| Item type |
学位論文 / Thesis or Dissertation(1) |
| 公開日 |
2010-02-22 |
| タイトル |
|
|
タイトル |
Sequence determination of region required for nuclear import and export of β-catenin: Implication for distinct molecular interactions involved in bi-directional nuclear pore passage. |
| タイトル |
|
|
タイトル |
Sequence determination of region required for nuclear import and export of β-catenin: Implication for distinct molecular interactions involved in bi-directional nuclear pore passage. |
|
言語 |
en |
| 言語 |
|
|
言語 |
jpn |
| 資源タイプ |
|
|
資源タイプ識別子 |
http://purl.org/coar/resource_type/c_46ec |
|
資源タイプ |
thesis |
| 著者名 |
小池, 牧子
|
| フリガナ |
コイケ, マキコ
|
| 著者 |
KOIKE, Makiko
|
| 学位授与機関 |
|
|
|
学位授与機関名 |
総合研究大学院大学 |
| 学位名 |
|
|
学位名 |
博士（理学） |
| 学位記番号 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
総研大甲第723号 |
| 研究科 |
|
|
値 |
生命科学研究科 |
| 専攻 |
|
|
値 |
18 遺伝学専攻 |
| 学位授与年月日 |
|
|
学位授与年月日 |
2003-09-30 |
| 学位授与年度 |
|
|
値 |
2003 |
| 要旨 |
|
|
内容記述タイプ |
Other |
|
内容記述 |
The nucleocytoplasmic transport of macromolecules occurs through the nuclear pore complex (NPC). Most transport events through the NPC are mediated by transport receptor molecules. The localization of β-catenin to the nucleus is a crucial step in the transduction of the Wnt/Wingless signal. Upon activation of the Wnt pathway, β-catenin accumulates in the nucleus. Previous studies indicated that β-catenin could translocate on its own through the nuclear pores without the aid of small GTPase Ran, and shuttles between the cytoplasm and the nucleus. The export of β-catenin also occurs in a Ran-independent manner. Unlike receptor-mediated transport, β-catenin transport appears to use a rather unconventional mechanism, possibly through the direct interaction with NPC components. In this study, I have analyzed the sequence requirement of β-catenin for its Ran-independent import and export in living mammalian cells and in the in vitro transport assay using semi-intact cells. I confirmed that a β-catenin fragment containing both Armadillo repeats 10- 12 and the C-terminus possesses most strong nuclear import and export activity. Further dissection of this fragment showed that C-terminus of β-catenin is most important for its import and export. Moreover, I found that region required for β-catenin import and export overlaps, but they are not identical. Competition studies using different transport receptors under different conditions indicated that interaction(s) required for β-catenin import and export differ at NPC. |
| 所蔵 |
|
|
値 |
有 |
| フォーマット |
|
|
内容記述タイプ |
Other |
|
内容記述 |
application/pdf |
要旨・審査要旨 / Abstract, Screening Result (162.3 kB)
