Item type |
学位論文 / Thesis or Dissertation(1) |
公開日 |
2010-02-22 |
タイトル |
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タイトル |
Sequence determination of region required for nuclear import and export of β-catenin: Implication for distinct molecular interactions involved in bi-directional nuclear pore passage. |
タイトル |
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言語 |
en |
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タイトル |
Sequence determination of region required for nuclear import and export of β-catenin: Implication for distinct molecular interactions involved in bi-directional nuclear pore passage. |
言語 |
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言語 |
jpn |
資源タイプ |
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資源タイプ識別子 |
http://purl.org/coar/resource_type/c_46ec |
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資源タイプ |
thesis |
著者名 |
小池, 牧子
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フリガナ |
コイケ, マキコ
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著者 |
KOIKE, Makiko
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学位授与機関 |
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学位授与機関名 |
総合研究大学院大学 |
学位名 |
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学位名 |
博士(理学) |
学位記番号 |
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内容記述タイプ |
Other |
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内容記述 |
総研大甲第723号 |
研究科 |
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値 |
生命科学研究科 |
専攻 |
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値 |
18 遺伝学専攻 |
学位授与年月日 |
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学位授与年月日 |
2003-09-30 |
学位授与年度 |
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2003 |
要旨 |
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内容記述タイプ |
Other |
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内容記述 |
The nucleocytoplasmic transport of macromolecules occurs through the nuclear pore complex (NPC). Most transport events through the NPC are mediated by transport receptor molecules. The localization of β-catenin to the nucleus is a crucial step in the transduction of the Wnt/Wingless signal. Upon activation of the Wnt pathway, β-catenin accumulates in the nucleus. Previous studies indicated that β-catenin could translocate on its own through the nuclear pores without the aid of small GTPase Ran, and shuttles between the cytoplasm and the nucleus. The export of β-catenin also occurs in a Ran-independent manner. Unlike receptor-mediated transport, β-catenin transport appears to use a rather unconventional mechanism, possibly through the direct interaction with NPC components. In this study, I have analyzed the sequence requirement of β-catenin for its Ran-independent import and export in living mammalian cells and in the in vitro transport assay using semi-intact cells. I confirmed that a β-catenin fragment containing both Armadillo repeats 10- 12 and the C-terminus possesses most strong nuclear import and export activity. Further dissection of this fragment showed that C-terminus of β-catenin is most important for its import and export. Moreover, I found that region required for β-catenin import and export overlaps, but they are not identical. Competition studies using different transport receptors under different conditions indicated that interaction(s) required for β-catenin import and export differ at NPC. |
所蔵 |
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値 |
有 |
フォーマット |
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内容記述タイプ |
Other |
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内容記述 |
application/pdf |