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Sequence determination of region required for nuclear import and export of β-catenin: Implication for distinct molecular interactions involved in bi-directional nuclear pore passage.
https://ir.soken.ac.jp/records/989
https://ir.soken.ac.jp/records/989d613d341-63bd-4948-aa99-f663de4875b1
| 名前 / ファイル | ライセンス | アクション |
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要旨・審査要旨 / Abstract, Screening Result (162.3 kB)
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本文 (2.9 MB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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| 公開日 | 2010-02-22 | |||||
| タイトル | ||||||
| タイトル | Sequence determination of region required for nuclear import and export of β-catenin: Implication for distinct molecular interactions involved in bi-directional nuclear pore passage. | |||||
| タイトル | ||||||
| タイトル | Sequence determination of region required for nuclear import and export of β-catenin: Implication for distinct molecular interactions involved in bi-directional nuclear pore passage. | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_46ec | |||||
| 資源タイプ | thesis | |||||
| 著者名 |
小池, 牧子
× 小池, 牧子 |
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| フリガナ |
コイケ, マキコ
× コイケ, マキコ |
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| 著者 |
KOIKE, Makiko
× KOIKE, Makiko |
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| 学位授与機関 | ||||||
| 学位授与機関名 | 総合研究大学院大学 | |||||
| 学位名 | ||||||
| 学位名 | 博士（理学） | |||||
| 学位記番号 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 総研大甲第723号 | |||||
| 研究科 | ||||||
| 値 | 生命科学研究科 | |||||
| 専攻 | ||||||
| 値 | 18 遺伝学専攻 | |||||
| 学位授与年月日 | ||||||
| 学位授与年月日 | 2003-09-30 | |||||
| 学位授与年度 | ||||||
| 値 | 2003 | |||||
| 要旨 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | The nucleocytoplasmic transport of macromolecules occurs through the nuclear pore complex (NPC). Most transport events through the NPC are mediated by transport receptor molecules. The localization of β-catenin to the nucleus is a crucial step in the transduction of the Wnt/Wingless signal. Upon activation of the Wnt pathway, β-catenin accumulates in the nucleus. Previous studies indicated that β-catenin could translocate on its own through the nuclear pores without the aid of small GTPase Ran, and shuttles between the cytoplasm and the nucleus. The export of β-catenin also occurs in a Ran-independent manner. Unlike receptor-mediated transport, β-catenin transport appears to use a rather unconventional mechanism, possibly through the direct interaction with NPC components. In this study, I have analyzed the sequence requirement of β-catenin for its Ran-independent import and export in living mammalian cells and in the in vitro transport assay using semi-intact cells. I confirmed that a β-catenin fragment containing both Armadillo repeats 10- 12 and the C-terminus possesses most strong nuclear import and export activity. Further dissection of this fragment showed that C-terminus of β-catenin is most important for its import and export. Moreover, I found that region required for β-catenin import and export overlaps, but they are not identical. Competition studies using different transport receptors under different conditions indicated that interaction(s) required for β-catenin import and export differ at NPC. | |||||
| 所蔵 | ||||||
| 値 | 有 | |||||
| フォーマット | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | application/pdf | |||||
