{"_buckets": {"deposit": "1e225cf9-623e-4e5a-8648-bc7e99abeb76"}, "_deposit": {"created_by": 21, "id": "4612", "owners": [21], "pid": {"revision_id": 0, "type": "depid", "value": "4612"}, "status": "published"}, "_oai": {"id": "oai:ir.soken.ac.jp:00004612", "sets": ["340"]}, "author_link": ["768", "3119", "2962", "2937"], "item_10001_biblio_info_7": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2011-06", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "12", "bibliographicPageStart": "1", "bibliographicVolumeNumber": "28", "bibliographic_titles": [{"bibliographic_title": "Molecular Breeding "}, {"bibliographic_title": "Molecular Breeding ", "bibliographic_titleLang": "en"}]}]}, "item_10001_creator_3": {"attribute_name": "著者別名", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "宅野, 将平"}], "nameIdentifiers": [{"nameIdentifier": "2937", "nameIdentifierScheme": "WEKO"}]}]}, "item_10001_description_5": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "In F1 hybrid breeding of Brassica vegetables utilizing the self-incompatibility system, identification of S genotypes in breeding lines is required. In the present study, we developed S-tester lines of 87 S haplotypes, i.e., 42 S haplotypes in B. rapa and 45 S haplotypes in B. oleracea. With these materials, we established a simple, efficient, and reliable dot-blot technique for S genotyping for 40 S haplotypes of B. rapa and and 33 of B. oleracea using allele-specific oligonucleotide probes and allele-specific primer pairs designed from sequences of each SP11 allele. In this method, DNA fragments amplified using multiplex primer pairs with digoxigenin-dUTP were hybridized with dot-blotted allele-specific oligonucleotide probes with distinct signals. In addition, we developed a screening method for identification of plants harboring a particular S haplotype using a labeled allele-specific oligonucleotide probe. This method is considered to be useful for purity testing of F1 hybrid seeds.", "subitem_description_type": "Abstract"}]}, "item_10001_publisher_8": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "SpringerLink "}]}, "item_10001_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "10.1007/s11032-010-9455-8"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1007/s11032-010-9455-8", "subitem_relation_type_select": "DOI"}}]}, "item_10001_rights_15": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "SpringerLink(The original publication is available at www.springerlink.com) "}]}, "item_10001_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1380-3743", "subitem_source_identifier_type": "ISSN"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "metadata only access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_14cb"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "OIKAWA, Eriko"}], "nameIdentifiers": [{"nameIdentifier": "2962", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "TAKUNO, Shohei"}], "nameIdentifiers": [{"nameIdentifier": "768", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "et, al."}], "nameIdentifiers": [{"nameIdentifier": "3119", "nameIdentifierScheme": "WEKO"}]}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Simple and efficient methods for S genotyping and S screening in genus Brassica by dot-blot analysis", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Simple and efficient methods for S genotyping and S screening in genus Brassica by dot-blot analysis"}, {"subitem_title": "Simple and efficient methods for S genotyping and S screening in genus Brassica by dot-blot analysis", "subitem_title_language": "en"}]}, "item_type_id": "10001", "owner": "21", "path": ["339", "340"], "permalink_uri": "https://ir.soken.ac.jp/records/4612", "pubdate": {"attribute_name": "公開日", "attribute_value": "2014-06-05"}, "publish_date": "2014-06-05", "publish_status": "0", "recid": "4612", "relation": {}, "relation_version_is_last": true, "title": ["Simple and efficient methods for S genotyping and S screening in genus Brassica by dot-blot analysis"], "weko_shared_id": 21}