WEKO3
アイテム
Simple and efficient methods for S genotyping and S screening in genus Brassica by dot-blot analysis
https://ir.soken.ac.jp/records/4612
https://ir.soken.ac.jp/records/46123850c648-80d1-4f73-99ab-8356a4fe5973
| Item type | 学術雑誌論文 / Journal Article(1) | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2014-06-05 | |||||||||||
| タイトル | ||||||||||||
| タイトル | Simple and efficient methods for S genotyping and S screening in genus Brassica by dot-blot analysis | |||||||||||
| タイトル | ||||||||||||
| タイトル | Simple and efficient methods for S genotyping and S screening in genus Brassica by dot-blot analysis | |||||||||||
| 言語 | en | |||||||||||
| 言語 | ||||||||||||
| 言語 | eng | |||||||||||
| 資源タイプ | ||||||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
| 資源タイプ | journal article | |||||||||||
| アクセス権 | ||||||||||||
| アクセス権 | metadata only access | |||||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_14cb | |||||||||||
| 著者 |
OIKAWA, Eriko
× OIKAWA, Eriko
× TAKUNO, Shohei
× et, al.
|
|||||||||||
| 著者別名 |
宅野, 将平
× 宅野, 将平
|
|||||||||||
| 抄録 | ||||||||||||
| 内容記述タイプ | Abstract | |||||||||||
| 内容記述 | In F1 hybrid breeding of Brassica vegetables utilizing the self-incompatibility system, identification of S genotypes in breeding lines is required. In the present study, we developed S-tester lines of 87 S haplotypes, i.e., 42 S haplotypes in B. rapa and 45 S haplotypes in B. oleracea. With these materials, we established a simple, efficient, and reliable dot-blot technique for S genotyping for 40 S haplotypes of B. rapa and and 33 of B. oleracea using allele-specific oligonucleotide probes and allele-specific primer pairs designed from sequences of each SP11 allele. In this method, DNA fragments amplified using multiplex primer pairs with digoxigenin-dUTP were hybridized with dot-blotted allele-specific oligonucleotide probes with distinct signals. In addition, we developed a screening method for identification of plants harboring a particular S haplotype using a labeled allele-specific oligonucleotide probe. This method is considered to be useful for purity testing of F1 hybrid seeds. | |||||||||||
| 書誌情報 |
Molecular Breeding en : Molecular Breeding 巻 28, 号 1, p. 1-12, 発行日 2011-06 |
|||||||||||
| 出版者 | ||||||||||||
| 出版者 | SpringerLink | |||||||||||
| ISSN | ||||||||||||
| 収録物識別子タイプ | ISSN | |||||||||||
| 収録物識別子 | 1380-3743 | |||||||||||
| DOI | ||||||||||||
| 識別子タイプ | DOI | |||||||||||
| 関連識別子 | https://doi.org/10.1007/s11032-010-9455-8 | |||||||||||
| 関連名称 | 10.1007/s11032-010-9455-8 | |||||||||||
| 権利 | ||||||||||||
| 権利情報 | SpringerLink(The original publication is available at www.springerlink.com) | |||||||||||
