei

Simple and efficient methods for S genotyping and S screening in genus Brassica by dot-blot analysis

https://ir.soken.ac.jp/records/4612
https://ir.soken.ac.jp/records/4612
3850c648-80d1-4f73-99ab-8356a4fe5973
Item type 学術雑誌論文 / Journal Article(1)
公開日 2014-06-05
タイトル
タイトル Simple and efficient methods for S genotyping and S screening in genus Brassica by dot-blot analysis
タイトル
タイトル Simple and efficient methods for S genotyping and S screening in genus Brassica by dot-blot analysis
言語 en
言語
言語 eng
資源タイプ
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
資源タイプ journal article
アクセス権
アクセス権 metadata only access
アクセス権URI http://purl.org/coar/access_right/c_14cb
著者 OIKAWA, Eriko

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OIKAWA, Eriko

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TAKUNO, Shohei

× TAKUNO, Shohei

TAKUNO, Shohei

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et, al.

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et, al.

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著者別名 宅野, 将平

× 宅野, 将平

宅野, 将平

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抄録
内容記述タイプ Abstract
内容記述 In F1 hybrid breeding of Brassica vegetables utilizing the self-incompatibility system, identification of S genotypes in breeding lines is required. In the present study, we developed S-tester lines of 87 S haplotypes, i.e., 42 S haplotypes in B. rapa and 45 S haplotypes in B. oleracea. With these materials, we established a simple, efficient, and reliable dot-blot technique for S genotyping for 40 S haplotypes of B. rapa and and 33 of B. oleracea using allele-specific oligonucleotide probes and allele-specific primer pairs designed from sequences of each SP11 allele. In this method, DNA fragments amplified using multiplex primer pairs with digoxigenin-dUTP were hybridized with dot-blotted allele-specific oligonucleotide probes with distinct signals. In addition, we developed a screening method for identification of plants harboring a particular S haplotype using a labeled allele-specific oligonucleotide probe. This method is considered to be useful for purity testing of F1 hybrid seeds.
書誌情報 Molecular Breeding
en : Molecular Breeding

巻 28, 号 1, p. 1-12, 発行日 2011-06
出版者
出版者 SpringerLink
ISSN
収録物識別子タイプ ISSN
収録物識別子 1380-3743
DOI
識別子タイプ DOI
関連識別子 https://doi.org/10.1007/s11032-010-9455-8
関連名称 10.1007/s11032-010-9455-8
権利
権利情報 SpringerLink(The original publication is available at www.springerlink.com)
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