{"_buckets": {"deposit": "06c7f6a6-d3fe-4968-bf01-ccae1c5821c7"}, "_deposit": {"created_by": 1, "id": "989", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "989"}, "status": "published"}, "_oai": {"id": "oai:ir.soken.ac.jp:00000989", "sets": ["20"]}, "author_link": ["10105", "10104", "10106"], "item_1_biblio_info_21": {"attribute_name": "書誌情報（ソート用）", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2003-09-30", "bibliographicIssueDateType": "Issued"}, "bibliographic_titles": [{}]}]}, "item_1_creator_2": {"attribute_name": "著者名", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "小池, 牧子"}], "nameIdentifiers": [{"nameIdentifier": "10104", "nameIdentifierScheme": "WEKO"}]}]}, "item_1_creator_3": {"attribute_name": "フリガナ", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "コイケ, マキコ"}], "nameIdentifiers": [{"nameIdentifier": "10105", "nameIdentifierScheme": "WEKO"}]}]}, "item_1_date_granted_11": {"attribute_name": "学位授与年月日", "attribute_value_mlt": [{"subitem_dategranted": "2003-09-30"}]}, "item_1_degree_grantor_5": {"attribute_name": "学位授与機関", "attribute_value_mlt": [{"subitem_degreegrantor": [{"subitem_degreegrantor_name": "総合研究大学院大学"}]}]}, "item_1_degree_name_6": {"attribute_name": "学位名", "attribute_value_mlt": [{"subitem_degreename": "博士（理学）"}]}, "item_1_description_1": {"attribute_name": "ID", "attribute_value_mlt": [{"subitem_description": "2003511", "subitem_description_type": "Other"}]}, "item_1_description_12": {"attribute_name": "要旨", "attribute_value_mlt": [{"subitem_description": "  The nucleocytoplasmic transport of macromolecules occurs through the nuclear pore complex (NPC).  Most transport events through the NPC are mediated by transport receptor molecules.  The localization of β-catenin to the nucleus is a crucial step in the transduction of the Wnt/Wingless signal.  Upon activation of the Wnt pathway, β-catenin accumulates in the nucleus.  Previous studies indicated that β-catenin could translocate on its own through the nuclear pores without the aid of small GTPase Ran, and shuttles between the cytoplasm and the nucleus.  The export of β-catenin also occurs in a Ran-independent manner.  Unlike receptor-mediated transport, β-catenin transport appears to use a rather unconventional mechanism, possibly through the direct interaction with NPC components.  In this study, I have analyzed the sequence requirement of β-catenin for its Ran-independent import and export in living mammalian cells and in the in vitro transport assay using semi-intact cells.  I confirmed that a β-catenin fragment containing both Armadillo repeats 10- 12 and the C-terminus possesses most strong nuclear import and export activity.  Further dissection of this fragment showed that C-terminus of β-catenin is most important for its import and export.  Moreover, I found that region required for β-catenin import and export overlaps, but they are not identical.  Competition studies using different transport receptors under different conditions indicated that interaction(s) required for β-catenin import and export differ at NPC.", "subitem_description_type": "Other"}]}, "item_1_description_18": {"attribute_name": "フォーマット", "attribute_value_mlt": [{"subitem_description": "application/pdf", "subitem_description_type": "Other"}]}, "item_1_description_7": {"attribute_name": "学位記番号", "attribute_value_mlt": [{"subitem_description": "総研大甲第723号", "subitem_description_type": "Other"}]}, "item_1_select_14": {"attribute_name": "所蔵", "attribute_value_mlt": [{"subitem_select_item": "有"}]}, "item_1_select_8": {"attribute_name": "研究科", "attribute_value_mlt": [{"subitem_select_item": "生命科学研究科"}]}, "item_1_select_9": {"attribute_name": "専攻", "attribute_value_mlt": [{"subitem_select_item": "18 遺伝学専攻"}]}, "item_1_text_10": {"attribute_name": "学位授与年度", "attribute_value_mlt": [{"subitem_text_value": "2003"}]}, "item_1_text_20": {"attribute_name": "業務メモ", "attribute_value_mlt": [{"subitem_text_value": "（2018年2月20日）本籍など個人情報の記載がある旧要旨・審査要旨を個人情報のない新しいものに差し替えた。承諾書等未確認。要確認該当項目修正のこと。"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "KOIKE, Makiko", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "10106", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-02-17"}], "displaytype": "simple", "download_preview_message": "", "file_order": 0, "filename": "甲723_要旨.pdf", "filesize": [{"value": "162.3 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 162300.0, "url": {"label": "要旨・審査要旨 / Abstract, Screening Result", "url": "https://ir.soken.ac.jp/record/989/files/甲723_要旨.pdf"}, "version_id": "e3f65cec-37b9-4523-8c54-32f2079e7472"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-02-17"}], "displaytype": "simple", "download_preview_message": "", "file_order": 1, "filename": "甲723_本文.pdf", "filesize": [{"value": "2.9 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 2900000.0, "url": {"label": "本文", "url": "https://ir.soken.ac.jp/record/989/files/甲723_本文.pdf"}, "version_id": "ed88166f-35fb-4c3d-a78e-25f87243687c"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "thesis", "resourceuri": "http://purl.org/coar/resource_type/c_46ec"}]}, "item_title": "Sequence determination of region required  for nuclear import and export of β-catenin: Implication for distinct molecular interactions involved in bi-directional nuclear pore passage.", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Sequence determination of region required  for nuclear import and export of β-catenin: Implication for distinct molecular interactions involved in bi-directional nuclear pore passage."}, {"subitem_title": "Sequence determination of region required for nuclear import and export of β-catenin: Implication for distinct molecular interactions involved in bi-directional nuclear pore passage.", "subitem_title_language": "en"}]}, "item_type_id": "1", "owner": "1", "path": ["20"], "permalink_uri": "https://ir.soken.ac.jp/records/989", "pubdate": {"attribute_name": "公開日", "attribute_value": "2010-02-22"}, "publish_date": "2010-02-22", "publish_status": "0", "recid": "989", "relation": {}, "relation_version_is_last": true, "title": ["Sequence determination of region required  for nuclear import and export of β-catenin: Implication for distinct molecular interactions involved in bi-directional nuclear pore passage."], "weko_shared_id": 1}